An interdomain disulfide bridge links the NtA and first FS domain in agrin

Protein Science

Volumn 18, Issue 12, 2009, Pages 2421-2428

  McFarlane, Ainsley A ^a   Stetefeld, Jörg ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Agrin; Follistatin; FOLN subdomain; Interdomain disulfide; Kazal subdomain; N terminal agrin

Indexed keywords

AGRIN; CYSTEINE; FOLLISTATIN; LAMININ; LEUCINE; VALINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; DISULFIDE BOND; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE ALIGNMENT;

AGRIN; AMINO ACID SEQUENCE; ANIMALS; CHICKENS; CRYSTALLOGRAPHY, X-RAY; DISULFIDES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 73149084216     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.276     Document Type: Article

References (26)

1. Hohenester E, Engel J (2002) Domain structure and organisation in extracellular matrix proteins. Matrix Biol 21:115-128.
2. Bork P, Downing AK, Kieffer B, Campbell ID (1996) Structure and distribution of modules in extracellular proteins. Q Rev Biophys 29:119-167.
3. Ruegg MA, Bixby JL (1998) Agrin orchestrates synaptic differentiation at the vertebrate neuromuscular junction. Trends Neurosci 21:22-27.
4. Stetefeld J (2001) The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat Struct Biol 8: 705-709.
5. Stetefeld J, Ruegg MA (2005) Structural and functional diversity generated by alternative mRNA splicing. Trends Biochem Sci 30:515-521.
6. Denzer AJ, Gesemann M, Schumacher B, Ruegg MA (1995) An amino-terminal extension is required for the secretion of chick agrin and its binding to extracellular matrix. J Cell Biol 131:1547-1560.
7. Denzer AJ, Hauser DM, Gesemann M, Ruegg MA (1997) Synaptic differentiation: The role of agrin in the formation and maintenance of the neuromuscular junction. Cell Tissue Res 290:357-365.
8. Mascarenhas J (2003) Mapping of the laminin-binding site of the N-terminal agrin domain (NtA). Embo J 22: 529-536.
9. Gesemann M (1996) Alternative splicing of agrin alters its binding to heparin, dystroglycan, and the putative agrin receptor. Neuron 16:755-767.
10. Hohenester E, Maurer P, Timpl R (1997) Crystal structure of a pair of follistatin-like and EF-hand calcium-binding domains in BM-40. Embo J 16:3778-3786.
11. van de Locht A (1995) Two heads are better than one: Crystal structure of the insect derived double domain Kazal inhibitor rhodniin in complex with thrombin. Embo J 14:5149-5157.
12. Cole AR (1999) Disulfide bond structure and N-glycosylation sites of the extracellular domain of the human interleukin-6 receptor. J Biol Chem 274:7207-7215.
13. Muralidhara BK, Hirose M (2000) Structural and functional consequences of removal of the interdomain disulfide bridge from the isolated C-lobe of ovotransferrin. Protein Sci 9:1567-1575.
14. Mascarenhas JB (2005) Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells. Matrix Biol 23:507-513.
15. Bork P, Holm L, Sander C (1994) The immunoglobulin fold. Structural classification, sequence patterns and common core. J Mol Biol 242:309-320.
16. Huber AH, Wang YM, Bieber AJ, Bjorkman PJ (1994) Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2.0 Å. Neuron 12:717-731.
17. Innis CA, Hyvonen M (2003) Crystal structures of the heparan sulphate-binding domain of follistatin: Insights into ligand binding. J Biol Chem 278:39969-39977.
18. Nagase H, Woessner JF (1999) Matrix metalloproteinases. J Biol Chem 274:21491-21494.
19. Werle MJ, VanSaun M (2003) Activity dependent removal of agrin from synaptic basal lamina by matrix metalloproteinase 3. J Neurocytol 32:905-913.
20. Smith MA, Hilgenberg LG (2002) Agrin in the CNS: A protein in search of a function? Neuroreport 13: 1485-1495.
21. Biroc SL, Payan DG, Fisher JM (1993) Isoforms of agrin are widely expressed in the developing rat and may function as protease inhibitors. Brain Res Dev Brain Res 75: 119-129.
22. Tsen G, Halfter W, Kroger S, Cole GJ (1995) Agrin is a heparan sulfate proteoglycan. J Biol Chem 270:3392-3399.
23. Ozbek S, Muller J, Figgemeier E, Stetefeld J (2005) Favourable mediation of crystal contacts by cocoamido-propylbetaine (CAPB). Acta Crystallogr D Biol Crystallogr 61:477-480.
24. CCP4, Collaborative Computing Project No. 4 (1994) The CCP4 suite: Programs for protein crystallography. Acta Cryst D50:760-763.
25. Brunger AT (1998) Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 54:905-921.
26. Gabb HA, Jackson RM, Sternberg, M (1997) Modelling protein docking using shape complementarity, electrostatics and biochemical information. J Mol Biol 272: 106-120.