Long fluorescence lifetime molecular probes based on near infrared pyrrolopyrrole cyanine fluorophores for in vivo imaging

Biophysical Journal

Volumn 97, Issue 9, 2009, Pages

  Berezin, Mikhail Y ^a   Akers, Walter J ^a   Guo, Kevin ^a   Fischer, Georg M ^b   Daltrozzo, Ewald ^b   Zumbusch, Andreas ^b   Achilefu, Samuel ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PYRROLOPYRROLE CYANINE DYE; UNCLASSIFIED DRUG;

ARTICLE; AUTOFLUORESCENCE; FLUORESCENCE; INFRARED RADIATION; MOLECULAR IMAGING; MOLECULAR PROBE; NEAR INFRARED SPECTROSCOPY; NONHUMAN; SPECTRAL SENSITIVITY;

MUS;

EID: 72249091569     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.08.022     Document Type: Article

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28. In vivo imaging. After injections, the animals were positioned prone on the heated imaging platform and a 2D scanning region of interest was selected by top-view charge-coupled device camera to include the area from the neck to the pelvis. The 780-nm pulsed diode laser was set to 0.4 μW for excitation scans and adjusted for optimal signal strength, in the range 5-100 μW for fluorescence detection. Regions of interest were raster-scanned in 3-mm increments for contrast agent imaging with 0.3 s integration time per pixel. For more details see Akers et al. (18).