Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 877, Issue 31, 2009, Pages 4015-4021

  Wu, Yue Jin ^a   Fan, Chia Ying ^a   Li, Yaw Kuen ^a  

^a NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

Author keywords

Auto cleavage; Chitin column; Peptide linker; Protein purification

Indexed keywords

AFFINITY BINDING; CHITIN-BINDING; E. COLI; EXPRESSION VECTORS; FUNCTIONALIZED; FUSION PROTEINS; GEL-FILTRATION; GLUTATHIONES; NICKEL COLUMN; NUCLEOTIDE SEQUENCES; PEPTIDE LINKERS; PH-DEPENDENT; PROTEASE DIGESTION; PROTEIN PURIFICATION; TARGET GENES; TARGET PROTEINS;

CHITIN; CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; GELS; HYDROPHOBIC CHROMATOGRAPHY; ION EXCHANGE; PEPTIDES; PURIFICATION;

COLUMN CHROMATOGRAPHY;

BINDING PROTEIN; CHITIN BINDING PROTEIN; HYBRID PROTEIN; PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; GENE INSERTION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PURIFICATION; RESTRICTION SITE;

BACTERIAL PROTEINS; CHITIN; CLONING, MOLECULAR; HYDROGEN-ION CONCENTRATION; PEPTIDES; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 72049105108     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2009.10.009     Document Type: Article

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