Determination of molecular mass values of chondroitin sulfates by fluorophore-assisted carbohydrate electrophoresis (FACE)

Journal of Pharmaceutical and Biomedical Analysis

Volumn 51, Issue 4, 2010, Pages 969-972

  Buzzega, Dania ^a   Maccari, Francesca ^a   Volpi, Nicola ^a  

^a UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

Chondroitin sulfate; Electrophoresis; FACE; Glycosaminoglycans; Molecular mass

Indexed keywords

CHONDROITIN SULFATE;

ARTICLE; CALIBRATION; DENSITOMETRY; DISPERSION; DRUG DETERMINATION; DRUG PURIFICATION; ELECTROPHORESIS; FLUOROPHORE ASSISTED CARBOHYDRATE ELECTROPHORESIS; HIGH PERFORMANCE SIZE EXCLUSION LIQUID CHROMATOGRAPHY; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; MACROMOLECULE; MOLECULAR WEIGHT; PRIORITY JOURNAL; QUALITY CONTROL; STANDARDIZATION; STATISTICAL ANALYSIS;

ANIMALS; CALIBRATION; CATTLE; CHICKENS; CHONDROITIN SULFATES; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS; FLUORESCENT DYES; MOLECULAR WEIGHT; NAPHTHALENES; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SWINE;

EID: 72149119707     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2009.10.015     Document Type: Article

References (11)

1. In: Volpi N. (Ed). Chondroitin Sulfate: Structure, Role and Pharmacological Activity (2006), Academic Press, Amsterdam/Boston/Heidelberg/London/New York/Oxford/Paris/San Diego/San Francisco/Singapore/Sydney/Tokyo
2. Volpi N. Analytical aspects of pharmaceutical grade chondroitin sulfates. J. Pharm. Sci. 96 (2007) 3168-3180
3. Volpi N. Quality of different chondroitin sulfate preparations in relation to their therapeutic activity. J. Pharm. Pharmacol. 61 (2009) 1271-1280
4. Uebelhart D., Knols R., de Bruin E.D., and Verbruggen G. Treatment of knee osteoarthritis with oral chondroitin sulfate. Adv. Pharmacol. 53 (2006) 475-488
5. Kahan A., Uebelhart D., De Vathaire F., Delmas P.D., and Reginster J.Y. Long-term effects of chondroitins 4 and 6 sulfate on knee osteoarthritis: the study on osteoarthritis progression prevention, a two-year, randomized, double-blind, placebo-controlled trial. Arthritis Rheum. 60 (2009) 524-533
6. Buzzega D., Maccari F., and Volpi N. Fluorophore-assisted carbohydrate electrophoresis for the determination of molecular mass of heparins and low-molecular-weight (LMW) heparins. Electrophoresis 29 (2008) 4192-4202
7. In: Ofosu F.A., Danishefsky I., and Hirsh J. (Eds). Heparin and Related Polysaccharides. Structure and Activities vol. 556 (1989), N.Y. Acad. Sci., New York
8. Desai U.R., and Linhardt R.J. Molecular weight of heparin using 13C nuclear magnetic resonance spectroscopy. J. Pharm. Sci. 84 (1995) 212-215
9. Volpi N., Fregni R., and Venturelli T. Activity of chondroitin ABC lyase on dermatan sulfate partially degraded by cupric-ion-mediated free-radical treatment. J. Chromatogr. Biomed. Appl. 669 (1995) 197-205
10. Volpi N. Disaccharide analysis and molecular mass determination to microgram level of single sulfated glycosaminoglycan species in mixtures following agarose-gel electrophoresis. Anal. Biochem. 273 (1999) 229-239
11. Oonuki Y., Yoshida Y., Uchiyama Y., and Asari A. Application of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans. Anal. Biochem. 343 (2005) 212-222