Substrate specificity of microbial transglutaminase as revealed by three-dimensional docking simulation and mutagenesis

Protein Engineering, Design and Selection

Volumn 22, Issue 12, 2009, Pages 747-752

  Tagami, Uno ^a   Shimba, Nobuhisa ^a   Nakamura, Mina ^a   Yokoyama, Kei Ichi ^a   Suzuki, Ei Ichiro ^a   Hirokawa, Takatsugu ^b  

^a AJINOMOTO CO INC   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Molecular dynamics; Site directed mutagenesis; Substrate docking; Substrate specificity; Transglutaminase

Indexed keywords

ACTIVE SITE; AMIDE GROUPS; AROMATIC RESIDUES; DOCKING MODELS; DOCKING SIMULATIONS; IN-FIELD; MICROBIAL TRANSGLUTAMINASE; OXYANIONS; PEPTIDE SUBSTRATES; SITE DIRECTED MUTAGENESIS; SUBSTRATE SPECIFICITY; TRANSGLUTAMINASES;

AMIDES; AMINES; BINDING ENERGY; CALCIUM; DOCKING; FLUORINE CONTAINING POLYMERS; GAS GENERATORS; HYDROGELS; MOLECULAR DYNAMICS; MUTAGENESIS; PLANTS (BOTANY); SIMULATORS; SUBSTRATES; THICKNESS GAGES; THREE DIMENSIONAL;

BINDING SITES;

ALANINE; GLUTAMINASE;

ARTICLE; BINDING SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SPECIFICITY; HYDROPHOBICITY; MUTAGENESIS; PRIORITY JOURNAL;

MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; TRANSAMINASES;

EID: 72649084947     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp061     Document Type: Article

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