Preparation of extracellular domain 3 of human VEGF receptor-2 and the monitoring of its real-time binding to VEGF by biosensors

Biotechnology Progress

Volumn 25, Issue 6, 2009, Pages 1703-1708

  Zhang, Juan ^a,b   Li, Haixin ^a   Chen, Wei ^a   Cao, Peng ^c   Wang, Min ^a  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

^b SOUTHEAST UNIVERSITY   (China)

^c Jiangsu Province Institute of Traditional Chinese Medicine   (China)

Author keywords

Angiogenesis; Biosensor; KDR; VEGF

Indexed keywords

ANGIOGENESIS; ASSOCIATION RATE CONSTANTS; BIOLOGICAL ACTIVITIES; CATION EXCHANGE CHROMATOGRAPHY; CLINICAL APPLICATION; DISSOCIATION CONSTANT; DISSOCIATION RATES; EXPERIMENTAL CONDITIONS; EXTRACELLULAR DOMAINS; HIGH YIELD; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; KINETIC STUDY; LABEL-FREE BIOSENSOR; OPTICAL WAVEGUIDE LIGHTMODE SPECTROSCOPY; TUMOR ANGIOGENESIS; TUMOR GROWTH; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR; VEGFR-2;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; BLOOD VESSELS; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; CYTOLOGY; DISSOCIATION; ENDOTHELIAL CELLS; ESCHERICHIA COLI; METAL REFINING; RATE CONSTANTS; SELF ASSEMBLY; TUMORS;

BIOSENSORS;

PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; VASCULOTROPIN A; VASCULOTROPIN RECEPTOR 2;

ARTICLE; CELL CULTURE; CELL PROLIFERATION; ESCHERICHIA COLI; GENETIC PROCEDURES; GENETICS; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; SPECTROSCOPY; WESTERN BLOTTING;

BIOSENSING TECHNIQUES; BLOTTING, WESTERN; CELL PROLIFERATION; CELLS, CULTURED; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; KINETICS; PEPTIDE FRAGMENTS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2;

ESCHERICHIA COLI;

EID: 73249131824     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.252     Document Type: Article

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