Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants

Journal of Bacteriology

Volumn 191, Issue 24, 2009, Pages 7581-7586

  Koziol, Uriel ^a   Hannibal, Luciana ^a,c   Rodríguez, María Cecilia ^a   Fabiano, Elena ^a   Kahn, Michael L ^b   Noya, Francisco ^a  

^a UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^b WASHINGTON STATE UNIVERSITY   (United States)

^c LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACONITATE HYDRATASE; CITRATE SYNTHASE; IRON; ISOCITRATE DEHYDROGENASE;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL SURVIVAL; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE DELETION; GENE FUNCTION; GENE MUTATION; MUTAGENESIS; NODULATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; SINORHIZOBIUM MELILOTI;

ACONITATE HYDRATASE; BACTERIAL PROTEINS; CITRATE (SI)-SYNTHASE; CITRATES; GENE DELETION; HISTOCYTOCHEMISTRY; MICROBIAL VIABILITY; ROOT NODULES, PLANT; SINORHIZOBIUM MELILOTI;

BACTERIA (MICROORGANISMS); SINORHIZOBIUM MELILOTI 1021;

EID: 72449149315     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00777-09     Document Type: Article

References (44)

1. Alen, C., and A. L. Sonenshein. 1999. Bacillus subtilis aconitase is an RNA-binding protein. Proc. Natl. Acad. Sci. USA 96:10412-10417.
2. Banerjee, S., A. K. Nandyala, P. Raviprasad, N. Ahmed, and S. E. Hasnain. 2007. Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase. J. Bacteriol. 189:4046-4052.
3. Beinert, H., M. C. Kennedy, and C. D. Stout. 1996. Aconitase as iron-sulfur protein, enzyme, and iron-regulatory protein. Chem. Rev. 96:2335-2374.
4. Beringer, J. E. 1974. R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84:188-198.
5. Cowie, A., J. Cheng, C. D. Sibley, Y. Fong, R. Zaheer, C. L. Patten, R. M. Morton, G. B. Golding, and T. M. Finan. 2006. An integrated approach to functional genomics: construction of a novel reporter gene fusion library for Sinorhizobium meliloti. Appl. Environ. Microbiol. 72:7156-7167. (Pubitemid 44748429)
6. Craig, J. E., M. J. Ford, D. C. Blaydon, and A. L. Sonenshein. 1997. A null mutation in the Bacillus subtilis aconitase gene causes a block in Spo0A-phosphate- dependent gene expression. J. Bacteriol. 179:7351-7359.
7. de Lorenzo, V., L. Eltis, B. Kessler, and K. N. Timmis. 1993. Analysis of Pseudomonas gene products using lacIq/Ptrp-lac plasmids and transposons that confer conditional phenotypes. Gene 123:17-24. (Pubitemid 23035786)
8. Driscoll, B. T., and T. M. Finan. 1993. NAD(+)-dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation. Mol. Microbiol. 7:865-873.
9. +-dependent malic enzyme of Rhizobium meliloti. J. Bacteriol. 178:2224-2231.
10. Duncan, M. J., and D. G. Fraenkel. 1979. α-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti. J. Bacteriol. 137:415-419.
11. Dymov, S. L., D. J. J. Meek, B. Steven, and B. T. Driscoll. 2004. Insertion of transposon Tn5tac1 in the Sinorhizobium meliloti malate dehydrogenase (mdh) gene results in conditional polar effects on downstream TCA cycle genes. Mol. Plant-Microbe Interact. 17:1318-1327.
12. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
13. Galibert, F., T. M. Finan, S. R. Long, A. Puhler, P. Abola, F. Ampe, F. Barloy-Hubler, M. J. Barnett, A. Becker, P. Boistard, G. Bothe, M. Boutry, L. Bowser, J. Buhrmester, E. Cadieu, D. Capela, P. Chain, A. Cowie, R. W. Davis, S. Dreano, N. A. Federspiel, R. F. Fisher, S. Gloux, T. Godrie, A. Goffeau, B. Golding, J. Gouzy, M. Gurjal, I. Hernandez-Lucas, A. Hong, L. Huizar, R. W. Hyman, T. Jones, D. Kahn, M. L. Kahn, S. Kalman, D. H. Keating, E. Kiss, C. Komp, V. Lelaure, D. Masuy, C. Palm, M. C. Peck, T. M. Pohl, D. Portetelle, B. Purnelle, U. Ramsperger, R. Surzycki, P. Thebault, M. Vandenbol, F. J. Vorholter, S. Weidner, D. H. Wells, K. Wong, K. C. Yeh, and J. Batut. 2001. The composite genome of the legume symbiont Sinorhizobium meliloti. Science 293:668-672. (Pubitemid 32728664)
14. Gardiol, A., A. Arias, C. Cervenansky, and G. Martinez-Drets. 1982. Succinate dehydrogenase mutant of Rhizobium meliloti. J. Bacteriol. 151:1621-1623.
15. Gruer, M. J., A. J. Bradbury, and J. R. Guest. 1997. Construction and properties of aconitase mutants of Escherichia coli. Microbiology 143:1837-1846. (Pubitemid 27290272)
16. Grzemski, W., J. P. Akowski, and M. L. Kahn. 2005. Probing the Sinorhizobium meliloti-alfalfa symbiosis using temperature-sensitive and impairedfunction citrate synthase mutants. Mol. Plant-Microbe Interact. 18:134-141.
17. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
18. House, B. L., M. W. Mortimer, and M. L. Kahn. 2004. New recombination methods for Sinorhizobium meliloti genetics. Appl. Environ. Microbiol. 70: 2806-2815.
19. Humann, J. L., B. K. Schroeder, M. W. Mortimer, B. L. House, S. N. Yurgel, S. C. Maloney, K. L. Ward, H. M. Fallquist, H. T. Ziemkiewicz, and M. L. Kahn. 2008. Construction and expression of sugar kinase transcriptional gene fusions by using the Sinorhizobium meliloti ORFeome. Appl. Environ. Microbiol. 74:6756-6765.
20. Jefferson, R. A., T. A. Kavanagh, and M. W. Bevan. 1987. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901-3907.
21. Kaneko, T., Y. Nakamura, S. Sato, K. Minamisawa, T. Uchiumi, S. Sasamoto, A. Watanabe, K. Idesawa, M. Iriguchi, K. Kawashima, M. Kohara, M. Matsumoto, S. Shimpo, H. Tsuruoka, T. Wada, M. Yamada, and S. Tabata. 2002. Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110. DNA Res. 9:189-197. (Pubitemid 36307023)
22. Kennedy, M. C., M. H. Emptage, J. L. Dreyer, and H. Beinert. 1983. The role of iron in the activation-inactivation of aconitase. J. Biol. Chem. 258:11098-11105.
23. Kennedy, M. C., L. Mende-Mueller, G. A. Blondin, and H. Beinert. 1992. Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein. Proc. Natl. Acad. Sci. USA 89:11730-11734.
24. Lin, A. P., K. W. Hakala, S. T. Weintraub, and L. McAlister-Henn. 2008. Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase. Arch. Biochem. Biophys. 474:205-212.
25. McDermott, T. R., and M. L. Kahn. 1992. Cloning and mutagenesis of the Rhizobium meliloti isocitrate dehydrogenase gene. J. Bacteriol. 174:4790-4797.
26. Meade, H. M., S. R. Long, G. B. Ruvkun, S. E. Brown, and F. M. Ausubel. 1982. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J. Bacteriol. 149:114-122.
27. Mortimer, M. W., T. R. McDermott, G. M. York, G. C. Walker, and M. L. Kahn. 1999. Citrate synthase mutants of Sinorhizobium meliloti are ineffective and have altered cell surface polysaccharides. J. Bacteriol. 181:7608-7613.
28. Noya, F., A. Arias, and E. Fabiano. 1997. Heme compounds as iron sources for nonpathogenic Rhizobium bacteria. J. Bacteriol. 179:3076-3078.
29. Platero, R., L. Peixoto, M. R. O'Brian, and E. Fabiano. 2004. Fur is involved in manganese-dependent regulation of mntA (sitA) expression in Sinorhizobium meliloti. Appl. Environ. Microbiol. 70:4349-4355.
30. Poysti, N. J., E. D. Loewen, Z. Wang, and I. J. Oresnik. 2007. Sinorhizobium meliloti pSymB carries genes necessary for arabinose transport and catabolism. Microbiology 153:727-736.
31. Prodromou, C., M. J. Haynes, and J. R. Guest. 1991. The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn). J. Gen. Microbiol. 137:2505-2515.
32. Rose, I. A., and E. L. O'Connell. 1967. Mechanism of aconitase action. I. The hydrogen transfer reaction. J. Biol. Chem. 242:1870-1879.
33. Schafer, A., A. Tauch, W. Jager, J. Kalinowski, G. Thierbach, and A. Puhler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73. (Pubitemid 24224965)
34. Schroeder, B. K., B. L. House, M. W. Mortimer, S. N. Yurgel, S. C. Maloney, K. L. Ward, and M. L. Kahn. 2005. Development of a functional genomics platform for Sinorhizobium meliloti: construction of an ORFeome. Appl. Environ. Microbiol. 71:5858-5864.
35. Simon, R., J. Quandt, and W. Klipp. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in gram-negative bacteria. Gene 80:161-169. (Pubitemid 19192673)
36. Somerville, G., C. A. Mikoryak, and L. Reitzer. 1999. Physiological characterization of Pseudomonas aeruginosa during exotoxin A synthesis: glutamate, iron limitation, and aconitase activity. J. Bacteriol. 181:1072-1078.
37. Somerville, J. E., and M. L. Kahn. 1983. Cloning of the glutamine synthetase I gene from Rhizobium meliloti. J. Bacteriol. 156:168-176.
38. Tang, Y., and J. R. Guest. 1999. Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases. Microbiology 145:3069-3079. (Pubitemid 29535027)
39. Tang, Y., J. R. Guest, P. J. Artymiuk, R. C. Read, and J. Green. 2004. Post-transcriptional regulation of bacterial motility by aconitase proteins. Mol. Microbiol. 51:1817-1826.
40. Tang, Y., M. A. Quail, P. J. Artymiuk, J. R. Guest, and J. Green. 2002. Escherichia coli aconitases and oxidative stress: post-transcriptional regulation of sodA expression. Microbiology 148:1027-1037. (Pubitemid 34436778)
41. Thony-Meyer, L., and P. Kunzler. 1996. The Bradyrhizobium japonicum aconitase gene (acnA) is important for free-living growth but not for an effective root nodule symbiosis. J. Bacteriol. 178:6166-6172.
42. Uhrigshardt, H., M. Walden, H. John, and S. Anemuller. 2001. Purification and characterization of the first archaeal aconitase from the thermoacidophilic Sulfolobus acidocaldarius. Eur. J. Biochem. 268:1760-1771.
43. Viollier, P. H., K. T. Nguyen, W. Minas, M. Folcher, G. E. Dale, and C. J. Thompson. 2001. Roles of aconitase in growth, metabolism, and morphological differentiation of Streptomyces coelicolor. J. Bacteriol. 183:3193-3203.
44. Wilson, T. J., N. Bertrand, J. L. Tang, J. X. Feng, M. Q. Pan, C. E. Barber, J. M. Dow, and M. J. Daniels. 1998. The rpfA gene of Xanthomonas campestris pathovar campestris, which is involved in the regulation of pathogenicity factor production, encodes an aconitase. Mol. Microbiol. 28:961-970.