Targeted transfection increases siRNA uptake and gene silencing of primary endothelial cells in vitro - A quantitative study

Journal of Controlled Release

Volumn 141, Issue 2, 2010, Pages 241-251

  Ásgeirsdóttir, Sigridur A ^a   Talman, Eduard G ^b   de Graaf, Inge A ^c   Kamps, Jan A A M ^a   Satchell, Simon C ^d   Mathieson, Peter W ^d   Ruiters, Marcel H J ^a,b   Molema, Grietje ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^b Synvolux Therapeutics Inc   (Netherlands)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

^d UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Cationic lipid; E selectin; Microvascular endothelial cells; SAINT; Targeting

Indexed keywords

AMPHIPHILICS; ANTIGEN RECOGNITION; CARRIER SYSTEMS; CATIONIC LIPIDS; CELL TYPES; ENHANCED UPTAKE; EX-VIVO; GENE SILENCING; HUMAN KIDNEY TISSUE; HUMAN MICROVASCULAR ENDOTHELIAL CELLS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; IN-VITRO; INFLAMMATORY CELLS; INFLAMMATORY CONDITIONS; LIPOPLEXES; MICROVASCULAR ENDOTHELIAL CELLS; NON-TARGETED; NONVIRAL; PROTEIN EXPRESSIONS; QUANTITATIVE STUDY; SELECTINS; SIRNA DELIVERY; SMALL INTERFERING RNA (SIRNA); SPECIFIC CAPACITIES; TARGETING SYSTEMS; VE-CADHERIN;

ADHESION; ANTIBODIES; BLOOD VESSEL PROSTHESES; CELL ADHESION; GENETIC ENGINEERING; NUCLEIC ACIDS; RNA; SELF ASSEMBLY;

ENDOTHELIAL CELLS;

1 METHYL 4 (9 DIOLELYL)METHYLPYRIDINIUM CHLORIDE; AMPHOPHILE; CD31 ANTIGEN; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; LIPOPLEX; SMALL INTERFERING RNA; TUMOR NECROSIS FACTOR RECEPTOR 2; UNCLASSIFIED DRUG; VASCULAR ENDOTHELIAL CADHERIN;

ADULT; AGED; ANTIGEN RECOGNITION; ARTICLE; CELL ADHESION; CELL SPECIFICITY; CONDITIONALLY IMMORTALIZED GLOMERULAR ENDOTHELIAL CELL; CONTROLLED STUDY; ENDOTHELIUM CELL; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN MICROVASCULAR ENDOTHELIAL CELL; HUMAN TISSUE; HUMAN UMBILICAL VEIN ENDOTHELIAL CELL; IN VITRO STUDY; LIPOSOMAL GENE DELIVERY SYSTEM; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE STUDY; ZETA POTENTIAL;

AGED; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; BINDING SITES; BIOLOGICAL TRANSPORT; CADHERINS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; ENDOTHELIAL CELLS; HUMANS; INFLAMMATION; KIDNEY; MALE; MIDDLE AGED; PARTICLE SIZE; PYRIDINIUM COMPOUNDS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TISSUE CULTURE TECHNIQUES; TRANSFECTION;

EID: 72449163929     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jconrel.2009.09.008     Document Type: Article

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