Medium osmolarity and pericellular matrix development improves chondrocyte survival when photoencapsulated in poly(ethylene glycol) hydrogels at low densities

Tissue Engineering - Part A

Volumn 15, Issue 10, 2009, Pages 3037-3048

  Villanueva, Idalis ^a   Bishop, Nikki L ^a   Bryant, Stephanie J ^a  

^a UCB 450   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; BODY FLUIDS; CELLS; ETHYLENE; ETHYLENE GLYCOL; FREE RADICALS; HYDROGELS; PHYSIOLOGY; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES;

BOVINE CHONDROCYTES; CELL DENSITY; CELL NUMBER; CELL SOURCES; CELL SURVIVAL; CHONDROCYTES; ENZYMATIC DIGESTIONS; FREE-RADICAL DAMAGE; LOW DENSITY; MICROENVIRONMENTS; OSMOLARITY; PERICELLULAR MATRIX; PHOSPHATE-BUFFERED SALINES; POLY(ETHYLENE GLYCOL) HYDROGEL;

CYTOLOGY;

BOVINAE;

MACROGOL DERIVATIVE;

ANIMAL; APOPTOSIS; ARTICLE; CARTILAGE CELL; CATTLE; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; EXTRACELLULAR MATRIX; HYDROGEL; METABOLISM; METHODOLOGY; OSMOLARITY; PHYSIOLOGY; TISSUE ENGINEERING;

ANIMALS; APOPTOSIS; CATTLE; CELL SURVIVAL; CELLS, CULTURED; CHONDROCYTES; EXTRACELLULAR MATRIX; HYDROGELS; OSMOLAR CONCENTRATION; POLYETHYLENE GLYCOLS; TISSUE ENGINEERING;

EID: 73349141193     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0001     Document Type: Article

References (45)

1. Mikos, A.G., Herring, S.W., Ochareon, P., Elisseeff, J., Lu, H.H., Kandel, R., Schoen, F.J., Toner, M., Mooney, D., Atala, A., van Dyke, M.E., Kaplan, D., and Vunjak-Novakovic, G. Engineering complex tissues. Tissue Eng 12, 3307, 2006.
2. Nicodemus, G.D., and Bryant, SJ. Cell encapsulation in biodegradable hydrogels for tissue engineering applications. Tissue Eng 14, 149, 2008.
3. Ifcovits, J.L., and Burdick, J.A. Review: photopolymerizable and degradable biomaterials for tissue engineering applica-tions. Tissue Eng 13, 2369, 2007.
4. Burdick, J.A., and Anseth, K.S. Photoencapsulation of oste-oblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. Biomaterials 23, 4315, 2002.
5. Sawhney, A.S., Pathak, C.P., and Hubbell, J.A. Modification of islet of Langerhans surfaces with immunoprotective poly (ethylene glycol) coatings via interfacial photopolymerization. Biotechnol Bioeng 44, 383,1994.
6. Elisseeff, J., McIntosh, W., Anseth, K., Riley, S., Ragan, P., and Langer, R. Photoencapsulation of chondrocytes in poly(ethylene oxide)-based semi-interpenetrating networks. J Biomed Mater Res 51, 164, 2000.
7. Bryant, S.J., and Anseth, K.S. The effects of scaffold thickness on tissue engineered cartilage in photocrosslinked poly(eth-ylene oxide) hydrogels. Biomaterials 22, 619, 2001.
8. Nuttelman, C.R., Tripodi, M.C., and Anseth, K.S. In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels. J Biomed Mater Res A 68A, 773, 2004.
9. Goodner, M.D., Lee, H.R., and Bowman, C.N. Method for determining the kinetic parameters in diffusion-controlled free-radical homopolymerizations. Ind Eng Chem Res 36, 1247, 1997.
10. Dröge, W. Free radicals in the physiological control of cell function. Physiol Rev 82, 47, 2002.
11. Halliwell, B., and Chirico, S. Lipid peroxidation: its mecha-nism, measurement and significance. Am J Clin Nutr 57, 715S, 1993.
12. Terakado, M., Yamazaki, M., Tsujimoto, Y., Kawashima, T., Nagashima, K., Ogawa, J., Fujita, Y., Sugiya, H., Sakai, T., and Furuyama, S. Lipid peroxidation as a possible cause of benzoyl peroxide toxicity in rabbit dental pulp\a micro-somal lipid peroxidation in vitro. J Dent Res 63, 901, 1984.
13. Hanks, C.T., Strawn, S.E., Wataha, J.C., and Craig, R.G. Cytotoxic effects of resin components on cultured mamma-lian fibroblasts. J Dent Res 70, 1450, 1991.
14. Atsumi, T., Murata, J., Kamiyanagi, I., Fujisawa, S., and Ueha, T. Cytotoxicity of photosensitizers camphorquinone and 9-fluorenone with visible light irradiation on a human submandibular-duct cell line in vitro. Arch Oral Biol 43, 73, 1998.
15. Bryant, S.J., Nuttelman, C.R., and Anseth, K.S. Cyto-compatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro. J Biomater Sci Polymer Ed 11, 439, 2000.
16. Fedorovich, N.E., Oudshoorn, M.H., van Geemen, D., Hennink, W.E., Alblas, J., and Dhert, W.J. The effect of photopolymerization on stem cells embedded in hydrogels. Biomaterials 30, 344, 2009.
17. Mayne, R., Vail, M.S., Mayne, P.M., and Miller, E.J. Changes in type of collagen synthesized as clones of chick chon-drocytes grow and eventually lose division capacity. Proc Natl Acad Sci USA 73, 1674, 1976.
18. Aulthouse, A.L., Beck, M., Griffey, E., Sanford, J., Arden, K., Machado, M.A., and Horton, W.A. Expression of the human chondrocyte phenotype in vitro. In Vitro Cell Dev Biol 25, 659, 1989.
19. de Haart, M., Marijnissen, W.J., van Osch, G.J., and Verhaar, J.A. Optimisation of chondrocyte expansion in culture. Effect of TGFß2, bFGF and L-ascorbic acid on bovine articular chondrocytes. Acta Orthop Scand 70, 55, 1999.
20. Bryant, S.J., and Anseth, K.S. Controlling the spatial distri-bution of ECM components in degradable PEG hydrogels for tissue engineering cartilage. J Biomed Mater Res 64, 70, 2003.
21. Nicodemus, G.D., Villanueva, I., and Bryant, S.J. Mechan-ical stimulation of TMJ condylar chondrocytes encapsu-lated in PEG hydrogels. J Biomed Mater Res A 83A, 323, 2007.
22. Villanueva, I., Hauschulz, D.S., Mejic, D., and Bryant, S.J. Static and dynamic compressive strains influence nitric ox-ide production and chondrocyte bioactivity when encapsulated in PEG hydrogels of different crosslinking densities. Osteoarthritis Cartilage 16, 909, 2008.
23. Temenoff, J.S., Park, H., Jabbari, E., Sheffield, T.L., LeBaron, R.G., Ambrose, C.G., and Mikos, A.G. In vitro osteogenic differentiation of marrow stromal cells encapsulated in biodegradable hydrogels. J Biomed Mater Res A 70A, 235, 2004.
24. Suggs, L.J., and Mikos, A.G. Development of poly(propylene fumarate-co-ethylene glycol) as an injectable carrier for en-dothelial cells. Cell Transplant 8, 345, 1999.
25. Knight, M.M., Lee, D.A., and Bader, D.L. Distribution of chondrocyte deformation in compressed agarose gel using confocal microscopy. J Cell Eng 1, 97, 1996.
26. Lee, D.A., and Bader, D.L. The development and char-acterisation of an in vitro system to study strain induced cell deformation in isolated chondrocytes. In Vitro Cell Dev Biol 31, 828, 1995.
27. Knight, M.M., Ghori, S.A., Lee, D.A., and Bader, D.L. Mea-surement of the deformation of isolated chondrocytes in agarose subjected to cyclic compression. Med Eng Phys 20, 684, 1998.
28. Urban, J.P.G., Hall, A.C., and Gehl, K.A. Regulation of ma-trix synthesis rates by the ionic and osmotic environment of articular chondrocytes. J Cell Physiol 154, 262, 1993.
29. Machlin, L.J., and Bendich, A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J 1, 441, 1987.
30. Xiao, H.L., Cai, G.P., and Liu, M.Y. Hydroxyl radical in-duced structural changes of collagen. Spectrosc Int J21,91, 2007.
31. McCabe, R.D., Bakarich, M.A., Srivastava, K., and Young, D.B. Potassium inhibits free radical formation. Hypertension 24, 77, 1994.
32. Burton, G.W., and Ingold, K.V. Beta carotene: an unusual type of lipid antioxidant. Science 224, 569, 1984.
33. Lin-Gibson, S., Bencherif, S., Cooper, J.A., Wetzel, S.J., An-tonucci, J.M., Vogel, B.M., Horkay, F., and Washburn, N.R. Synthesis and characterization of PEG dimethacrylates and their hydrogels. Biomacromolecules 5, 1280, 2004.
34. Lee, D.A., and Bader, D.L. Compressive strains at physio-logical frequencies influence the metabolism of chondrocytes seeded in agarose. J Orthop Res 15, 181, 1997.
35. Farndale, R.W., Buttle, D.J., and Barrett, A.J. Improved quantitation and discrimination of sulfated glycosamino-glycans by use of dimethylmethylene blue. Biochim Biophys Acta 883, 173, 1986.
36. Kim, Y.J., Sah, R.L.Y., Doong, J.Y.H., and Grodzinsky, A.J. Fluorometric assay of DNA in cartilage explants using Hoechst-33258. Anal Biochem 174, 168, 1988.
37. Bryant, S.J., and Anseth, K.S. Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res 59, 63, 2001.
38. Lovell, L.G., Newman, S.M., and Bowman, C.N. The effects of light intensity, temperature, and comonomer composition on the polymerization behavior of dimethacrylate dental resins. J Dent Res 78, 1469, 1999.
39. Bryant, S.J., and Anseth, K.S. Hydrogel properties influence ECM production by chondrocyte photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res 59, 63, 2002.
40. Quick, D.J., and Anseth, K.S. DNA delivery from photo-crosslinked PEG hydrogels: encapsulation efficiency, release profiles, and DNA quality. J Control Release 96, 341, 2004.
41. Odland, L., Wallin, S., and Walum, E. Lipid peroxidation and activities of aminotransferase and glutamine synthetase in hepatoma and glioma cells grown in bovine colostrum-supplemented medium. In Vitro 22, 259, 1986.
42. Okada, Y. Ion channels and transporters involved in cell volume regulation and sensor mechanisms. Cell Biochem Biophys 41, 233, 2004.
43. Guilak, F., Alexopoulos, L.G., Upton, M.L., Youn, I., Choi, J.B., Cao, L., Setton, L.A., and Haider, M.A. The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage. Ann NY Acad Sci 1068, 498, 2006.
44. Hing, W.A., Sherwin, A.F., Ross, J.M., and Poole, C.A. The influence of the pericellular microenvironment on the chondrocyte response to osmotic challenge. Osteoarthritis Cartilage 10, 297, 2002.
45. Negoro, K., Kobayashi, S., Takeno, K., Uchida, K., and Baba, H. Effect of osmolarity on glycosaminoglycan production and cell metabolism of articular chondrocyte under three-dimensional culture system. Clin Exp Rheumatol 26, 534, 2008