Purification and characterization of photosystem I complex from Synechocystis sp. PCC 6803 by expressing histidine-tagged subunits

Biochimica et Biophysica Acta - Bioenergetics

Volumn 1797, Issue 1, 2010, Pages 98-105

  Kubota, Hisako ^a   Sakurai, Isamu ^a   Katayama, Kenta ^a   Mizusawa, Naoki ^a   Ohashi, Shunsuke ^b   Kobayashi, Masami ^b   Zhang, Pengpeng ^c   Aro, Eva Mari ^c   Wada, Hajime ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c UNIVERSITY OF TURKU   (Finland)

Author keywords

Cyanobacteria; Histidine tag; Photosystem I; Purification; Synechocystis sp. PCC 6803

Indexed keywords

CHLOROPHYLL; HISTIDINE; LIPID; MONOMER; PLASTOCYANIN; PROTEIN DERIVATIVE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CONTROLLED STUDY; NONHUMAN; PHOTOAUTOTROPH; PHOTOSYSTEM I; PHOTOSYSTEM II; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SYNECHOCYSTIS; THYLAKOID MEMBRANE;

BASE SEQUENCE; DNA PRIMERS; FERREDOXINS; GENES, BACTERIAL; HISTIDINE; LIPIDS; OLIGOPEPTIDES; OXYGEN CONSUMPTION; PHOTOSYSTEM I PROTEIN COMPLEX; PHOTOSYSTEM II PROTEIN COMPLEX; PIGMENTATION; PROTEIN SUBUNITS; SYNECHOCYSTIS; THYLAKOIDS;

CYANOBACTERIA; SYNECHOCYSTIS SP. PCC 6803;

EID: 71749115086     PISSN: 00052728     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbabio.2009.09.001     Document Type: Article

References (45)

1. Kruip J., Bald D., Boekema E., and Rögner M. Evidence for the existence of trimeric and monomeric photosystem-I complexes in thylakoid membranes from cyanobacteria. Photosyn. Res. 40 (1994) 279-286
2. Jordan P., Fromme P., Witt H.T., Klukas O., Saenger W., and Krau N. Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution. Nature 411 (2001) 909-917
3. Rögner M., Mühlenhoff U., Boekema E.J., and Witt H.T. Mono-, di- and trimeric PS I reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus sp. Size, shape and activity. Biochim. Biophys. Acta 1015 (1990) 415-424
4. Witt I., Witt H.T., Gerken S., Saenger W., Dekker J.P., and Rögner M. Crystallization of reaction center I of photosynthesis-low-concentration crystallization of photoactive protein complexes from the cyanobacterium Synechococcus sp. FEBS Lett. 221 (1987) 260-264
5. Fromme P., and Witt H.T. Improved isolation and crystallization of Photosystem I for structural analysis. Biochim. Biophys. Acta 1365 (1998) 175-184
6. Bricker T.M., Morvant J., Masri N., Sutton H.M., and Frankel L.K. Isolation of a highly active Photosystem II preparation from Synechocystis 6803 using a histidine-tagged mutant of CP 47. Biochim. Biophys. Acta 1409 (1998) 50-57
7. Sugiura M., and Inoue Y. Highly purified thermo-stable oxygen-evolving photosystem II core complex from the thermophilic cyanobacterium Synechococcus elongatus having his-tagged CP43. Plant Cell Physiol. 40 (1999) 1219-1231
8. Roose J.L., Kashino Y., and Pakrasi H.B. The PsbQ protein defines cyanobacterial Photosystem II complexes with highest activity and stability. Proc. Natl. Acad. Sci. U.S.A. 104 (2007) 2548-2553
9. Fey H., Piano D., Horn R., Fischer D., Schmidt M., Ruf S., Schröder W.P., Bock R., and Büchel C. Isolation of highly active photosystem II core complexes with a His-tagged Cyt b559 subunit from transplastomic tobacco plants. Biochim. Biophys. Acta 1777 (2008) 1501-1509
10. Gulis G., Narasimhulu K.V., Fox L.N., and Redding K.E. Purification of His(6)-tagged photosystem I from Chlamydomonas reinhardtii. Photosyn. Res. 96 (2008) 51-60
11. Prodöhl A., Ambill M., El-Mohsnawy E., Lax J., Nowaczyk M., Oworah-Nkruma R., Volkmer T., Wenk S.-O., and Rögner M. Modular device for hydrogen production: optimization of (individual) components. In: Miyake J., Igarashi Y., and Rögner M. (Eds). Biohydrogen III, Renewable Energy System by Biological Solar Energy Conversion (2004), Elsevier, Amsterdam 171-179
12. Tang H.D., and Chitnis P.R. Addition of C-terminal histidyl tags to PsaL and PsaK1 proteins of cyanobacterial photosystem I. Indian J. Biochem. Biophys. 37 (2000) 433-440
13. Grigorieva G., and Shestakov S. Transformation in the cyanobacterium Synechocystis sp. 6803. FEMS Microbiol. Lett. 13 (1982) 367-370
14. Chitnis P.R. Photosystem I: function and physiology. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52 (2001) 593-626
15. Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., and Tabata S. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3 (1996) 109-136
16. Allen M.M. Simple conditions for growth of unicellular blue-green algae on plates. J. Phycol. 4 (1968) 1-4
17. Kashino Y., Lauber W.M., Carroll J.A., Wang Q., Whitmarsh J., Satoh K., and Pakrasi H.B. Proteomic analysis of a highly active photosystem II preparation from the cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel polypeptides. Biochemistry 41 (2002) 8004-8012
18. Sakurai I., Mizusawa N., Ohashi S., Kobayashi M., and Wada H. Effects of the lack of phosphatidylglycerol on the donor side of photosystem II. Plant Physiol. 144 (2007) 1336-1346
19. Gombos Z., Wada H., and Murata N. Direct evaluation of effects of fatty-acid unsaturation on the thermal-properties of photosynthetic activities, as studied by mutation and transformation of Synechocystis PCC 6803. Plant Cell Physiol. 32 (1991) 205-211
20. Pakrasi H.B., Williams J.G., and Arntzen C.J. Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II. EMBO J. 7 (1988) 325-332
21. Hagio M., Gombos Z., Varkonyi Z., Masamoto K., Sato N., Tsuzuki M., and Wada H. Direct evidence for requirement of phosphatidylglycerol in photosystem II of photosynthesis. Plant Physiol. 124 (2000) 795-804
22. Kashino Y., Koike H., and Satoh K. An improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis system for the analysis of membrane protein complexes. Electrophoresis 22 (2001) 1004-1007
23. Blum H., Beier H., and Gross H.J. Improved silver staining of plant-proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8 (1987) 93-99
24. Herranen M., Battchikova N., Zhang P., Graf A., Sirpiö S., Paakkarinen V., and Aro E.M. Towards functional proteomics of membrane protein complexes in Synechocystis sp. PCC 6803. Plant Physiol. 134 (2004) 470-481
25. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
26. Sakurai I., Shen J.R., Leng J., Ohashi S., Kobayashi M., and Wada H. Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants. J. Biochem. 140 (2006) 201-209
27. Bligh E.G., and Dyer W.J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37 (1959) 911-917
28. Wada H., and Murata N. Synechocystis PCC6803 mutants defective in desaturation of fatty acids. Plant Cell Physiol. 30 (1989) 971-978
29. Arnon D.I., McSwain B.D., Tsujimoto H.Y., and Wada K. Photochemical activity and components of membrane preparations from blue-green algae. I. Coexistence of two photosystems in relation to chlorophyll a and removal of phycocyanin. Biochim. Biophys. Acta 357 (1974) 231-245
30. Kobayashi M., Maeda H., Watanabe T., Nakane H., and Satoh K. Chlorophyll-a and β-carotene content in the D1/D2/Cytochrome-b-559 reaction center complex from spinach. FEBS Lett. 260 (1990) 138-140
31. Xu Q., Armbrust T.S., Guikema J.A., and Chitnis P.R. Organization of photosystem I polypeptides (a structural interaction between the PsaD and PsaL subunits). Plant Physiol. 106 (1994) 1057-1063
32. Xu W., Tang H., Wang Y., and Chitnis P.R. Proteins of the cyanobacterial photosystem I. Biochim. Biophys. Acta 1507 (2001) 32-40
33. Fromme P., Jordan P., and Krau N. Structure of photosystem I. Biochim. Biophys. Acta 1507 (2001) 5-31
34. Dühring U., Irrgang K.D., Lünser K., Kehr J., and Wilde A. Analysis of photosynthetic complexes from a cyanobacterial ycf37 mutant. Biochim. Biophys. Acta 1757 (2006) 3-11
35. Dühring U., Ossenbühl F., and Wilde A. Late assembly steps and dynamics of the cyanobacterial photosystem I. J. Biol. Chem. 282 (2007) 10915-10921
36. Battchikova N., and Aro E.M. Cyanobacterial NDH-1 complexes: multiplicity in function and subunit composition. Physiol. Plant. 131 (2007) 22-32
37. Peng L., Shimizu H., and Shikanai T. The chloroplast NAD(P)H dehydrogenase complex interacts with photosystem I in Arabidopsis. J. Biol. Chem. 283 (2008) 34873-34879
38. Sirpiö S., Allahverdiyeva Y., Holmstrom M., Khrouchtchova A., Haldrup A., Battchikova N., and Aro E.M. Novel nuclear-encoded subunits of the chloroplast NAD(P)H dehydrogenase complex. J. Biol. Chem. 284 (2009) 905-912
39. Singh A.K., Li H., and Sherman L.A. Microarray analysis and redox control of gene expression in the cyanobacterium Synechocystis sp. PCC 6803. Physiol. Plant. 120 (2004) 27-35
40. Wegener K.M., Welsh E.A., Thornton L.E., Keren N., Jacobs J.M., Hixson K.K., Monroe M.E., Camp D.G., Smith R.D., and Pakrasi H.B. High sensitivity proteomics assisted discovery of a novel operon involved in the assembly of photosystem II, a membrane protein complex. J. Biol. Chem. 283 (2008) 27829-27837
41. Chitnis V.P., and Chitnis P.R. PsaL subunit is required for the formation of photosystem I trimers in the cyanobacterium Synechocystis sp. PCC 6803. FEBS Lett. 336 (1993) 330-334
42. Schluchter W.M., Shen G., Zhao J., and Bryant D.A. Characterization of psaI and psaL mutants of Synechococcus sp. strain PCC 7002: a new model for state transitions in cyanobacteria. Photochem. Photobiol. 64 (1996) 53-66
43. Vanselow C., Weber A.P., Krause K., and Fromme P. Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria. Biochim. Biophys. Acta 1787 (2009) 46-59
44. Sakurai I., Mizusawa N., Wada H., and Sato N. Digalactosyldiacylglycerol is required for stabilization of the oxygen-evolving complex in photosystem II. Plant Physiol. 145 (2007) 1361-1370
45. Awai K., Watanabe H., Benning C., and Nishida I. Digalactosyldiacylglycerol is required for better photosynthetic growth of Synechocystis sp PCC6803 under phosphate limitation. Plant Cell Physiol. 48 (2007) 1517-1523