The large PAO cells in full-scale EBPR biomass samples are not yeast spores but possibly novel members of the β-Proteobacteria

Water Science and Technology

Volumn 50, Issue 6, 2004, Pages 123-130

  Chua, Adeline S M ^a   Eales, K ^b   Mino, T ^a   Seviour, R ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b LA TROBE UNIVERSITY   (Australia)

Author keywords

EBPR; FISH MAR; In situ physiology; PAO; Yeast spores; Proteobacteria

Indexed keywords

BIODIVERSITY; BIOMASS; CELLS; MICROORGANISMS; ORGANIC COMPOUNDS; PHYSIOLOGY;

ENHANCED BIOLOGICAL PHOSPHATE REMOVAL (EBPR); FLUORESCENT IN SITU HYBRIDIZATION (FISH); POLYPHOSPHATE ACCUMULATING ORGANISMS (PAO);

YEAST;

ACETIC ACID; ASPARTIC ACID; GLUCOSE; GLUTAMIC ACID; POLYHYDROXYALKANOIC ACID; POLYPHOSPHATE;

BACTERIUM; PHYSIOLOGY; YEAST;

AEROBIC METABOLISM; ANAEROBIC METABOLISM; ARTICLE; AUTORADIOGRAPHY; BACTERIAL CELL; BIODIVERSITY; BIOMASS; CLUSTER ANALYSIS; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGUS SPORE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; MICROORGANISM; NONHUMAN; SAMPLING; STAINING;

BETAPROTEOBACTERIA; NEGIBACTERIA; POSIBACTERIA; PROKARYOTA; PROTEOBACTERIA; RHODOCYCLUS; VICTORIA;

EID: 7244254570     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2004.0368     Document Type: Article

References (25)

1. Andreasen, K. and Nielsen, P.H. (1997). Application of Microautoradiography to the Study of Substrate Uptake by Filamentous Microorganisms in Activated Sludge. Appl. Environ. Microbiol., 63, 3662-3668.
2. Cech, J.S. and Hartman, P. (1990). Glucose induced breakdown of enhanced biological phosphate removal systems. Environ. Technol. 11, 651-656.
3. Cech, J.S. and Hartman, P. (1993). Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems. Wat. Res., 27, 1219-1225.
4. Crocetti, G.R., Hugenholtz, P., Bond, P.L., Schuler, A., Keller, J., Jenkins, D. and Blackall, L.L. (2000). Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Appl. Environ. Microbiol. 66(3), 1175-82.
5. Daims, H., Bruhl, A., Amann, R., Schleifer, K.H. and Wagner, M. (1999). The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol., 22(3), 434-44.
6. Fuhs, G.W. and Chen, M. (1975). Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microb. Ecol., 2, 119-138.
7. Hesselmann, R.P., Werlen, C., Hahn, D., van der Meer, J.R. and Zehnder, A.J. (1999). Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst. Appl. Microbiol., 22(3), 454-65.
8. Hicks, R.E., Amann, R.I. and Stahl, D.A. (1992). Dual staining of natural bacterioplancton with 4′ 6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences. Appl. Envir. Microbiol., 58, 2158-2163.
9. Ito, T., Nielsen, J.L., Okabe, S., Watanabe, Y. and Nielsen, P.H. (2002). Phylogenetic identification and substrate uptake patterns of sulfate-reducing bacteria inhabiting an oxic-anoxic sewer biofilm determined by combining microautoradiography and fluorescent in situ hybridization. Appl. Envir. Microbiol., 68, 356-364.
10. Jeon, C.O. and Park, J.M. (2000). Enhanced biological phosphorus removal in a sequencing batch reactor fed with glucose as a sole carbon source. Wat. Res., 34, 2160-2170.
11. Kawaharasaki, M., Tanaka, H., Kanagawa, T. and Nakamura, K. (1999). In situ identification of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4′,6-diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration. Wat. Res., 33(1), 257-265.
12. Kitamura, S. and Doi, Y. (1994). Staining method of poly(3-hydroxyalkanoic acids) producing bacteria by Nile Blue. Biotechnol. Tech., 8, 345-350.
13. Kong, Y.H., Beer, M., Rees, G.N. and Seviour, R.J. (2002). Functional analysis of microbial communities in aerobic-anaerobic sequencing batch reactors fed with different phosphorus/carbon (P/C) ratios. Microbiology, 148(8), 2299-307.
14. Lee, N., Nielsen, P.H., Andreasen, K.H., Juretschko, S., Nielsen, J.L., Schleifer, K.H. and Wagner, M. (1999). Combination of fluorescent in situ hybridization and microautoradiography - a new tool for structure-function analyses in microbial ecology. Appl. Envir. Microbiol., 65(3), 1289-97.
15. Lee, N., Jansen, J.C., Aspegren, H., Henze, M., Nielsen, P.H. and Wagner, M. (2002). Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal. Wat. Sci. Tech., 46(1-2), 163-70.
16. Manz, W., Amann, R., Ludwig, W., Wagner, M. and Schleifer, K.H. (1992). Phylogenetic oligonucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst. Appl. Microbiol., 15, 593-600.
17. Meier, H., Amann, R., Ludwig, W. and Schleifer, K.H. (1999). Specific oligonucleotide probes for in situ detection of a major group of Gram-positive bacteria with low DNA G+C content. Syst. Appl. Microbiol., 22, 186-196.
18. Melasniemi, H. and Hernesmaa, A. (2000). Yeast spores seem to be involved in biological phosphate removal: a microscopic in situ case study. Microbiology, 146(3), 701-7.
19. Neef, A., Witzenberger, R. and Kampfer, P. (1999). Detection of sphingomonads and in situ identification in activated sludge using 16SrRNA-targeted oligonucleotide probes. J. Ind. Microbiol. Biotechnol., 23, 261-267.
20. Roller, C., Wagner, M., Amann, R., Ludwig, W. and Schleifer, K.H. (1994). In situ probing of gram-positive bacteria with high DNA G + C content using 23S rRNA-targeted oligonucleotides. Microbiology, 140, 2849-2858.
21. Satoh, H., Mino, T. and Matsuo, T. (1998). Anaerobic uptake of glutamate and aspartate by enhanced biological phosphorus removal activated sludge. Wat. Sci. Tech., 37, 579-582.
22. Wagner, M. and Loy, A. (2002). Bacterial community composition and function in sewage treatment systems. Curr. Opin. Biotechnol., 3(3), 218-27. Review.
23. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K.H. (1994). Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Envir. Microbiol., 60, 792-800.
24. Wang, N., Peng, J. and Hill, G. (2002). Biochemical model of glucose induced enhanced biological phosphorus removal under anaerobic condition. Wat. Res., 36, 49-58.
25. Zilles, J.L., Hung, C.H. and Noguera, D.R. (2002). Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal. Wat. Sci. Tech., 46(1-2), 123-8.