Purification and characterization of Streptomyces griseus trypsin overexpressed in Streptomyces lividans

Journal of Microbiology and Biotechnology

Volumn 8, Issue 4, 1998, Pages 333-340

  Koo, Bon Joon ^b   Bae, Kwang Hee ^c   Byun, Si Myong ^c   Hong, Soon Kwang ^a  

^a Myong Ji University   (South Korea)

^b TS Corporation   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Characterization; Purification; sprT; Streptomyces griseus trypsin (SGT); Streptomyces lividans

Indexed keywords

TRYPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; GENE OVEREXPRESSION; NONHUMAN; PROTEIN EXPRESSION; STREPTOMYCES GRISEUS; STREPTOMYCES LIVIDANS;

STREPTOMYCES GRISEUS; STREPTOMYCES LIVIDANS;

EID: 7344253535     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Amann, E., B. Ochs, and K. J. Abel. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene 69: 301-315.
2. Awad, W. M. Jr., K. Brew, J. T. Powell, D. J. Russin, J. F. Seber, S. Siegel, and K. D. Vosbeck. 1976. The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). XI. Evolutionary Implications, pp. 77-91.
3. Bender, E., R. Vogel, K. P. Koller, and J. Engels. 1990. Synthesis and secretion of hirudin by Streptomyces lividans. Appl. Microbial. Biotechnol. 34: 203-207.
4. Bibb, M. J., P. R. Findlay, and M. W. Johnson. 1984. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences. Gene 30: 157-166.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
6. Chandrasekaran, S. and S. C. Dhar. 1987. Multiple proteases from Streptomyces moderatus. I. Isolation and purification of five extracellular proteases. Arch. Biochem. Biophys. 257: 395-401.
7. Daza, A., J. A. Gil, T. Vigal, and J. F. Martin. 1990. Cloning and characterization of a gene of Streptomyces griseus that increases production of extracellular enzymes in several species of Streptomyces. Mol. Gen. Genet. 222: 384-392.
8. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580.
9. Henderson, G., P. Krygsman, C. J. Liu, C. C. Davey, and L. T. Malek. 1987. Characterization and structure of genes for proteases A and B from Streptomyces griseus. J. Bacteriol. 169: 3778-3784.
10. Hong, Y. S., C. K. Hwang, D. Y. Hwang, Y. H. Kim, S. J. kim, and J. J. Lee. 1993. Cloning and sequencing of a gene cluster for the resistance to doxorubicin from Streptomyces peucetius subsp. caesius ATCC 27952. J. Microbiol. Biotechnol. 2: 153-160.
11. Hopwood, D. A., M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, and J. M. Ward. 1985. Genetic Manipulation of Streptomyces: A Laboratory Manual, The John Innes Foundation, Norwich, England.
12. Kim, I. S. and K. J. Lee. 1996. Trypsin-like protease of Streptomyces exfoliatus SMF13, a potential agent in mycelial differentiation. Microbiology 142: 1797-1806.
13. Kim, J. C., S. H. Cha, S. T. Jeong, S. K. Oh, and S. M. Byun. 1991. Molecular cloning and nucleotide sequence of Streptomyces griseus trypsin gene. Biochem. Biophys. Res. Commun. 181: 707-713.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
15. Lichenstein, H., M. E. Brawner, L. M. Miles, C. A. Meyers, P. R. Young, P. L. Simon, and T. Eckhardt. 1988. Secretion of interleukin-1 beta and Escherichia coli galactokinase by Streptomyces lividans. J. Bacteriol. 170: 3924-3929.
16. Makoff, A. J., M. D. Oxer, M. A. Romanos, N. F. Fairweather, and S. Ballantine. 1989. Expression of tetanus toxin fragment C in E. coli: High level expression by removing rare codons. Nucleic Acid. Res. 17: 10191-10202.
17. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A.
18. Moncheva, P. A., S. T. Danova, S. K. Antonova, and I. V. Ivanova. 1997. Physiological role of extracellular proteases and calcium ions in the processes of differentiation and antibiotic production by Streptomyces albogriseolus 444. Antibiot. Khimioter. 42: 14-19.
19. Narahashi, Y., K. Shibuya, and M. Yanagita. 1968. Studies on proteolytic enzymes (pronase) of Streptomyces griseus K-1. II. Separation of exo- and endopeptidases of pronase. J. Biochem. (Tokyo) 64: 427-437.
20. Olafson, R. W., L. Jurasek, M. R. Carpenter, and L. B. Smillie. 1975. Amino acid sequence of Streptomyces griseus trypsin. Cyanogen bromide fragments and complete sequence. Biochemistry 14: 1168- 1177.
21. Read, R. J. and M. N. James. 1988. Refined crystal structure of Streptomyces griseus trypsin at 1.7 Å resolution. J. Mol. Biol. 200: 523-551.
22. Robinson, M., E. Lewis, and E. Napier. 1981. Occurrence of reiterated DNA sequences in strains of Streptomyces produced by an interspecific protoplast fusion. Mol. Gen. Genet. 182: 336-340.
23. Tomich, P. K. 1988. Streptomyces cloning: Useful recombinant DNA systems and a summation of cloned genes. Antimicrob. Agents Chemother. 32: 1465-1471.
24. Trop, M. and Y. Birk. 1970. The specificity of proteinases from Streptomyces griseus (pronase). J. Biochem. 116: 19-25.
25. Trop, M. and Y. Birk. 1968. The trypsin-like enzyme from Streptomyces griseus (pronase). J. Biochem. 109: 475-476.
26. Tsuyuki, H., K. Kajiwara, A. Fujita, T. Kumazaki, and S. Ishii. 1991. Purification and characterization of Streptomyces griseus metalloendopeptidases I and II. J. Biochem. (Tokyo) 110: 339-344.
27. Vara, J., M. Lewandowska-Skarbek, Y. G. Wang, S. Donadio, and C. R. Hutchinson. 1989. Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J. Bacteriol. 171: 5872-5881.
28. Vieira, J. and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268.
29. Vosbeck, K. D., B. D. Greenberg, M. S. Ochoa, P. L. Whitney, and W. M. Jr. Awad. 1978. Proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). Effect of pH, metal ions, and amino acids on aminopeptidase activity. J. Biol. Chem. 253: 257-260.
30. Wandersman, C. 1989. Secretion, processing, and activation of bacterial extracellular proteases. Mol. Microbiol. 3: 1825-1831.
31. Yoshida, N., S. Tsuruyama, K. Nagata, K. Hirayama, K. Noda, and S. Makisumi. 1988. Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase). J. Biochem. (Tokyo) 104: 451-456.