Oligomerization of Cry11Aa from Bacillus thuringiensis has an important role in toxicity against Aedes aegypti

Applied and Environmental Microbiology

Volumn 75, Issue 23, 2009, Pages 7548-7550

  Muñoz Garay, Carlos ^a   Rodríguez Almazán, Claudia ^a   Aguilar, Jose N ^a   Portugal, Leivi ^a   Gómez, Isabel ^a   Saab Rincon, Gloria ^a   Soberón, Mario ^a   Bravo, Alejandra ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

AEDES AEGYPTI; BACILLUS THURINGIENSIS; CRY11AA; INSECTICIDAL ACTIVITY; MEMBRANE RECEPTORS;

BACTERIOLOGY; TOXICITY;

OLIGOMERIZATION;

BACILLUS THURINGIENSIS TOXIN; CRY11AA TOXIN; CYT1AA TOXIN; UNCLASSIFIED DRUG;

BACTERIUM; BIOCONTROL AGENT; INSECTICIDE; MOSQUITO; MUTATION; SYNERGISM; TOXIC SUBSTANCE; TOXICITY;

AEDES AEGYPTI; ALPHA HELIX; ARTICLE; BACILLUS THURINGIENSIS; BACTERIUM MUTANT; DRUG ACTIVITY; INSECTICIDAL ACTIVITY; MUTANT; NONHUMAN; OLIGOMERIZATION; TOXICITY;

AEDES; ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; ENDOTOXINS; HEMOLYSIN PROTEINS; PROTEIN MULTIMERIZATION; SEQUENCE DELETION;

AEDES AEGYPTI; BACILLUS THURINGIENSIS;

EID: 72949100332     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01303-09     Document Type: Article

References (16)

1. Chang, C., Y. M. Yu, S. M. Dai, S. K. Law, and S. S. Gill. 1993. High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes. Appl. Environ. Microbiol. 59:815-821.
2. Compton, L. A., and W. C. Johnson, Jr. 1986. Analysis of protein circular dichroism spectra for secondary structure using a simple matrix multiplication. Anal. Biochem. 155:155-167.
3. Crickmore, N., E. J. Bone, J. A. Williams, and D. J. Ellar. 1995. Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis subsp israelensis. FEMS Microbiol. Lett. 131:249-254.
4. Gómez, I., J. Sánchez, R. Miranda, A. Bravo, and M. Soberón. 2002. Cadherin-like receptor binding facilitates proteolytic cleavage of helix α-1 in domain I and oligomer pre-pore formation of Bacillus thuringiensis Cry1Ab toxin. FEBS Lett. 513:242-246.
5. Jiménez-Juárez, N., C. Muñoz-Garay, I. Gómez, G. Saab-Rincon, J. Y. Damian-Alamazo, S. S. Gill, M. Soberón, and A. Bravo. 2007. Bacillus thuringiensis Cry1Ab mutants affecting oligomer formation are non toxic to Manduca sexta larvae. J. Biol. Chem. 282:21222-21229.
6. Lereclus, D., H. Agaisse, M. Gominet, and J. Chaufaux. 1995. Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spoOA mutant. Bio/Technology 13:67-71.
7. Lupas, A., M. Van Dyke, and J. Stock. 1991. Predicting coiled coils from protein sequences. Science 252:1162-1164. (Pubitemid 21917026)
8. Pérez, C., L. E. Fernandez, J. Sun, J. L. Folch, S. S. Gill, M. Soberón, and A. Bravo. 2005. Bti Cry11Aa and Cyt1Aa toxins interactions support the synergism-model that Cyt1Aa functions as membrane-bound receptor. Proc. Natl. Acad. Sci. USA 102:18303-18308.
9. Pérez, C., C. Muñoz-Garay, L. C. Portugal, J. Sánchez, S. S. Gill, M. Soberón, and A. Bravo. 2007. Bacillus thuringiensis subsp. israelensis Cyt1Aa enhances activity of Cry11Aa toxin by facilitating the formation of a pre-pore oligomeric structure. Cell. Microbiol. 9:2931-2937.
10. Rausell, C., L. Pardo-López, J. Sánchez, C. Muñoz-Garay, C. Morera, M. Soberón, and A. Bravo. 2004. Unfolding events in the water-soluble monomeric Cry1Ab toxin during transition to oligomeric pre-pore and membrane inserted pore channel. J. Biol. Chem. 279:55168-55175.
11. Schnepf, E., N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:775-806.
12. Soberón, M., L. E. Fernández, C. Pérez, S. S. Gill, and A. Bravo. 2007. Mode of action of mosquitocidal Bacillus thuringiensis toxins. Toxicon 49:597-600.
13. Sreerama, N., and R. W. Woody. 2000. Estimation of protein secondary structure from CD spectra: comparison of CONTIN, SELCON and CDSSTR methods with an expanded reference set. Anal. Biochem. 287:252-260.
14. Wirth, M. C., G. P. Georghiou, and B. A. Federici. 1997. CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus. Proc. Natl. Acad. Sci. USA 94:10536-10540.
15. Wirth, M. C., W. E. Walton, and B. A. Federici. 2000. Cyt1Aa from Bacillus thuringiensis restores toxicity of Bacillus sphaericus against resistant Culex quinquefasciatus (Diptera:Culicidae). J. Med. Entomol. 37:401-407.
16. Wu, D., J. J. Johnson, and B. A. Federici. 1994. Synergism of mosquitocidal toxicity between CytA and CryIV proteins using inclusions produced from cloned genes of Bacillus thuringiensis subsp. israelensis. Mol. Microbiol. 13:965-972. (Pubitemid 24985882)