High-resolution melting analysis (HRMA): A highly sensitive inexpensive genotyping alternative for population studies

Molecular Ecology Resources

Volumn 10, Issue 1, 2010, Pages 193-196

  Smith, B L ^a   Lu, C P ^a   Alvarado Bremer, J R ^a,b  

^a TEXAS A AND M UNIVERSITY   (United States)

^b TEXAS A AND M UNIVERSITY AT GALVESTON   (United States)

Author keywords

Genotyping; High resolution melting analysis; Nuclear DNA; Population studies; SNP; Swordfish

Indexed keywords

XIPHIAS GLADIUS;

EID: 73349109122     PISSN: 1755098X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1755-0998.2009.02726.x     Document Type: Article

References (16)

1. Alvarado Bremer JR, Mejuto J, Gómez-Márquez J et al. (2005) Hierarchical analyses of genetic variation of samples from breeding grounds and feeding grounds confirm genetic partitioning northwest Atlantic and South Atlantic population of swordfish (Xiphias gladius L.). Journal of Experimental Marine Biology and Ecology, 327, 167 182.
2. Alvarado Bremer JR, Hinton MG, Grieg TW (2006) Evidence of spatial genetic heterogeneity in Pacific swordfish (Xiphias gladius) revealed by the analysis of ldh-A sequences. Bulletin of Marine Science, 79, 493 503.
3. Atarhouch T, Rami M, Cattaneo-Berrebi G et al. (2003) New primers for EPIC amplification of intron sequences for fish and other vertebrate population genetic studies. Bio Techniques, 35, 676 682.
4. Chow S (1998) Universal PCR primer for calmodulin gene intron in fish. Fisheries Science, 64, 999 1000.
5. Graham R, Liew M, Meadows C, Lyon E, Wittwer CT (2005) Distinguishing different DNA heterozygotes by high-resolution melting. Clinical Chemistry, 51, 1295 1298.
6. Grieg TW 2000) Partitioning genetic variation in swordfish (Xiphias gladius L.); analysis of sample variance and population structure. Doctoral Dissertation. University of South Carolina, Columbia.
7. Gundry CN, Dobrowolski SF, Martin YR et al. (2008) Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons. Nucleic Acids Research, 36, 3401 3408.
8. Herrmann MG, Durtschi JD, Wittwer CT, Voelkerding KV (2007) Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping. Clinical Chemistry, 53, 1544 1548.
9. Kwok PY (2003) Single Nucleotide Polymorphisms - Methods and Protocols. Humana Press, Totowa, NJ.
10. Lee C, Kim J, Bae S et al. (2008) Identification of mycobacteria using high resolution amplicon DNA melting analysis. Clinical Chemistry, 54, A160 A160.
11. Liew M, Seipp M, Durtschi J et al. (2007) Closed-tube SNP genotyping without labeled probes a comparison between unlabeled probe and amplicon melting. American Journal of Clinical Pathology, 127, 341 348.
12. Palais RA, Liew MA, Wittwer CT (2005) Quantitative heteroduplex anaylsis for single nucleotide polymorphism genotyping. Analytical Biochemistry, 346, 167 175.
13. Poulson MD, Wittwer CT (2007) Closed-tube genotyping of apolipoprotein E by isolated-probe PCR with multiple unlabeled probes and high-resolution DNA melting analysis. Biotechniques, 43, 87 91.
14. Reed GH, Kent JO, Wittwer CT (2007) High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics, 8, 597 608.
15. Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M (2007) Evolution of ZZ/ZW and XX/XY sex-determination systems in the closely related medaka species, Oryzias hubbsi and O. dancena. Chromosoma, 116, 463 470.
16. Vandersteen JG, Bayrak-Toydemir P, Palais RA, Wittwer CT (2007) Identifying common genetic variants by high-resolution melting. Clinical Chemistry, 53, 1191 1198.