The use and value of in vitro technologies in metabolism studies

Alternatives to Laboratory Animals

Volumn 37, Issue SUPPL. 1, 2009, Pages 29-32

  Doehmer, Johannes ^a  

^a BioProof AG   (Germany)

Author keywords

Cytochrome P450; Heterologous expression; In vitro metabolism; V79 Chinese hamster cells

Indexed keywords

CYTOCHROME P450; ENZYME;

ANIMAL CELL; CELL LINE; CHO CELL; CONFERENCE PAPER; CONTROLLED STUDY; DRUG METABOLISM; DRUG SAFETY; FISH; GENE EXPRESSION; GENETIC ENGINEERING; HUMAN; HUMAN CELL; IN VITRO STUDY; LUNG FIBROBLAST; MEDICAL TECHNOLOGY; MOLECULAR CLONING; NONHUMAN; PREDICTION; PRIORITY JOURNAL;

ANIMALIA; CRICETULUS GRISEUS; RATTUS;

EID: 72249109867     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290903701s03     Document Type: Conference Paper

References (6)

1. Doehmer, J., Dogra, S., Friedberg, T., Monier, S., Adesnik, M., Glatt, H. & Oesch, F. (1988). Stable expression of rat cytochrome P450 IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1. Proceedings of the National Academy of Sciences of the USA 85, 5769-5773.
2. Doehmer, J. (2006). Predicting drug metabolism-dependent toxicity for humans with a genetically engineered cell battery. ATLA 34, 561-575.
3. Fuhr, U., Doehmer, J., Battula, N., Wölfel, C., Kudla, C., Keita, Y. & Staib, A.H. (1992). Biotransformation of caffeine and theophylline in mammalian cell lines genetically engineered for expression of single cytochrome P450 isoforms. Biochemical Pharmacology 43, 225-235.
4. Jensen, K.G., Loft, S., Doehmer, J. & Poulsen, H.E. (1993). Metabolism of phenacetin in V79 Chinese hamster cell cultures expressing rat liver cytochrome P450 1A2 compared to isolated rat hepatocytes. Biochemical Pharmacology 45, 1171-1173. (Pubitemid 23079998)
5. Stadler, J., Trockfeld, J., Schmalix, W.A., Brill, T., Siewert, J.R., Greim, H. & Doehmer, J. (1994). Inhibition of cytochromes P4501A by nitric oxide. Proceedings of the National Academy of Sciences of the USA 91, 3559-3563.
6. Krebsfaenger, N., Mürdter, T.E., Zanger, U.M., Eichelbaum, M.F. & Doehmer, J. (2003). V79 Chinese hamster cells genetically engineered for polymorphic cytochrome P450 2D6 and their predictive value for humans. ALTEX 20, 143-154.