α-ketoglutarate controls the ability of the Escherichia coli PII signal transduction protein to regulate the activities of NRII (NtrB) but does not control the binding of PII to NRII

Biochemistry

Volumn 48, Issue 48, 2009, Pages 11514-11521

  Jiang, Peng ^a   Ninfa, Alexander J ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLYLTRANSFERASE; AUTOPHOSPHORYLATION; CONCENTRATION OF; GLUTAMINE SYNTHETASE; KETOGLUTARATE; METABOLIC ENZYMES; NITROGEN METABOLISM; PHOSPHATASE ACTIVITY; PHYSIOLOGICAL CONCENTRATIONS; PHYSIOLOGICAL RANGE; SIGNALING PROTEINS; SIMILAR PATTERN; TWO COMPONENT SYSTEMS;

AMINO ACIDS; BACTERIOPHAGES; BINDING ENERGY; ESCHERICHIA COLI; METABOLISM; SIGNAL TRANSDUCTION;

PROTEINS;

2 OXOGLUTARIC ACID; BACTERIAL PROTEIN; PHOSPHATASE; PROTEIN PII; SIGNAL TRANSDUCTION PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; AUTOPHOSPHORYLATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; BINDING SITES; ENZYME ACTIVATION; ESCHERICHIA COLI PROTEINS; KETOGLUTARIC ACIDS; NUCLEOTIDYLTRANSFERASES; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHORYLATION; PII NITROGEN REGULATORY PROTEINS; PROTEIN KINASES; SIGNAL TRANSDUCTION;

ESCHERICHIA COLI;

EID: 73149096051     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi901158h     Document Type: Article

References (28)

1. Ninfa, A. J., and Atkinson, M. R. (2000) PII signal transduction proteins. Trends Microbiol. 8, 172-179.
2. Ninfa, A. J., and Jiang, P. (2005) PII signal transduction proteins: Sensors of α-ketoglutarate that regulate nitrogen metabolism. Curr. Opin. Microbiol. 8, 168-173.
3. Ninfa, A. J., Jiang, P., Atkinson, M. R., and Peliska, J. A. (2000) Integration of antagonistic signals in the regulation of bacterial nitrogen assimilation. Curr. Top. Cell. Regul. 36, 32-76.
4. Jiang, P., and Ninfa, A. J. (2007) The Escherichia coli PII signal transduction protein controlling nitrogen assimilation acts as a sensor of adenylate energy charge in vitro. Biochemistry 46, 12976-12986.
5. Jiang, P., Peliska, J., and Ninfa, A. J. (1998) Reconstitution of the signal transduction bicyclic cascade responsible for the regulation of Ntr gene transcription in Escherichia coli. Biochemistry 37, 12795-12801.
6. Stock, J. B., Ninfa, A. J., and Stock, A. M. (1989) Protein phosphorylation and the regulation of adaptive responses in bacteria. Microbiol. Rev. 53, 450-490.
7. Ninfa, A. J., Reitzer, L. J., and Magasanik, B. (1987) Initiation of transcription at the bacterial glnAp2 promoter by purified Escherichia coli components is facilitated by enhancers. Cell 50, 1039-1046.
8. Popham, D. L., Szeto, D., Keener, J., and Kustu, S. (1989) Function of a bacterial activator protein that binds to transcriptional enhancers. Science 243, 629-635.
9. Su, W., Porter, S., Kustu, S., and Echols, H. (1990) DNA-looping and enhancer activity: Association between DNA-bound NtrC activator and RNA polymerase at the bacterial glnA promoter. Proc. Natl. Acad. Sci. U.S.A. 87, 5504-5508.
10. Pioszak, A. A., and Ninfa, A. J. (2003) How the domains of NRII collaborate in the PII-activated phosphatase activity of Escherichia coli NRII (NtrB). Biochemistry 42, 8885-8899.
11. Keener, J., and Kustu, S. (1988) Protein kinase and phosphoprotein phosphatase activities of nitrogen regulatory proteins NTRB and NTRC of enteric bacteria: Roles of the conserved amino-terminal domain of NTRC. Proc. Natl. Acad. Sci. U.S.A. 85, 4976-4980.
12. Weiss, V., and Magasanik, B. (1988) Phosphorylation of Nitrogen Regulator I of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 85, 8919-8923.
13. Ninfa, A. J., and Bennett, R. L. (1991) Identification of the Site of Autophosphorylation of the Bacterial Protein Kinase/Phosphatase NRII. J. Biol. Chem. 266, 6888-6893.
14. Sanders, D. A., Gillece-Castro, B. L., Burlingame, A. L., and Koshland, D. E., Jr. (1992) Phosphorylation site of NtrC, a protein phosphatase whose covalent intermediate activates transcription. J. Bacteriol. 174, 5117-5122.
15. II, regulates the transcription of the glnALG operon in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 83, 5909-5913.
16. Pioszak, A. A., and Ninfa, A. J. (2003) Genetic and biochemical analysis of the PII-dependent phosphatase activity of Escherichia coli NRII. J. Bacteriol. 185, 1299-1315.
17. Jiang, P., and Ninfa, A. J. (1999) Regulation of the autophosphorylation of Escherichia coli NRII by the PII signal transduction protein. J. Bacteriol. 181, 1906-1911.
18. Kamberov, E. S., Atkinson, M. R., and Ninfa, A. J. (1995) The Escherichia coli PII signal transduction protein is activated upon binding 2-ketoglutarate and ATP. J. Biol. Chem. 270, 17797-17807.
19. Jiang, P., Peliska, J. A., and Ninfa, A. J. (1998) The regulation of glutamine synthetase covalent modification revisited: Role of 2-ketoglutarate in the regulation of glutamine synthetase adenylylation state. Biochemistry 37, 12802-12810.
20. Senior, P. J. (1975) Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: Studies with the continuous-culture technique. J. Bacteriol. 123, 407-418.
21. Atkinson, M. R., and Ninfa, A. J. (1998) Role of the glnK gene in nitrogen-regulation of gene transcription in Escherichia coli. Mol. Microbiol. 29, 431-447.
22. Jiang, P., Pioszak, A. A., and Ninfa, A. J. (2007) Structure/function analysis of glutamine synthetase adenylyltransferase (ATase, EC 2.7.7.49) of Escherichia coli. Biochemistry 46, 4117-4132.
23. Jiang, P., Mayo, A. E., and Ninfa, A. J. (2007) Escherichia coli glutamine synthetase adenylyltransferase (ATase, EC 2.7.7.49): Kinetic characterization of regulation by PII, PII-UMP, glutamine, and α-ketoglutarate. Biochemistry 46, 4133-4146.
24. Pioszak, A. A., Jiang, P., and Ninfa, A. J. (2000) The Escherichia coli PII signal transduction protein regulates the activities of the two-component system transmitter protein NRII by direct interaction with the kinase domain of the transmitter module. Biochemistry 39, 13450-13461.
25. Jiang, P., Atkinson, M. R., Srisawat, C., Sun, Q., and Ninfa, A. J. (2000) Functional dissection of the dimerization and enzymatic activities of Escherichia coli nitrogen regulator II and their regulation by the PII protein. Biochemistry 39, 13433-13449.
26. Jiang, P., and Ninfa, A. J. (2000) Asymmetry in the autophosphorylation of the two-component system transmitter protein NRII (NtrB) of Escherichia coli. Biochemistry 39, 5058-5065.
27. Jiang, P., Zucker, P., Atkinson, M. R., Kamberov, E. S., Tirasophon, W., Chandran, P., Schefke, B. R., and Ninfa, A. J. (1997) Structure/function analysis of the PII signal transduction protein of Escherichia coli: Genetic separation of interactions with protein receptors. J. Bacteriol. 179, 4342-4353.
28. McClure, W. R. (1985) Mechanism and control of transcription initiation in prokaryotes. Annu. Rev. Biochem. 54, 171-204.