Advancement of a multiplex PCR for the differentiation of all currently described Brucella species

Journal of Microbiological Methods

Volumn 80, Issue 1, 2010, Pages 112-114

  Mayer Scholl, Anne ^a   Draeger, Angelika ^a   Göllner, Cornelia ^a   Scholz, Holger C ^b   Nöckler, Karsten ^a  

^a FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^b BUNDESWEHR INSTITUTE OF MICROBIOLOGY   (Germany)

Author keywords

Brucella; Brucellosis; Diagnostics; Multiplex PCR

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; BRUCELLA; BRUCELLA CETI; BRUCELLA INOPATA; BRUCELLA MICROTI; BRUCELLA PINNIPEDIALIS; BRUCELLOSIS; DNA EXTRACTION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; SPECIES DIFFERENTIATION;

BRUCELLA; BRUCELLOSIS; HUMANS; POLYMERASE CHAIN REACTION;

BRUCELLA;

EID: 71749101071     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.10.015     Document Type: Article

References (12)

1. Bricker B.J. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 90 (2002) 435-446
2. Bricker B.J., and Halling S.M. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32 (1994) 2660-2666
3. De B.K., Stauffer L., Koylass M.S., Sharp S.E., Gee J.E., Helsel L.O., Steigerwalt A.G., Vega R., Clark T.A., Daneshvar M.I., Wilkins P.P., and Whatmore A.M. Novel Brucella strain (BO1) associated with a prosthetic breast implant infection. J. Clin. Microbiol. 46 (2008) 43-49
4. Foster G., Jahans K.L., Reid R.J., and Ross H.M. Isolation of Brucella species from cetaceans, seals and an otter. Vet. Rec. 138 (1996) 583-586
5. Garcia-Yoldi D., Marin C.M., de Miguel M.J., Munoz P.M., Vizmanos J.L., and Lopez-Goni I. Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin. Chem. 52 (2006) 779-781
6. Hinic V., Brodard I., Thomann A., Cvetnic Z., Makaya P.V., Frey J., and Abril C. Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J. Microbiol. Methods 75 (2008) 375-378
7. Jahans K.L., Foster G., and Broughton E.S. The characterisation of Brucella strains isolated from marine mammals. Vet. Microbiol. 57 (1997) 373-382
8. Scholz H.C., Hubalek Z., Sedlacek I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kampfer P., Neubauer H., Cloeckaert A., Maquart M., Zygmunt M.S., Whatmore A.M., Falsen E., Bahn P., Gollner C., Pfeffer M., Huber B., Busse H.J., and Nockler K. Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol. 58 (2008) 375-382
9. Scholz H.C., Nöckler K., Göllner C., Bahn P., Vergnaud G., Tomaso H., Al Dahouk S., Kämpfer P., Cloeckaert A., Maquart M., Zygmunt M.S., Whatmore A., Pfeffer M., Huber B., Busse B.-J., and De B.K. Brucella inopinata sp. nov., isolated from a breast implant infection. Int. J. Syst. Evol. Microbiol. (2009)
10. Sohn A.H., Probert W.S., Glaser C.A., Gupta N., Bollen A.W., Wong J.D., Grace E.M., and McDonald W.C. Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp. Emerg. Infect. Dis. 9 (2003) 485-488
11. Velasco J., Romero C., Lopez-Goni I., Leiva J., Diaz R., and Moriyon I. Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int. J. Syst. Bacteriol. 48 Pt 3 (1998) 759-768
12. Verger J.M., Grimont F., Grimont P.A.D., and Grayon M. Brucella, a monospecific genus as shown by deoxyribonucleic-acid hybridization. Int. J. Syst. Bacteriol. 35 (1985) 292-295