A family of transcriptional antitermination factors necessary for synthesis of the capsular polysaccharides of Bacteroides fragilis

Journal of Bacteriology

Volumn 191, Issue 23, 2009, Pages 7288-7295

  Chatzidaki Livanis, Maria ^a   Coyne, Michael J ^a   Comstock, Laurie E ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYSACCHARIDE;

ARTICLE; BACTEROIDES FRAGILIS; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; GENE DELETION; GENE LOCUS; HYBRID; MUTATIONAL ANALYSIS; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION; TRANSCRIPTION TERMINATION; UNTRANSLATED REGION;

AMINO ACID SEQUENCE; BACTERIAL CAPSULES; BACTERIAL PROTEINS; BACTEROIDES FRAGILIS; BLOTTING, NORTHERN; BLOTTING, WESTERN; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYSACCHARIDES, BACTERIAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION FACTORS;

BACTEROIDES FRAGILIS;

EID: 72249111108     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00500-09     Document Type: Article

References (27)

1. Artsimovitch, I., and R. Landick. 2002. The transcriptional regulator RfaH stimulates RNA chain synthesis after recruitment to elongation complexes by the exposed nontemplate DNA strand. Cell 109:193-203. (Pubitemid 34518258)
2. Bailey, M. J., C. Hughes, and V. Koronakis. 1997. RfaH and the ops element, components of a novel system controlling bacterial transcription elongation. Mol. Microbiol. 26:845-851.
3. Baumann, H., A. O. Tzianabos, J. R. Brisson, D. L. Kasper, and H. J. Jennings. 1992. Structural elucidation of two capsular polysaccharides from one strain of Bacteroides fragilis using high-resolution NMR spectroscopy. Biochemistry 31:4081-4089.
4. Belogurov, G. A., R. A. Mooney, V. Svetlov, R. Landick, and I. Artsimovitch. 2009. Functional specialization of transcription elongation factors. EMBO J. 28:112-122.
5. Belogurov, G. A., M. N. Vassylyeva, V. Svetlov, S. Klyuyev, N. V. Grishin, D. G. Vassylyev, and I. Artsimovitch. 2007. Structural basis for converting a general transcription factor into an operon-specific virulence regulator. Mol. Cell 26:117-129.
6. Burns, C. M., L. V. Richardson, and J. P. Richardson. 1998. Combinatorial effects of NusA and NusG on transcription elongation and Rho-dependent termination in Escherichia coli. J. Mol. Biol. 278:307-316.
7. Burova, E., and M. E. Gottesman. 1995. NusG overexpression inhibits Rho-dependent termination in Escherichia coli. Mol. Microbiol. 17:633-641.
8. Cardinale, C. J., R. S. Washburn, V. R. Tadigotla, L. M. Brown, M. E. Gottesman, and E. Nudler. 2008. Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli. Science 320:935-938.
9. Comstock, L. E., M. J. Coyne, A. O. Tzianabos, and D. L. Kasper. 1999. Interstrain variation of the polysaccharide B biosynthesis locus of Bacteroides fragilis: characterization of the region from strain 638R. J. Bacteriol. 181:6192-6196.
10. Comstock, L. E., M. J. Coyne, A. O. Tzianabos, A. Pantosti, A. B. Onderdonk, and D. L. Kasper. 1999. Analysis of a capsular polysaccharide biosynthesis locus of Bacteroides fragilis. Infect. Immun. 67:3525-3532.
11. Comstock, L. E., A. Pantosti, and D. L. Kasper. 2000. Genetic diversity of the capsular polysaccharide C biosynthesis region of Bacteroides fragilis. Infect. Immun. 68:6182-6188.
12. Coyne, M. J., W. Kalka-Moll, A. O. Tzianabos, D. L. Kasper, and L. E. Comstock. 2000. Bacteroides fragilis NCTC9343 produces at least three distinct capsular polysaccharides: cloning, characterization, and reassignment of polysaccharide B and C biosynthesis loci. Infect. Immun. 68:6176-6181.
13. Coyne, M. J., A. O. Tzianabos, B. C. Mallory, V. J. Carey, D. L. Kasper, and L. E. Comstock. 2001. Polysaccharide biosynthesis locus required for virulence of Bacteroides fragilis. Infect. Immun. 69:4342-4350.
14. Coyne, M. J., K. G. Weinacht, C. M. Krinos, and L. E. Comstock. 2003. Mpi recombinase globally modulates the surface architecture of a human commensal bacterium. Proc. Natl. Acad. Sci. USA 100:10446-10451.
15. Ingham, C. J., and P. A. Furneaux. 2000. Mutations in the ss subunit of the Bacillus subtilis RNA polymerase that confer both rifampicin resistance and hypersensitivity to NusG. Microbiology 146:3041-3049.
16. Krinos, C. M., M. J. Coyne, K. G. Weinacht, A. O. Tzianabos, D. L. Kasper, and L. E. Comstock. 2001. Extensive surface diversity of a commensal microorganism by multiple DNA inversions. Nature 414:555-558.
17. Kyrpides, N. C., C. R. Woese, and C. A. Ouzounis. 1996. KOW: a novel motif linking a bacterial transcription factor with ribosomal proteins. Trends Biochem. Sci. 21:425-426.
18. Lawrence, J. G., and J. R. Roth. 1996. Selfish operons: horizontal transfer may drive the evolution of gene clusters. Genetics 143:1843-1860.
19. Linn, T., and J. Greenblatt. 1992. The NusA and NusG proteins of Escherichia coli increase the in vitro readthrough frequency of a transcriptional attenuator preceding the gene for the beta subunit of RNA polymerase. J. Biol. Chem. 267:1449-1454.
20. Mason, S. W., and J. Greenblatt. 1991. Assembly of transcription elongation complexes containing the N protein of phage lambda and the Escherichia coli elongation factors NusA, NusB, NusG, and S10. Genes Dev. 5:1504-1512.
21. Nieto, J. M., M. J. Bailey, C. Hughes, and V. Koronakis. 1996. Suppression of transcription polarity in the Escherichia coli haemolysin operon by a short upstream element shared by polysaccharide and DNA transfer determinants. Mol. Microbiol. 19:705-713.
22. Pasman, Z., and P. H. von Hippel. 2000. Regulation of rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex. Biochemistry 39:5573-5585.
23. Smith, C. J., M. B. Rogers, and M. L. McKee. 1992. Heterologous gene expression in Bacteroides fragilis. Plasmid 27:141-154.
24. Squires, C. L., J. Greenblatt, J. Li, and C. Condon. 1993. Ribosomal RNA antitermination in vitro: requirement for Nus factors and one or more unidentified cellular components. Proc. Natl. Acad. Sci. USA 90:970-974.
25. Steiner, T., J. T. Kaiser, S. Marinkovic, R. Huber, and M. C. Wahl. 2002. Crystal structures of transcription factor NusG in light of its nucleic acid- and protein-binding activities. EMBO J. 21:4641-4653. (Pubitemid 34984351)
26. Thompson, J. S., and M. H. Malamy. 1990. Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus betalactamase II. J. Bacteriol. 172:2584-2593.
27. Zukowski, M. M., D. F. Gaffney, D. Speck, M. Kauffmann, A. Findeli, A. Wisecup, and J. P. Lecocq. 1983. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene. Proc. Natl. Acad. Sci. USA 80:1101-1105.