Diagnostic value of an enzyme-linked immunosorbent assay using the recombinant CT694 species-specific protein of Chlamydia trachomatis

Journal of Applied Microbiology

Volumn 107, Issue 6, 2009, Pages 1875-1882

  Frikha Gargouri, O ^a   Gdoura, R ^a   Znazen, A ^a   Gargouri, J ^b   Rebai, A ^c   Hammami, A ^a  

^a HABIB BOURGUIBA HOSPITAL   (Tunisia)

^b Department of Blood Bank ^*  (Tunisia)

^c CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

Author keywords

Bioinformatic tools; Chlamydia trachomatis; CT694 ELISA; CT694 hypothetical protein; Expression vector; Microimmunofluorescence

Indexed keywords

CHEMICAL DETECTION; DIAGNOSIS;

BIOINFORMATIC TOOLS; C.TRACHOMATIS; CHLAMYDIA TRACHOMATIS; CT694 ELISA; CT694 HYPOTHETICAL PROTEIN; ELISA TEST; EXPRESSION VECTORS; HYPOTHETICAL PROTEIN; MICROIMMUNOFLUORESCENCE; PERFORMANCE;

RECOMBINANT PROTEINS;

BACTERIAL DNA; BACTERIAL PROTEIN; IMMUNOGLOBULIN G ANTIBODY; RECOMBINANT PROTEIN;

ANTIBODY; ANTIGEN; BACTERIAL DISEASE; BIOINFORMATICS; BLOOD; CHILD HEALTH; ENZYME ACTIVITY; IMMUNOASSAY; PROTEIN; RECOMBINATION;

ADULT; AMINO ACID SEQUENCE; ANTIBODY DETECTION; ARTICLE; BIOINFORMATICS; BLOOD DONOR; CHILD; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; HIGH RISK POPULATION; HUMAN; IMMUNOFLUORESCENCE; INTERMETHOD COMPARISON; MICROIMMUNOFLUORESCENCE; NONHUMAN; NORMAL HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROSTITUTION; PROTEIN EXPRESSION; SERODIAGNOSIS; SERUM; SEXUALLY TRANSMITTED DISEASE;

CHLAMYDIA TRACHOMATIS; CHLAMYDOPHILA PNEUMONIAE;

EID: 71749121621     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04365.x     Document Type: Article

References (24)

1. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248 254.
2. Frikha-Gargouri, O., Znazen, A., Gdoura, R., Gargouri, B., Arab, N.B., Ben Jemaa, M. Hammami, A. (2008a) Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections. Pathol Biol 56, 143 147.
3. Frikha-Gargouri, O., Gdoura, R., Znazen, A., Gargouri, B., Gargouri, J., Rebai, A. Hammami, A. (2008b) Evaluation of an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of Chlamydia trachomatis infections. BMC Microbiol 8, 217.
4. Gijsen, A.P., Land, J.A., Goossens, V.J., Leffers, P., Bruggeman, C.A. Evers, J.L. (2001) Chlamydia pneumoniae and screening for tubal factor subfertility. Hum Reprod 16, 487 491.
5. Greiner, M., Sohr, D. Gobel, P. (1995) A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. J Immunol Methods 185, 123 132.
6. Griffiths, E., Ventresca, M.S. Gupta, R.S. (2006) BLAST screening of chlamydial genomes to identify signature proteins that are unique for the Chlamydiales, Chlamydiaceae, Chlamydophila and Chlamydia groups of species. BMC Genomics 7, 14.
7. Jackson, L.A., Cherry, J.D., Wang, S.P. Grayston, J.T. (2000) Frequency of serological evidence of Bordetella infections and mixed infections with other respiratory pathogens in university students with cough illnesses. Clin Infect Dis 31, 3 6.
8. Jones, C.S., Maple, P.A., Andrews, N.J., Paul, I.D. Caul, E.O. (2003) Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection. J Clin Pathol 56, 225 229.
9. Komoda, T. (2007) Kinetic study of antibodies (IgG, IgA) to Chlamydia trachomatis: importance of IgA antibody in screening test for C. trachomatis infection by peptide-based enzyme immunosorbent assay. Jpn J Infect Dis 60, 347 351.
10. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
11. Land, J.A., Gijsen, A.P., Kessels, A.G., Slobbe, M.E. Bruggeman, C.A. (2003) Performance of five serological Chlamydia antibody tests in subfertile women. Hum Reprod 18, 2621 2627.
12. Landis, J.R. Koch, G.G. (1977) An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers. Biometrics 33, 363 374.
13. Maurin, M., Eb, F., Etienne, J. Raoult, D. (1997) Serological cross-reactions between Bartonella and Chlamydia species: implications for diagnosis. J Clin Microbiol 35, 2283 2287.
14. Mitov, I., Haralambieva, I., Petrov, D., Ivanova, R., Kamarinchev, B. Iankov, I. (2003) Cross-reactive monoclonal antibodies raised against the lipopolysaccharide antigen of Salmonella minnesota Re chemotype: diagnostic relevance. Diagn Microbiol Infect Dis 45, 225 231.
15. Morré, S.A., Munk, C., Persson, K., Krüger-Kjaer, S., van Dijk, R., Meijer, C.J. van Den Brule, A.J. (2002) Comparison of three commercially available peptide-based immunoglobulin G (IgG) and IgA assays to microimmunofluorescence assay for detection of Chlamydia trachomatis antibodies. J Clin Microbiol 40, 584 587.
16. Moss, T.R., Darougar, S., Woodland, R.M., Nathan, M., Dines, R.J. Cathrine, V. (1993) Antibodies to Chlamydia species in patients attending a genitourinary clinic and the impact of antibodies to C. pneumoniae and C. psittaci on the sensitivity and the specificity of C. trachomatis serology tests. Sex Transm Dis 20, 61 65.
17. Mygind, P., Christiansen, G., Persson, K. Birkelund, S. (2000) Detection of Chlamydia trachomatis-specific antibodies in human sera by recombinant major outer-membrane protein polyantigens. J Med Microbiol 49, 457 465.
18. Peeling, R.W., Wang, S.P., Grayston, J.T., Blasi, F., Boman, J., Clad, A., Freidank, H., Gaydos, C.A. et al. (2000) Chlamydia pneumoniae serology: interlaboratory variation in microimmunofluorescence assay results. J Infect Dis 181, 426 429.
19. Punnonen, R., Terho, P., Nikkanen, V. Meurman, O. (1979) Chlamydial serology in infertile women by immunofluorescence. Fertil Steril 31, 656 659.
20. Sharma, J., Zhong, Y., Dong, F., Piper, J.M., Wang, G. Zhong, G. (2006) Profiling of human antibody responses to Chlamydia trachomatis urogenital tract infection using microplates arrayed with 156 chlamydial fusion proteins. Infect Immun 74, 1490 1499.
21. Shaw, A.C., Gevaert, K., Demol, H., Hoorelbeke, B., Vandekerckhove, J., Larsen, M.R., Roepstorff, P., Holm, A. et al. (2002) Comparative proteome analysis of Chlamydia trachomatis serovar A, D and L2. Proteomics 2, 164 186.
22. Srugo, I., Gershtein, R., Madjar, S., Elias, I., Tal, J. Nativ, O. (2000) Acute primary Chlamydia trachomatis infection in male adolescents after their first sexual contact. Arch Pediatr Adolesc Med 154, 169 172.
23. Tuuminen, T., Palomäki, P. Paavonen, J. (2000) The use of serologic tests for the diagnosis of chlamydial infections. J Microbiol Methods 42, 265 279.
24. Wagenvoort, J.H., Koumans, D. van de Cruijs, M. (1999) How useful is the Chlamydia micro-immunofluorescence (MIF) test for the gynaecologist? Eur J Obstet Gynecol Reprod Biol 84, 13 15.