Cytokine mRNA expression profiles in peripheral blood mononuclear cells from piglets experimentally co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2

Veterinary Microbiology

Volumn 140, Issue 1-2, 2010, Pages 155-160

  Shi, Kai Chuang ^a,b   Guo, Xin ^a   Ge, Xin Na ^a   Liu, Qi ^b   Yang, Han Chun ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b Guangxi Center for Animal Disease Control and Prevention   (China)

Author keywords

Co infection; Cytokine; Immunomodulation; Immunosuppression; Porcine circovirus type 2 (PCV2); Porcine reproductive and respiratory syndrome virus (PRRSV)

Indexed keywords

CONCANAVALIN A; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 12P40; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 6; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTERIVIRUS; ARTICLE; CELLULAR IMMUNITY; CIRCOVIRIDAE; CONTROLLED STUDY; HOST; HUMORAL IMMUNITY; IMMUNE RESPONSE; MIXED INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PERIPHERAL BLOOD MONONUCLEAR CELL; PIGLET; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEROLOGY; STIMULATION; TH1 CELL; TH2 CELL; UPREGULATION; VIROLOGY;

ADJUVANTS, IMMUNOLOGIC; ANIMALS; ANTIBODIES, VIRAL; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; CONCANAVALIN A; CYTOKINES; LEUKOCYTES, MONONUCLEAR; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; RNA, MESSENGER; SWINE; SWINE DISEASES; UP-REGULATION;

PORCINE CIRCOVIRUS 2; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE;

EID: 71849119440     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2009.07.021     Document Type: Article

References (29)

1. Allan G.M., and Ellis J.A. Porcine circoviruses: a review. J. Vet. Diagn. Invest. 12 (2000) 3-4
2. Asai T., Mori M., Okada M., Uruno K., Yazawa S., and Shibata I. Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopathol. 70 (1999) 143-148
3. Bautista E.M., and Molitor T.W. IFN-gamma inhibits porcine reproductive and respiratory syndrome virus replication in macrophages. Arch. Virol. 144 (1999) 1191-1200
4. Charerntantanakul W., Platt R., and Roth J.A. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 19 (2006) 646-661
5. Choi C., Cho W.S., Kim B., and Chae C. Expression of interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV). J. Comp. Pathol. 127 (2002) 106-113
6. Chung H.K., and Chae C. Expression of interleukin-10 and interleukin-12 in piglets experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV). J. Comp. Pathol. 129 (2003) 205-212
7. Darwich L., Pié S., Rovira A., Segalés J., Domingo M., Oswald I.P., and Mateu E. Cytokine mRNA expression profiles in lymphoid tissues of pigs naturally affected by postweaning multisystemic wasting syndrome. J. Gen. Virol. 84 (2003) 2117-2125
8. Darwich L., Balasch M., Plana-Durán J., Segalés J., Domingo M., and Mateu E. Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J. Gen. Virol. 84 (2003) 3453-3457
9. Darwich L., Segalés J., Resendes A., Balasch M., Plana-Durán J., and Mateu E. Transient correlation between viremia levels and IL-10 expression in pigs subclinically infected with porcine circovirus type 2 (PCV2). Res. Vet. Sci. 84 (2008) 194-198
10. Díaz I., Darwich L., Pappaterra G., Pujols J., and Mateu E. Immune responses of pigs after experimental infection with a European strain of porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 86 (2005) 1943-1951
11. Feng W.H., Tompkins M.B., Xu J.S., Zhang H.X., and McCaw M.B. Analysis of constitutive cytokine expression by pigs infected in-utero with porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopathol. 94 (2003) 35-45
12. Grau-Roma L., and Segalés J. Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza virus and Aujeszky's disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain. Vet. Microbiol. 119 (2007) 144-151
13. Johnsen C.K., Bøtner A., Kamstrup S., Lind P., and Nielsen J. Cytokine mRNA profiles in bronchoalveolar cells of piglets experimentally infected in utero with porcine reproductive and respiratory syndrome virus: association of sustained expression of IFN-γ and IL-10 after viral clearance. Viral Immunol. 15 (2002) 549-556
14. Kekarainen T., Montoya M., Mateu E., and Segalés J. Porcine circovirus type 2-induced interleukin-10 modulates recall antigen responses. J. Gen. Virol. 89 (2008) 760-765
15. Kim J., Ha Y., and Chae C. Potentiation of porcine circovirus 2-induced postweaning multisystemic wasting syndrome by porcine parvovirus is associated with excessive production of tumor necrosis factor-alpha. Vet. Pathol. 43 (2006) 718-725
16. López Fuertes L., Doménech N., Alvarez B., Ezquerra A., Domínguez J., Castro J.M., and Alonso F. Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection. Virus Res. 64 (1999) 33-42
17. Mateu E., and Díaz I. The challenge of PRRS immunology. Vet. J. 177 (2008) 345-351
18. Meerts P., Misinzo G., and Nauwynck H.J. Enhancement of porcine circovirus 2 replication in porcine cell lines by IFN-γ before and after treatment and by IFN-α after treatment. J. Interferon Cytokine Res. 25 (2005) 684-693
19. Murtaugh M.P., Xiao Z., and Zuckermann F. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol. 15 (2002) 533-547
20. Rossow K.D. Porcine reproductive and respiratory syndrome. Vet. Pathol. 35 (1998) 1-20
21. Segalés J., Domingo M., Chianini F., Majó N., Domínguez J., Darwich L., and Mateu E. Immunosuppression in postweaning multisystemic wasting syndrome affected pigs. Vet. Microbiol. 98 (2004) 151-158
22. Shi K., Li H., Guo X., Ge X., Jia H., Zheng S., and Yang H. Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet. Microbiol. 129 (2008) 367-377
23. Sipos W., Duvigneau J.C., Willheim M., Schilcher F., Hartl R.T., Hofbauer G., Exel B., Pietschmann P., and Schmoll F. Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection. Vet. Immunol. Immunopathol. 99 (2004) 63-71
24. Spagnuolo-Weaver M., Fuerst R., Campbell S.T., Meehan B.M., McNeilly F., Adair B., and Allan G. A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines. J. Immunol. Methods 230 (1999) 19-27
25. Stevenson L.S., McCullough K., Vincent I., Gilpin D.F., Summerfield A., Nielsen J., McNeilly F., Adair B.M., and Allan G.M. Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets. Viral Immunol. 19 (2006) 189-195
26. Suradhat S., and Thanawongnuwech R. Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus. J. Gen. Virol. 84 (2003) 2755-2760
27. Thanawongnuwech R., Thacker B., Halbur P., and Thacker E.L. Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Clin. Diagn. Lab. Immunol. 11 (2004) 901-908
28. Van Gucht S., Van Reeth K., and Pensaert M. Interaction between porcine reproductive-respiratory syndrome virus and bacterial endotoxin in the lungs of pigs: potentiation of cytokine production and respiratory disease. J. Clin. Microbiol. 41 (2003) 960-966
29. Wesley R.D., Lager K.M., and Kehrli Jr. M.E. Infection with porcine reproductive and respiratory syndrome virus stimulates an early gamma interferon response in the serum of pigs. Can. J. Vet. Res. 70 (2006) 176-182