Insecticidal activity of scorpion toxin (ButaIT) and snowdrop lectin (GNA) containing fusion proteins towards pest species of different orders

Pest Management Science

Volumn 66, Issue 1, 2010, Pages 74-83

  Fitches, Elaine C ^a   Bell, Howard A ^a   Powell, Michelle E ^a   Back, Emma ^b   Sargiotti, Chiara ^c   Weaver, Robert J ^a   Gatehouse, John A ^b  

^a FOOD AND ENVIRONMENT RESEARCH AGENCY   (United Kingdom)

^b DURHAM UNIVERSITY   (United Kingdom)

^c Isagro Ricerca srl   (Italy)

Author keywords

Arthropod toxins; Fusion protein; Novel insecticides; Recombinant protein

Indexed keywords

BUTHUS TAMULUS INSECT TOXIN; HYBRID PROTEIN; INSECTICIDE; MANNOSE BINDING LECTIN; PLANT LECTIN; SCORPION VENOM; SNOWDROP LECTIN;

BIOASSAY; INSECTICIDE; MORTALITY; PEST CONTROL; PROTEIN; SPIDER; TOXICITY;

ANIMAL; ARMYWORM; ARTICLE; BIOSYNTHESIS; DRUG EFFECT; HOUSE FLY; INJECTION; ISOLATION AND PURIFICATION; LARVA; TOXICITY TESTING; TRIBOLIUM;

ANIMALS; HOUSEFLIES; INJECTIONS; INSECTICIDES; LARVA; MANNOSE-BINDING LECTINS; PLANT LECTINS; RECOMBINANT FUSION PROTEINS; SCORPION VENOMS; SPODOPTERA; TOXICITY TESTS; TRIBOLIUM;

ARANEAE; ARTHROPODA; COLEOPTERA; DICTYOPTERA; DIPTERA; GALANTHUS; HEXAPODA; MUSCA DOMESTICA; SCORPIONES; SEGESTRIA FLORENTINA; SPODOPTERA LITTORALIS; TRIBOLIUM (BEETLE); TRIBOLIUM CASTANEUM;

EID: 72249106546     PISSN: 1526498X     EISSN: 15264998     Source Type: Journal    
DOI: 10.1002/ps.1833     Document Type: Article

References (26)

1. Casida JE and Quistad GB, Golden age of insecticide research: past, present, or future? Annu Rev Entomol 43:1-16 (1998).
2. Denholm I and Rowland MW, Tactics for managing pesticide resistance in arthropods - theory and practice. Annu Rev Entomol 37:91-112 (1992).
3. Desneux N, Decourtye A and Delpuech JM, The sublethal effects of pesticides on beneficial arthropods. Annu Rev Entomol 52:81-106 (2007).
4. van Emden HF and Peakhall DB, Beyond Silent Spring: Integrated Pes Management and Chemical Safety. Chapman and Hall, London, UK, pp. 1-322 (1996).
5. A thematic strategy on the sustainable use of pesticides, European Union, Communication from the Commission to the Council, The European Parliament, the European Economic and Social Committee and the Committee of the Regions, pp. 1-13 (2006).
6. Dhadialla TS, Carlson GR and Le DP, New insecticides with ecdysteroidal and juvenile hormone activity. Annu Rev Entomol 43:545-569 (1998).
7. Isman MB, Botanical insecticides, deterrents, and repellents inmodern agriculture and an increasingly regulated world. Annu Rev Entomol 51:45-66 (2006).
8. Beckage NE and Gelman DB, Wasp parasitoid disruption of host development: implications for new biologically based strategies for insect control. Annu Rev Entomol 49:299-330 (2004).
9. Fitches E, Gatehouse AMR and Gatehouse JA, Effects of snowdrop lectin (GNA) delivered via artificial diet and transgenic plants on the development of tomato moth (Lacanobia oleracea) larvae in laboratory and glasshouse trials. J Insect Physiol 43:727-739 (1997).
10. Fitches E, Woodhouse SD, Edwards JP and Gatehouse JA, In vitro and in vivo binding of snowdrop (Galanthus nivalis agglutinin; GNA) and jackbean (Canavalia ensiformis; Con A) lectins within tomato moth(Lacanobiaoleracea) larvae;mechanismsof insecticidal action. J Insect Physiol 47:777-787 (2001).
11. Fitches E, Audsley N, Gatehouse JA and Edwards JP, Fusion proteins containing neuropeptides as novel insect control agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion. Insect BiochemMolec Biol 32:1653-1661 (2002).
12. Fitches E, Edwards MG,Mee C, Grishin E, Gatehouse AMR, Edwards JP, et al., Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venomtoxin to insect haemolymph following oral ingestion. J Insect Physiol 50:61-71 (2004).
13. Down RE, Fitches EC, Wiles DP, Corti P, Bell HA, Gatehouse JA, et al., Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) and the rice brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). PestManag Sci 62:77-85 (2006).
14. Wudayagiri R, Inceoglu B, Herrmann R, Derbel M, Choudary PV and Hammock BD, Isolation and characterization of a novel lepidopteran-selective toxin from the venom of South Indian red scorpion, Mesobuthus tamulus. BMC Biochem 2:11-16 (2001).
15. Trung NP, Fitches E and Gatehouse JA, A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects. BMC Biotechnol 6:18 (2006).
16. Sambrook J and Russell DW, Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New York, NY (2001).
17. Schägger H, Tricine-SDS-PAGE. Nat Protoc 1:16-22 (2006).
18. Fitches E and Gatehouse JA, A comparison of the short and long term effects of insecticidal lectins on the activities of soluble and brush border enzymes of tomato moth larvae (Lacanobia oleracea). J Insect Physiol 44:1213-1224 (1998).
19. Raemaekers RJM, de Muro L, Gatehouse JA and Fordham-Skelton AP, Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris - correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. Eur J Biochem 265:394-403 (1999).
20. Poitout S and Bues R, Elevage de plusiers espéces de L ́epidoptéres Noctuidae sur milieu artificiel simplifi ́ e. Ann Zoo Ecol Anim 2:79-91 (1970).
21. Bryning GP, Chambers J and Wakefield ME, Identification of a sex pheromone from male yellow mealwormbeetles, Tenebrio molitor. J ChemEcol 31:2721-2730 (2005).
22. Short JE and Edwards JP, Reproductive and developmental biology of the oriental cockroach Blatta orientalis (Dictyoptera). Med Vet Entomol 5:385-394 (1991).
23. Barson G, Renn Nand Bywater AF, Laboratory evaluation of six species of entomopathogenic fungi for the control of the house fly (Musca domestica L.), a pest of intensive animal units. J Invertebr Pathol 64:107-113 (1994).
24. Abbott WS, A method for computing the effectiveness of an insecticide. J Econ Entomol 18:265-267 (1925).
25. Lipkin A, Kozlov S, Nosyreva E, Blake A, Windass JD and Grishin E, Novel insecticidal toxins from the venom of the spider Segestria florentina. Toxicon 40:125-130 (2002).
26. Down RE, Gatehouse AMR, Hamilton WDO and Gatehouse JA, Snowdrop lectin inhibits development and decreases fecundity of the glasshouse potato aphid (Aulacorthum solani) when administered in vitro and via transgenic plants both in laboratory and glasshouse trials. J Insect Physiol 42:1035-1045 (1996).