Isolation of sea squirt (Ciona) gametes, fertilization, dechorionation, and development

Cold Spring Harbor Protocols

Volumn 4, Issue 12, 2009, Pages

  Christiaen, Lionel ^a,b   Wagner, Eileen ^a   Shi, Weiyang ^a   Levine, Michael ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NEW YORK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL ISOLATION; CELL MATURATION; CHORION; CIONA INTESTINALIS; CLINICAL PROTOCOL; CULTURE MEDIUM; ELECTROPORATION; EMBRYO; EMBRYO CULTURE; FERTILIZATION; GAMETE; IN SITU HYBRIDIZATION; MICROINJECTION; NONHUMAN; SPECIES CULTIVATION; SPERM; TRANSGENE;

CIONA;

EID: 72149087973     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5344     Document Type: Article

References (3)

1. Christiaen L, Wagner E, Shi W, Levine M. 2009a. The sea squirt Ciona intestinalis. Cold Spring Harb Protoc (this issue), doi: 10.1101/ pdb.emo138.
2. Christiaen L, Wagner E, Shi W, Levine M. 2009b. Electroporation of transgenic DNAs in the sea squirt Ciona. Cold Spring Harb Protoc (this issue), doi: 10.1101/pdb.prot5345.
3. Christiaen L, Wagner E, Shi W, Levine M. 2009c. Microinjection of morpholino oligos and RNAs in sea squirt (Ciona) embryos. Cold Spring Harb Protoc (this issue), doi: 10.1101/pdb.prot5347.