Metabolic profiling of maize mutants deficient for two glutamine synthetase isoenzymes using1H-NMR-based metabolomics

Phytochemical Analysis

Volumn 21, Issue 1, 2010, Pages 102-109

  Broyart, Caroline ^a,b   Fontaine, Jean Xavier ^a   Molinié, Roland ^a   Cailleu, Dominique ^c   Tercé Laforgue, Thérèse ^b   Dubois, Frédéric ^a   Hirel, Bertrand ^b   Mesnard, François ^a  

^a UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^b CEMAGREF   (France)

^c UFR des Sciences Bâtiment Serres Transfert Rue Dallery   (France)

Author keywords

Glutamine synthetase; Metabolomics; Mutants; Nuclear magnetic resonance spectroscopy; Zea mays

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; GENE EXPRESSION; GRAIN (AGRICULTURAL PRODUCT); METABOLISM; NITROGEN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

CYTOSOLIC; DOUBLE MUTANTS; GENES ENCODING; GLUTAMINE SYNTHETASE; MAIZE MUTANTS; METABOLIC PROFILES; METABOLIC PROFILING; METABOLOMICS; MUTANT; ZEA MAYS;

METABOLITES;

ALANINE; ASPARAGINE; GLUTAMATE AMMONIA LIGASE; GLUTAMATE AMMONIA LIGASE 1; GLUTAMATE AMMONIA LIGASE 2; GLUTAMATE AMMONIA LIGASE 3; GLUTAMATE AMMONIA LIGASE 4; ISOENZYME; LIGNIN; NITROGEN; PHENYLALANINE; PHOSPHATIDYLCHOLINE; THREONINE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE MUTATION; GRAIN YIELD; MAIZE; METABOLOMICS; NONHUMAN; PHYTOCHEMISTRY; PLANT LEAF; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE; SEED KERNEL; SHOOT;

ZEA MAYS;

EID: 73349125774     PISSN: 09580344     EISSN: 10991565     Source Type: Journal    
DOI: 10.1002/pca.1177     Document Type: Article

References (45)

1. Andrews M, Lea PJ, Raven JA, Lindsey K. 2004. Can genetic manipulation of plant nitrogen assimilation enzymes result in increased crop yield and greater N-use efficiency? An assessment. Ann Appl Biol 145: 25-40.
2. Bernard SM, Habash DZ. 2009. The importance of cytosolic glutamine synthetase in nitrogen assimilation and recycling. New Phytol 182: 608-620.
3. Bernard SM, Møller ALB, Dionisio G, Kichey T, Jahn TP, Dubois F, Baudo M, Lopes MS, Tercé-Laforgue T, Foyer CH, Parry MAJ, Forde BG, Araus JL, Hirel B, Schjoerring JK, Habash DZ. 2008. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.). Plant Mol Biol 67: 89-105.
4. Brugière N, Dubois F, Limami AM, Lelandais M, Roux Y, Sangwan RS, Hirel B. 1999. Glutamine synthetase in the phloem plays a major role in controlling proline production. Plant Cell 11: 1995-2012.
5. Brugière N, Dubois F, Masclaux C, Sangwan RS, Hirel B. 2000. Immunolocalization of glutamine synthetase in senescing tobacco (Nicotiana tabacum L.) leaves suggests that ammonia assimilation is progressively shifted to the mesophyll cytosol. Planta 211: 519-527.
6. Cañas RA, Quilleré I, Christ A, Hirel B. 2009. Nitrogen metabolism in the developing ear of maize (Zea mays L.): analysis of two lines contrasting in their mode of nitrogen management. New Phytol 184: 340-352.
7. Cánovas FM, Avila C, Cantón FR, Cañas RA, de la Torre F. 2007. Ammonium assimilation and amino acid metabolism in conifers. J Exp Bot 58: 2307-2318.
8. Carvalho H, Sunkel C, Salema R, Cullimore JV. 1997. Heteromeric assembly of the cytosolic glutamine synthetase polypeptides of Medicago truncatula: complementation of a glnA Escherichia coli mutant with a plant domain-swapped enzyme. Plant Mol Biol 35: 623-632.
9. Carvalho HG, Lopes-Cardoso IA, Lima LM, Melo PM, Cullimore JV. 2003. Nodule-specific modulation of glutamine synthetase in transgenic Medicago truncatula leads to inverse alterations in asparagine synthetase expression. Plant Physiol 133: 243-252.
10. Castro C, Motto M, Rossi V, Manetti C. 2008. Variation of metabolic profiles in developing maize kernels up- and down-regulated for the hda101 gene. J Exp Bot 59: 3913-3924.
11. Cren M, Hirel B. 1999. Glutamine synthetase in higher plants regulation of gene and protein expression from the organ to the cell. Plant Cell Physiol 40: 1187-1193.
12. Christensen LE, Below FE, Hageman RH. 1981. The effects of ear removal on senescence and metabolism of maize 1. Plant Physiol 68: 1180-1185.
13. Dubois F, Brugière N, Sangwan RS, Hirel B. 1996. Localization of tobacco cytosolic glutamine synthetase enzymes and the corresponding transcripts shows organ- and cell-specific patterns of protein synthesis and gene expression. Plant Mol Biol 31: 803-817.
14. Eriksson L, Johansson E, Kettaneh-Wold N, Wold S. 2001. Multi- and Megavariate Data Analysis: Principles and Applications. Umetrics: Umeå.
15. Forde BG, Lea PJ. 2007. Glutamate in plants: metabolism, regulation, and signalling. J Exp Bot 58: 2339-2358.
16. Gallais A, Hirel B. 2004. An approach to the genetics of nitrogen use efficiency in maize. J Exp Bot 55: 295-306.
17. Habash DZ, Bernard S, Schondelmaier J, Weyen J, Quarrie SA. 2007. The genetics of nitrogen use in hexaploid wheat: N utilisation, development and yield. Theor Appl Genet 114: 403-419.
18. Harrison J, Pou de Crescenzo MA, Sené O, Hirel B. 2003. Does lowering glutamine synthetase activity in nodules modify nitrogen metabolism and growth of Lotus japonicus? Plant Physiol 133: 253-262.
19. Hirel B, Lea P. 2001. Ammonium assimilation. In Plant Nitrogen, Lea PJ, Morot-Gaudry JF (eds). Springer: Berlin; 79-99.
20. Hirel B, Martin A, Tercé-Laforgue T, Gonzalez-Moro MB, Estavillo JM. 2005. Physiology of maize I: A comprehensive and integrated view of nitrogen metabolism in a C4 plant. Physiol Plant 124: 167-177.
21. Hirel B, Le Gouis J, Ney B, Gallais A. 2007. The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches. J Exp Bot 58: 2369-2387.
22. Hörtensteiner S, Feller U. 2002. Nitrogen metabolism and remobilization during senescence. J Exp Bot 53: 927-937.
23. Ireland RJ, Lea PJ. 1999. The enzymes of glutamine, glutamate, asparagine and aspartate metabolism. In Plant Amino Acids: Biochemistry and Biotechnology, Singh BK (ed.). Marcel Dekker: New York; 49-110.
24. Ishiyama K, Inoue E, Watanabe-Takahashi A, Obara M, Yamaya T, Takahashi H. 2004. Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis. J Biol Chem 279: 16598-16605.
25. Kichey T, Le Gouis J, Sangwan B, Hirel B, Dubois F. 2005. Changes in the cellular and subcellular localization of glutamine synthetase and glutamate dehydrogenase during flag leaf senescence in wheat (Triticum aestivum L.). Plant Cell Physiol 46: 964-974.
26. Labboun S, Tercé-Laforgue T, Roscher A, Bedu M, Restivo FM, Velanis CN, Skopelitis DS, Moshou PN, Roubelakis KA, Suzuki A, Hirel B. 2009. Resolving the role of plant glutamate dehydrogenase: I. In vivo real time nuclear magnetic resonance spectroscopy experiments. Plant Cell Physiol 50: 1761-1773.
27. Lea P, Sodek L, Parry M, Shewry P, Halford N. 2007. Asparagine in plants. Ann Appl Biol 150: 1-26.
28. Leiss KA, Maltese F, Choi YH, Verpoorte R, Klinkhamer PGL. 2009. Identification of chlorogenic Acid as a resistance factor for thrips in chrysanthemum. Plant Physiol 150: 1567-1575.
29. Li MG, Villemur R, Hussey PJ, Silflow CD, Gantt JS, Snustad DP. 1993. Differential expression of six glutamine synthetase genes in Zea mays. Plant Mol Biol 23: 401-407.
30. Limami AM, Rouillon C, Glevarec G, Gallais A, Hirel B. 2002. Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase. Plant Physiol 130: 1860-1870.
31. Manetti C, Bianchetti C, Casciani L, Castro C, Cocco MED, Miccheli A, Motto M, Conti F. 2006. A metabonomic study of transgenic maize (Zea mays) seeds revealed variations in osmolytes and branched amino acids. J Exp Bot 57: 2613-2625.
32. Martin A, Belastegui-Macadam X, Quilleré I, Floriot M, Valadier MH, Pommel B, Andrieu B, Donnison I, Hirel B. 2005. Nitrogen management and senescence in two maize hybrids differing in the persistence of leaf greenness. Agronomic, physiological and molecular aspects. New Phytol. 167: 483-492.
33. Martin A, Lee J, Kichey T, Gerentes D, Zivy M, Tatout C, Dubois F, Balliau T, Valot B, Davanture M, Tercé-Laforgue T, Quilleré I, Coque M, Gallais A, Gonzalez-Moro MB, Bethencourt L, Habash DZ, Lea PJ, Charcosset A, Perez P, Murigneux A, Sakakibara H, Edwards KJ, Hirel B. 2006. Two cytosolic glutamine synthetase isoforms of maize are specifically involved in the control of grain production. Plant Cell 18: 3252-3274.
34. Masclaux C, Quilleré I, Gallais A, Hirel B. 2001. The challenge of remobilization in plant nitrogen economy. A survey of physio-agronomic and molecular approaches. Ann Appl Biol 138: 69-81.
35. Miflin BJ, Habash DZ. 2002. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J Exp Bot 53: 979-987.
36. Piccioni F, Capitani D, Zolla L, Mannina L. 2009. NMR metabolic profiling of transgenic maize with the Cry1A(b) gene. J Agric Food Chem 57: 6041-6049.
37. Reeves RG.1950. Morphology of the ear and tassel of maize. Am J Bot 37: 697-704.
38. Sakakibara H, Kawabata S, Hase T, Sugiyama T. 1992. Differential effects of nitrate and light on the expression of glutamine synthetases and ferredoxin-dependent glutamate synthase in maize. Plant Cell Physiol 33: 1193-1198.
39. Sakurai N, Hayakawa T, Nakamura T, Yamaya T. 1996. Changes in the cellular localization of cytosolic glutamine synthetase protein in vascular bundles of rice leaves at various stages of development. Planta 200: 306-311.
40. Tabuchi M, Sugiyama K, Ishiyama K, Inoue E, Sato T, Takahashi H, Yamaya T. 2005. Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1. Plant J 42: 641-651.
41. Teixeira J, Pereira S, Cánovas F, Salema R. 2005. Glutamine synthetase of potato (Solanum tuberosum L. cv. Desiree) plants: cell- and organ-specific expression and differential developmental regulation reveal specific roles in nitrogen assimilation and mobilization. J Exp Bot 56: 663-671.
42. Tobin AK, Yamaya T. 2001. Cellular compartmentation of ammonium assimilation in rice and barley. J Exp Bot 52: 591-604.
43. Verpoorte R, Choi YH, Kim HK. 2007. NMR-based metabolomics at work in phytochemistry. Phytochem Rev 6: 3-14.
44. Whetten R, SederoffR. 1995. Lignin biosynthesis. Plant Cell 7: 1001-1013.
45. Wong HK, Chan HK, Coruzzi GM, Lam HM. 2004. Correlation of ASN2 gene expression with ammonium metabolism in Arabidopsis. Plant Physiol 134: 332-338.