Collagenase predigestion on paraffin sections enhances collagen immunohistochemical detection without distorting tissue morphology

Biotechnic and Histochemistry

Volumn 79, Issue 2, 2004, Pages 55-64

  D'Andrea, Michael R ^a  

^a JOHNSON AND JOHNSON PHARMACEUTICAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Collagen; Collagenase; Immunohistochemistry; Over digestion

Indexed keywords

COLLAGEN; COLLAGENASE; FORMALDEHYDE; PARAFFIN;

ACCURACY; ANALYTIC METHOD; ANTIBODY LABELING; ARTICLE; CONTROLLED STUDY; DISSOCIATION; ENZYMATIC DEGRADATION; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNODETECTION; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MORPHOLOGY; PRESERVATION; RELIABILITY; TISSUE FIXATION; TISSUE SECTION; TISSUE STRUCTURE;

CELL SIZE; CELLS, CULTURED; COLLAGEN; COLLAGENASES; HUMANS; IMMUNOHISTOCHEMISTRY; PARAFFIN EMBEDDING; STAINING AND LABELING;

EID: 7244247254     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/10520290410001728972     Document Type: Article

References (17)

1. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1989) The Molecular Biology of the Cell. 2nd ed. Garland Publishing, Inc, New York. pp. 808-815.
2. Barsky SH, Roa NC, Restrepo C, Liotta LA (1984) Immunohistochemical enhancement of basement membrane antigens by pepsin: applications in diagnostic pathology. Am. J. Clin. Pathol. 82: 191-194.
3. Barsky SH, Siegal G, Jannotta F, Liotta LA (1983) Loss of basement membrane components by invasive tumors, but not by their benign counterparts. Lab Invest. 49: 140-148.
4. D'Andrea MR (2003) Evidence linking autoimmunity to neuronal cell death in Alzheimer's disease. Brain Res. 982: 19-30.
5. D'Andrea MR, Reiser PA, Gumula NA, Hertzog BM, Andrade-Gordon P (2001) Application of triple-label immunohistochemistry to characterize inflammation in Alzheimer's disease brains. Biotech. & Histochem. 76: 97-106.
6. D'Andrea MR, Reiser PA, Polkovitch DA, Gumula NA, Belkowski S, Branchide B, Hertzog BM, Schmidheiser D, Belkowski S, Gastard M, Andrade-Gordon P (2003) The use of formic acid to embellish amyloid plaque detection in Alzheimer's disease tissues misguides key observations. Neurosci. Lett. 342: 114-118.
7. Farquharson C, Robins SP (1989) Immunolocalization of collagen types I and III in the arterial wall of the rat. Histochem. J. 21: 172-178.
8. Grimaud JA, Druguet M, Emonard H, Peyrol S, Barioz CM, Guerret S (1985) Collagen immunotyping. NATO ASI Series, Series A: Life Sciences 93: 487-514.
9. Higuchi I, Suehara M, Iwaki H, Nakagawa M, Arimura K, Osame M (2001) Collagen VI deficiency Ullrich's disease. Ann. Neurol. 49: 54.
10. Kallioinen M, Autio-Harmainen H, Dammert K, Risteli J, Risteli L (1984) Basement membrane laminin and type IV collagen in various benign and malignant adnexal tumors of the skin: An imnumohistochemical study. J. Invest. Derm. 83: 276-280.
11. Kasper M, Schuh D, Muller M (1994) Immunochemical localization of the β subunit of prolyl 4-hydroxylase in human alveolar epithelial cells. Acta Histochem. 96: 309-313.
12. Liotta LA (1986) Tumor invasion and metastasis-role of the extracellular matrix: Rhoads Memorial Award Lecture. Cancer Res. 46: 1-7.
13. Lowry A, Wilcox D, Masson EA, Williams PE (1997) Immunohistochemical methods for semiquantitative analysis of collagen content in human peripheral nerve. J. Anat. 191: 367-374.
14. Rucklidge GJ, Dean V (1987) Immunochemical techniques in the study of protein degradation with particular reference to collagen. Meth. Surv. Biochem. Anal. 17: 387-392.
15. Sano J, Ishida A, Ishizaki M, Sugisaki Y, Yamanaka N, Masugi Y (1984) Immunofluorescent visualization of type IV collagen in the glomerular capillary basement membrane after digestion with a microbial protease. Biomed. Res. 5: 387-392.
16. Shi S-R, Cote RJ, Taylor CR (1997) Antigen retrieval immunohistochemistry: past, present, and future. J. Histochem. Cytochem. 54: 327-343.
17. Taylor CR, Shi S-R, Chaiwun B, Young L, Imam SA, Cote RJ (1994) Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval technique. Human Pathol. 25: 263-270.