Low vacuum scanning electron microscopy for paraffin sections utilizing the differential stainability of cells and tissues with platinum blue

Archives of Histology and Cytology

Volumn 72, Issue 2, 2009, Pages 101-106

  Inaga, Sumire ^a   Hirashima, Sayuri ^b   Tanaka, Keiichi ^c   Katsumoto, Tetsuo ^a   Kameie, Toshio ^a   Nakane, Hironobu ^a   Naguro, Tomonori ^a  

^a TOTTORI UNIVERSITY   (Japan)

^b HITACHI HIGH TECHNOLOGIES CORPORATION   (Japan)

^c Tanaka SEM Institute   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; COLLAGEN; COLORING AGENTS; ELASTIC TISSUE; ENDOTHELIUM; HYDROGEN-ION CONCENTRATION; MALE; MAST CELLS; MICROSCOPY, ELECTRON, SCANNING; ORGANOPLATINUM COMPOUNDS; PARAFFIN EMBEDDING; RATS; STAINING AND LABELING; THYMIDINE; VACUUM;

EID: 73349109520     PISSN: 09149465     EISSN: 13491717     Source Type: Journal    
DOI: 10.1679/aohc.72.101     Document Type: Article

References (8)

1. Inaga S, Tanaka K, Iino A: Three-dimensional helical coiling structures and band patterns of hydrous metaphase chromosomes observed by low vacuum scanning electron microscopy. Arch Histol Cytol 65: 415-423 (2002).
2. Inaga S, Katsumoto T, Tanaka K, Kameie T, Nakane H, Naguro T: Platinum blue as an alternative to uranyl acetate for staining of transmission electron microscopy. Arch Histol Cytol 70: 43-49 (2007a).
3. Inaga S, Katsumoto T, Tanaka K, Kameie T, Nakane H, Naguro T: Applications of platinum blue as an electron stain for transmission electron microscopy. Acta Anat Nippon 82 (Supple): 149 (2007b) (in Japanese).
4. Inaga S, Hirashima S, Takei S, Katsumoto T, Kameie T, Nakane H, Naguro T, Tanaka K: Characteristic of stainability and staining conditions of platinum blue as an electron stain for electron microscopy. Acta Anat Nippon 83 (Supple): 162 (2008) (in Japanese).
5. Kato S, Gotoh M: Application of backscattered electron imaging to enzyme-histochemistry of lymphatic capillaries. J Electron Microsc 39: 186-190 (1990).
6. Tanaka K, Inagaki K: Enhancement of the BSE signal from hydrous SEM samples by use of a platinum blue. J Electron Microsc 42: 255 (1993).
7. Tanaka K, Inaga S, Iino A: New techniques for observing hydrous biological materials by low vacuum scanning electron microscopy and their applications. In: Recent advances in microscopy of cells, tissue and organs (Motta P, ed), Antonio Delfiro Editore, Rome, 1997 (p. 31-35).
8. Tanaka K, Inaga S, Iino A, Ushiki T, Saito S: Fine structure of hydrous chromosomes observed by low vacuum scanning electron microscope. Arch Histol Cytol 61: 337-342 (1998).