A cross comparison of QPCR to agar-based or defined substrate test methods for the determination of Escherichia coli and enterococci in municipal water quality monitoring programs

Water Research

Volumn 43, Issue 19, 2009, Pages 4967-4979

  Lavender, Jennifer S ^a   Kinzelman, Julie L ^a  

^a City of Racine Health Department   (United States)

Author keywords

Beaches; E. coli; Enterococci; QPCR; Stormwater; Wastewater

Indexed keywords

ALGAE; BEACHES; POLYMERASE CHAIN REACTION; POLYSACCHARIDES; STORMS; SURFACE WATERS; TESTING; WASTEWATER TREATMENT; WATER QUALITY;

E. COLI; ENTEROCOCCUS; MOLECULAR METHODS; MONITORING PROGRAMMES; MUNICIPAL WATER; QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION; STORMWATERS; TEST METHOD; WATER QUALITY MONITORING; WATER SAMPLES;

ESCHERICHIA COLI;

FRESH WATER; RIVER WATER; STORM WATER; SURFACE WATER;

BEACH; BIOINDICATOR; BIOMONITORING; COLIFORM BACTERIUM; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; STORMWATER; SURFACE WATER; TESTING METHOD; WASTEWATER; WATER QUALITY;

AGAR MEDIUM; ARTICLE; BACTERIAL COUNT; BACTERIAL VIABILITY; BACTERIUM DETECTION; CONTROLLED STUDY; ENTEROCOCCUS; ENVIRONMENTAL MONITORING; ESCHERICHIA COLI; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; UNITED STATES; WASTE WATER MANAGEMENT; WATER ANALYSIS; WATER QUALITY; WATER SAMPLING;

ESCHERICHIA COLI;

EID: 71949129402     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2009.08.010     Document Type: Article

References (37)

1. Abbott, K., 2008. Guiding remediation of the Root River, Racine, WI, USA, through Correlation of Stream Bank Condition, River Morphology and Infrastructure Surveys to Chemical and Microbial Source Tracking. MS thesis, University of Surrey, Guildford, Surrey, UK.
2. Anderson S., Turner S., and Lewis G. Enterococci in the New Zealand environment: implications for water quality monitoring. Water Science and Technology 35 (1997) 325-331
3. Blackwood A., and Noble R. Comparison of TaqMan and Scorpion Multiplex Assays for Use as Indicators of Fecal Contamination in Environmental Waters. ASM National Meeting, June 2005 (2005), ASM Press, Atlanta, GA
4. Blackwood A., Yu S., Gregory J., and Noble R. Rapid QPCR assays for Escherichia coli and Enterococcus in recreational waters: equivalent to existing methods?. ASM National Meeting, May 2006, Orlando, FL (2006), ASM Press, Washington DC
5. Brinkman N., Haugland R., Wymer L., Byappanahalli M., Whitman R., and Vesper S. Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water. Applied and Environmental Microbiology 69 3 (2003) 1775-1782
6. Cabelli V., Dufour A., McCabe L., and Levin M. Swimming associated gastroenteritis and water quality. American Journal of Epidemiology 115 (1982) 606-616
7. Colford J., Wade T., Schiff K., Wright C., Griffith J., Sandhu S., Burns S., Sobsey M., Lovelace G., and Weisberg S. Water quality indicators and the risk of illness at beaches with nonpoint sources of fecal contamination. Epidemiology 18 1 (2007) 27-35
8. Dupray E., Caprais M., Derrien A., and Fach P. Salmonella DNA persistence in natural seawaters using PCR analysis. Journal of Applied Microbiology 82 4 (1997) 507-510
9. Dufour A. Health Effects Criteria for Fresh Recreational Waters. United States Environmental Protection Agency (1984) EPA-600/1-84-004
10. Elkind M., and Han A. DNA single-strand lesions due to 'sunlight' and UV light: a comparison of their induction in Chinese hamster and human cells, and their fate in Chinese hamster cells. Photochemistry and Photobiology 27 6 (1978) 717-724
11. Frahm E., and Obst U. Application of the fluorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples. Journal of Microbiological Methods 52 1 (2003) 123-131
12. Fujioka R., and Narikawa O. Effect of sunlight on enumeration of indicator bacteria under field conditions. Applied and Environmental Microbiology 44 2 (1982) 395-401
13. Genthner F., James J., Yates D., and Friedman S. Use of composite data sets for source-tracking enterococci in the water column and shoreline interstitial waters on Pensacola Beach, Florida. Marine Pollution Bulletin 50 (2005) 724-732
14. Gilbride K., Lee D., and Beaudette L. Molecular techniques in wastewater: understanding microbial communities, detecting pathogens, and real-time process control. Journal of Microbiological Methods 66 (2006) 1-20
15. Griffith, J., Weisberg, S., 2006. Evaluation of New, Rapid Microbiological Methods For Measuring Recreational Water Quality. 2006 Southern California Coastal Water Research Project, Technical Report #485.
16. Haile, R., Alamillo, J., Barrett, K., Cressey, R., Dermond, J., Ervin, C., Glasser, A., Harawa, N., Harmon, P., Harper, J., McGee, C., Millikan, R., Nides, M., Witte, J., 1996. An Epidemiological Study of Possible Health Effects of Swimming in Santa Monica Bay. Report of Santa Monica Bay restoration project - Final Report, Regional Water Quality Control Board, Los Angeles Region, Monterey Park, CA.
17. Haugland R., Vesper S., and Wymer L. Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqMan™ fluorogenic probe system. Molecular and Cellular Probes 13 (1999) 329-340
18. Haugland R., Siefring S., Wymer L., Brenner K., and Dufour A. Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis. Water Research 39 4 (2005) 559-568
19. He J., and Jiang S. Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR. Applied and Environmental Microbiology 71 5 (2005) 2250-2255
20. Heid C., Stevens J., Livak K., and Williams M. Real time quantitative PCR. Genome Research 6 10 (1996) 986-994
21. Holland P., Abramson R., Watson R., and Gelfand D. Detection of specific polymerase chain reaction product by utilizing the 5′ → 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences 88 16 (1991) 7276-7280
22. Khan I., Gannon V., Kent R., Koning W., Lapen D., Miller J., Neumann N., Phillips R., Robertson W., Topp E., van Bochove E., and Edge T. Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds. Journal of Microbiological Methods 69 3 (2007) 480-488
23. Kinzelman, J., 2005. Investigating Bathing Water Quality Failures and Initiating Remediation for the Protection of Public Health. PhD dissertation, University of Surrey, Guildford, Surrey, UK.
24. Kinzelman J., Dufour A., Wymer L., Rees G., Pond K., and Bagley R. Comparison of multiple point and composite sampling for monitoring bathing water quality. Lake and Reservoir Management 22 2 (2006) 95-102
25. Kinzelman, J. Preparing a Local Public Health Laboratory for QPCR. In: 7th Annual Great Lakes Beach Association Meeting, Traverse City, Michigan, October 3-5, 2007.
26. Lavender, J., 2008. Determination of Pollutant Loading and Ambient Escherichia coli and Enterococci DNA Levels in Fresh Water Environments by Quantitative Real-Time PCR. MS thesis, University of Wisconsin Parkside, Kenosha, WI, USA.
27. Meador J., Baldwin A., Catala P., Jeffrey W., Joux F., Moss J., Dean Pakulski J., Stevens R., and Mitchell D. Sunlight-induced DNA damage in marine micro-organisms collected along a latitudinal gradient from 70°N to 68°S. Photochemistry and Photobiology 85 1 (2009) 412-420
28. Murray C., Sohngen B., and Pendleton L. Valuing water quality advisories and beach amenities in the Great Lakes. Water Resources Research 37 10 (2001) 2583-2590
29. Noble R., Leecaster M., McGee C., Weisberg S., and Ritter K. Comparison of bacterial indicator analysis methods in stormwater-affected coastal waters. Water Research 38 5 (2004) 1183-1188
30. Noble R., Griffith J., Blackwood A.D., Fuhrman J., Gregory J., Hernandez X., Liang X., Bera A., and Schiff K. Multitiered approach using quantitative PCR to track sources of fecal pollution affecting Santa Monica Bay, California. Applied and Environmental Microbiology 72 2 (2006) 1604-1612
31. Signoretto C., Burlacchini G., del Mar Lle M., Pruzzo C., Zampini M., Pane L., Franzini G., and Canepari P. Adhesion of Enterococcus faecalis in the nonculturable state to plankton is the main mechanism responsible for persistence of this bacterium in both lake and seawater. Applied and Environmental Microbiology 70 11 (2004) 6892-6896
32. US EPA., 1972. Federal Water Pollution Control Amendments of 1972. In: 92nd United States Congress, Public Law 92-500.
33. US EPA. Ambient Water Quality Criteria For Bacteria. Office of Water Regulation and Standards, Criteria and Standards Division (1986), United States Environmental Protection Agency, Washington, D.C EPA 440/5-84-002
34. US EPA., 2000. Beaches Environmental Assessment and Coastal Health Act of 2000. In: 106th United States Congress. Public Law 106-284.
35. US EPA. Method 1600: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-ß-D-Glucoside Agar (mEI) (2002), United States Environmental Protection Agency, Washington, DC EPA 821-R-02-022
36. US EPA. Water quality standards for coastal and great lakes recreation waters; final rule 40 CFR part 131. Federal Register 69 220 (2004) 67218-67243
37. Wade T., Calderon R., Sams E., Beach M., Brenner K., Williams A., and Dufour A. Rapidly measured indicators of recreational water quality are predictive of swimming-associated gastrointestinal illness. Environmental Health Perspectives 114 1 (2006) 24-28