Constitutive expression of β-glucuronidase gene fused with stress-inducible promoter of the pathogenesis-related 1a protein gene of tobacco in transgenic Kalanchoe blossfeldiana

Acta Horticulturae

Volumn 454, Issue , 1998, Pages 373-376

  Aida, Ryutaro ^a   Shibata, Michio ^a  

^a FOOD RESEARCH INSTITUTE   (Japan)

Author keywords

Genetic transformation

Indexed keywords

AGROBACTERIUM; KALANCHOE; KALANCHOE BLOSSFELDIANA; LACTUCA; NICOTIANA; NICOTIANA DEBNEYI; NICOTIANA GLUTINOSA; NICOTIANA TABACUM; SOLANUM TUBEROSUM; TORENIA;

EID: 72249122181     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.1998.454.44     Document Type: Article

References (9)

1. Aida, R. and Shibata, M., 1995. Comparison of promoter-dependent expression of β-glucuronidase genes in transgenic Torenia plants. Acta Horticulturae 392: 219-225
2. Aida, R. and Shibata, M., 1996. Transformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgene silencing. Plant Sci. in press
3. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72: 248-254
4. Enomoto, S., Itoh, H., Ohshima, M., and Ohashi, Y., 1990. Induced expression of a chimeric gene construct in transgenic lettuce plants using tobacco pathogenesis-related protein gene promoter region. Plant Cell Rep. 9: 6-9
5. Ishige, T., Ohshima, M., and Ohashi, Y., 1991. Transformation of Japanese potato cultivars with the p-glucuronidase gene fused with the promoter of the pathogenesis-related la protein gene of tobacco. Plant Sci. 73: 167-174
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7. Kosugi, S., Ohashi, Y., Nakajima, K., and Arai, Y., 1990. An improved assay for β-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous p-glucuronidase activity. Plant Sci. 70: 133-140
8. Ohashi, Y., Ohshima, M., Itoh, H., Matsuoka, M., Watanabe, S., Murakami, T., and Hosokawa, D., 1992. Constitutive expression of stress-inducible genes, including pathogenesis-related 1 protein gene in a transgenic interspecific hybrid of Nicotiana glutinosa x Nicotiana debneyi. Plant Cell Physiol. 33: 177-187
9. Ohshima, M., Itoh, H., Matsuoka, M., Murakami T., and Ohashi, Y., 1990. Analysis of stress-induced or salicylic acid-induced expression of the pathogenesis-related la protein gene in transgenic tobacco. Plant Cell 2: 95-106