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In this study the authors used a biochemical approach to provided mechanistically inside how the nontranscriptional effect of Sox9 on beta-catenin levels, which had been reported previously by Akiyama et al. [59], is accomplished.
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This in vivo study in the mouse has provided evidence that the interactions of Twist1 and Runx2 are not only involved in regulating osteoblastogenesis, but also important for chondrocyte hypertrophy. The results suggest that Runx2 in addition to promoting hypertrophy through its expression in chondrocytes, negatively regulates hypertrophy by acting in the perichondrium thereby providing an additional level of control in order to coordinate chondrocyte maturation and osteoblastogenesis.
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