Robotic Liquid Handlers and Semiautomated Cell Quantification Systems Increase Consistency and Reproducibility in High-Throughput, Cell-Based Assay

Journal of Laboratory Automation

Volumn 15, Issue 1, 2010, Pages 7-14

  Heinzman, Jamie M ^a   Rice, Shara D ^a   Corkan, L A ^a  

^a Precision Therapeutics Inc   (United States)

Author keywords

automation; cell quantification; cell based assay; chemosensitivity and resistance assay; liquid handling

Indexed keywords

ACCURACY AND PRECISION; ASSAY LIQUID; AUTOMATED SYSTEMS; AUTOMATION CELL; CELL COUNT; CELL-BASED ASSAYS; CHEMOSENSITIVITY; CHEMOTHERAPEUTIC AGENTS; CLINICAL UTILITY; COMPUTERIZED TOOLS; COUNTING SYSTEM; HANDLING SYSTEMS; HIGH-THROUGHPUT; PITTSBURGH; REPRODUCIBILITIES; ROBOTIC LIQUID HANDLER; SEMI-AUTOMATED; TUMOR CELLS;

ASSAYS; AUTOMATION; CELLS; CYTOLOGY; LIQUIDS;

BIOINFORMATICS;

CISPLATIN; DOXORUBICIN; PACLITAXEL;

ACCURACY; ARTICLE; AUTOMATION; CANCER RESISTANCE; CELL ASSAY; CELL COUNT; CHEMOSENSITIVITY; COLON CANCER; CONTROLLED STUDY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LUNG CANCER; OVARY CANCER; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; ROBOTICS;

EID: 72649091079     PISSN: 22110682     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2009.08.010     Document Type: Article

References (10)

1. Bellamy W. T. Prediction of response to drug therapy of cancer. A review of in vitro assays. Drugs 1992, 44, 690–708.
2. Kurbacher C. M. Cree I. A. Chemosensitivity testing using microplate adenosine triphosphate-based luminescence measurements In In Vitro Assays; Blumenthal R. D., Ed. Humana Press, Inc: Totowa, NJ; 2005; pp 101–120.
3. Burton J. D. The MTT assay to evaluate chemosensitivity In In Vitro Assays; Humana Press: Totowa, NJ; 2005; pp 69–78.
4. Scudiero D. A. Shoemaker R. H. Paull K. D. Monks A. Tierney S. Nofziger T. H. Currens M. J. Seniff D. Boyd M. R. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 1988, 48, 4827–4833.
5. In vitro diagnostic multivariate index assays. Draft guidance for industry, clinical laboratories, and FDA staff. 2007, Docket number 2006D-0347.
6. Kornblith P. Wells A. Gabrin M. J. Piwowar J. Chattopadhyay A. George L. D. Ochs R. L. Burholt D. In vitro responses of ovarian cancers to platinums and taxanes. Anticancer Res. 2003, 23, 543–548.
7. Ochs R. L. Fensterer J. Ohori N. P. Wells A. Gabrin M. George L. D. Kornblith P. Evidence for the isolation, growth, and characterization of malignant cells in primary cultures of human tumors. In Vitro Cell Dev. Biol. Anim. 2003, 39, 63–70.
8. Ochs R. L. Burholt D. Kornblith P. The ChemoFx assay: An ex vivo cell culture assay for predicting anticancer drug responses In Chemosensitivity; Blumenthal R., Ed. Humana Press: Totowa, NJ; 2004; pp 155–172.
9. Mi Z. Holmes F. A. Hellerstedt B. Pippen J. Collea R. Backner A. Bush J. E. Gallion H. H Wells A. O'Shaughnessy J. A. Feasibility assessment of a chemoresponse assay to predict pathologic response in neoadjuvant chemotherapy for breast cancer patients. Anticancer Res. 2008, 28, 1733–1740.
10. Gallion H. Christopherson W. A. Coleman R. L. DeMars L. Herzog T. Hosford S. Schellhas H. Wells A. Sevin B. U. Progression-free interval in ovarian cancer and predictive value of an ex vivo chemoresponse assay. Int. J. Gynecol. Cancer 2006, 16, 194–201.