Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology

Journal of Bioscience and Bioengineering

Volumn 109, Issue 1, 2010, Pages 15-19

  Ohiro, Yoshiyuki ^a   Ueda, Hiroshi ^b   Shibata, Norio ^a   Nagamune, Teruyuki ^b  

^a EIKEN CHEMICAL CO LTD   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Antibody; Extracellular signal regulated kinase; Fluorescence resonance energy transfer; Green fluorescent protein; Homogeneous immunoassay; Phosphorylation

Indexed keywords

ANTIGEN PEPTIDE; COMPETITIVE IMMUNOASSAY; ENHANCED FLUORESCENCE; EXTRACELLULAR SIGNAL-REGULATED KINASE; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT SPECTRA; GREEN FLUORESCENT PROTEIN; HOMOGENEOUS IMMUNOASSAY; IN-VITRO ASSAYS; IN-VIVO; LEUCINE ZIPPERS; MOLECULAR PROBES; PHOSPHORYLATED PROTEINS; PHOSPHORYLATION SITES; PROTEIN PHOSPHORYLATION; SPECIFIC INTERACTION;

AMINES; AMINO ACIDS; ANTIBODIES; ENERGY TRANSFER; FLUORESCENCE; IMMUNOLOGY; PHOSPHORYLATION; PROBES; RESONANCE; TRANSCRIPTION FACTORS;

ANTIGENS;

LEUCINE ZIPPER PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE;

ARTICLE; CONJUGATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMMUNOASSAY; MOLECULAR PROBE; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION;

ANTIGENS; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMMUNOASSAY; KINETICS; PHOSPHORYLATION; PROTEIN BINDING; SENSITIVITY AND SPECIFICITY;

EID: 72249087526     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.07.004     Document Type: Article

References (20)

1. Inagaki M., Nishi Y., Nishizawa K., Matsuyama M., and Sato C. Site-specific phosphorylation induces disassembly of vimentin filaments in vitro. Nature 328 (1987) 649-652
2. Lange-Carter C.A., and Johnson G.L. Ras-dependent growth factor regulation of MEK kinase in PC12 cells. Science 265 (1994) 1458-1461
3. Ullrich S.J., Anderson C.W., Mercer W.E., and Appella E. The p53 tumor suppressor protein, a modulator of cell proliferation. J. Biol. Chem. 267 (1992) 15259-15262
4. Kameshita I., and Fujisawa H. A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 183 (1989) 139-143
5. Whitmarsh A.J., and Davis R.J. Analyzing JNK and p38 mitogen-activated protein kinase activity. Methods Enzymol. 332 (2001) 319-336
6. Bardwell L., and Shah K. Analysis of mitogen-activated protein kinase activation and interactions with regulators and substrates. Methods 40 (2006) 213-223
7. Goshe M.B. Characterizing phosphoproteins and phosphoproteomes using mass spectrometry. Brief Funct. Genomic Proteomic 4 (2006) 363-376
8. Ishida A., Kameshita I., Sueyoshi N., Taniguchi T., and Shigeri Y. Recent advances in technologies for analyzing protein kinases. J. Pharmacol. Sci. 103 (2007) 5-11
9. Yau L., and Zahradka P. Immunodetection of activated mitogen-activated protein kinase in vascular tissues. Mol. Cell. Biochem. 172 (1997) 59-66
10. Mandell J.W. Phosphorylation state-specific antibodies: applications in investigative and diagnostic pathology. Am. J. Pathol. 163 (2003) 1687-1698
11. Ohiro Y., Arai R., Ueda H., and Nagamune T. A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction. Anal. Chem. 74 (2002) 5786-5792
12. Ohiro Y., Ueda H., Shibata N., and Nagamune T. Enhanced fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab′-based immunoconjugates. Anal. Biochem. 360 (2007) 266-272
13. Printen J.A., and Sprague Jr. G.F. Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics 138 (1994) 609-619
14. Fukuda M., Gotoh Y., and Nishida E. Interaction of MAP kinase with MAP kinase kinase: its possible role in the control of nucleocytoplasmic transport of MAP kinase. EMBO J. 16 (1997) 1901-1908
15. Pearson G., Robinson F., Beers Gibson T., Xu B.E., Karandikar M., Berman K., and Cobb M. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22 (2001) 153-183
16. Torii S., Nakayama K., Yamamoto T., and Nishida E. Regulatory mechanisms and function of ERK MAP kinases. J. Biochem. 136 (2004) 557-561
17. Meyerhoff M.E., and Rechnitz G.A. Electrode-based enzyme immunoassays using urease conjugates. Methods Enzymol. 70 (1980) 439-454
18. Shono F., Yokota K., Horie K., Yamamoto S., Yamashita K., Watanabe K., and Miyazaki H. A heterologous enzyme immunoassay of prostaglandin E2 using a stable enzyme-labeled hapten mimic. Anal. Biochem. 168 (1988) 284-291
19. Huang C.Y., and Ferrell Jr. J.E. Ultrasensitivity in the mitogen-activated protein kinase cascade. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 10078-10083
20. Fujioka A., Terai K., Itoh RE., Aoki K., Nakamura T., Kuroda S., Nishida E., and Matsuda M. Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. J. Biol. Chem. 281 (2006) 8917-8926