P2X7 receptor activates multiple selective dye-permeation pathways in RAW 264.7 and human embryonic kidney 293 cells

Molecular Pharmacology

Volumn 76, Issue 6, 2009, Pages 1323-1332

  Cankurtaran Sayar, Serife ^a   Sayar, Kemal ^a   Ugur, Mehmet ^a  

^a ANKARA UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCEIN; CALCIUM ION; ETHIDIUM; FLUORESCENT DYE; LUCIFER YELLOW; PURINERGIC P2X7 RECEPTOR;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CELL MEMBRANE PERMEABILITY; CELL STRAIN HEK293; CONTROLLED STUDY; EXTRACELLULAR CALCIUM; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ADENOSINE TRIPHOSPHATE; ANIMALS; BENZOXAZOLES; CALCIUM; CELL LINE; CELL MEMBRANE PERMEABILITY; FLUORESCENT DYES; HUMANS; ISOQUINOLINES; KIDNEY; MACROPHAGES; QUINOLINIUM COMPOUNDS; RATS; RECEPTORS, PURINERGIC P2; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION;

EID: 73149109743     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.109.059923     Document Type: Article

References (21)

1. Bao L, Locovei S, and Dahl G (2004) Pannexin membrane channels are mechanosensitive conduits for ATP. FEBS Lett 572:65-68.
2. Coutinho-Silva R and Persechini PM (1997) P2Z purinoceptor-associated pores induced by extracellular ATP in macrophages and J774 cells. Am J Physiol 273: C1793-C1800.
3. Donnelly-Roberts DL and Jarvis MF (2007) Discovery of P2X7 receptor-selective antagonists offers new insights into P2X7 receptor function and indicates a role in chronic pain states. Br J Pharmacol 151:571-579.
4. Faria RX, Defarias FP, and Alves LA (2005) Are second messengers crucial for opening the pore associated with P2X7 receptor? Am J Physiol Cell Physiol 288: C260-C271.
5. 2+. Am J Physiol Cell Physiol 297:C28-C42.
6. Jiang LH, Rassendren F, Mackenzie A, Zhang YH, Surprenant A, and North RA (2005) N-methyl-D-glucamine and propidium dyes use different permeation pathways at rat P2X(7) receptors. Am J Physiol Cell Physiol 289:C1295-C1302.
7. Khakh BS, Bao XR, Labarca C, and Lester HA (1999) Neuronal P2X transmittergated cation channels change their ion selectivity in seconds. Nat Neurosci 2:322-330.
8. Le Stunff H and Raymond MN (2007) P2X7 receptor-mediated phosphatidic acid production delays ATP-induced pore opening and cytolysis of RAW 264.7 macrophages. Cell Signal 19:1909-1918.
9. Locovei S, Bao L, and Dahl G (2006) Pannexin 1 in erythrocytes: function without a gap. Proc Natl Acad Sci U S A 103:7655-7659.
10. Locovei S, Scemes E, Qiu F, Spray DC, and Dahl G (2007) Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex. FEBS Lett 581:483-488.
11. North RA (2002) Molecular physiology of P2X receptors. Physiol Rev 82:1013-1067.
12. Pelegrin P and Surprenant A (2006) Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor. EMBO J 25:5071-5082.
13. Pelegrin P and Surprenant A (2007) Pannexin-1 couples to maitotoxin- and nigericin-induced interleukin-1beta release through a dye uptake-independent pathway. J Biol Chem 282:2386-2394.
14. Ralevic V and Burnstock G (1998) Receptors for purines and pyrimidines. Pharmacol Rev 50:413-492.
15. Schachter J, Motta AP, de Souza Zamorano A, da Silva-Souza HA, Guimarães MZ, and Persechini PM (2008) ATP-induced P2X7-associated uptake of large molecules involves distinct mechanisms for cations and anions in macrophages. J Cell Sci 121:3261-3270.
16. Schilling WP, Wasylyna T, Dubyak GR, Humphreys BD, and Sinkins WG (1999) Maitotoxin and P2Z/P2X(7) purinergic receptor stimulation activate a common cytolytic pore. Am J Physiol 277:C766-C776.
17. Steinberg TH, Newman AS, Swanson JA, and Silverstein SC (1987) ATP4- permeabilizes the plasma membrane of mouse macrophages to fluorescent dyes. J Biol Chem 262:8884-8888.
18. Surprenant A, Rassendren F, Kawashima E, North RA, and Buell G (1996) The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 272:735-738.
19. Virginio C, Church D, North RA, and Surprenant A (1997) Effects of divalent cations, protons and calmidazolium at the rat P2X7 receptor. Neuropharmacology 36: 1285-1294.
20. Virginio C, MacKenzie A, North RA, and Surprenant A (1999) Kinetics of cell lysis, dye uptake and permeability changes in cells expressing the rat P2X7 receptor. J Physiol 519:335-346.
21. Yan Z, Li S, Liang Z, Tomić M, and Stojilkovic SS (2008) The P2X7 receptor channel pore dilates under physiological ion conditions. J Gen Physiol 132:563-573.