Comparison of culture and PCR to detect Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in ear swabs taken from goats

Veterinary Microbiology

Volumn 140, Issue 1-2, 2010, Pages 105-108

  Amores, Joaquín ^a   Corrales, Juan C ^a   Martín, Ángel Gómez ^a   Sánchez, Antonio ^a   Contreras, Antonio ^a   de la Fe, Christian ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Auricular carriers; Contagious agalactia; Culture; Mycoplasma agalactiae; Mycoplasma mycoides subsp. capri; PCR

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EAR INFECTION; EXTERNAL EAR; GOAT; INTERMETHOD COMPARISON; MYCOPLASMA AGALACTIAE; MYCOPLASMA MYCOIDES; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTION; SENSITIVITY AND SPECIFICITY; SPECIES IDENTIFICATION;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; COLONY COUNT, MICROBIAL; EAR; EAR DISEASES; GOAT DISEASES; GOATS; MYCOPLASMA AGALACTIAE; MYCOPLASMA INFECTIONS; MYCOPLASMA MYCOIDES; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

CAPRA HIRCUS; MYCOPLASMA AGALACTIAE; MYCOPLASMA MYCOIDES SUBSP. CAPRI; MYCOPLASMATALES;

EID: 71549154970     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2009.06.036     Document Type: Article

References (27)

1. Bergonier D., Berthelot X., and Poumarat F. Contagious agalactia of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev. Sci. Technol. 16 (1997) 848-873
2. Corrales J., Esnal A., De la Fe C., Sánchez A., Assunçao P., Poveda J., and Contreras A. Contagious agalactia in small ruminants. Small Rumin. Res. 68 (2007) 154-166
3. Cottew G.S., and Yeats F.R. Occurrence of mycoplasmas in clinically normal goats. Aust. Vet. J. 57 (1981) 52-53
4. Cottew G.S., and Yeats F.R. Mycoplasmas and mites in the ears of clinically normal goats. Aust. Vet. J. 59 (1982) 77-81
5. DaMassa A.J. Prevalence of mycoplasmas and mites in the external auditory meatus of goats. Calif. Vet. 12 (1983) 10-17
6. DaMassa A.J., and Brooks D.L. The external ear canal of goats and other animals as a mycoplasma habitat. Small Rumin. Res. 4 (1991) 85-93
7. De la Fe C., Assunçao P., Rosales R.S., Antunes T., and Poveda J.B. Microbiological study of contagious agalactia in a serological endemic area. Vet. J. 170 (2005) 257-259
8. Dedieu L., Mady V., and Lefevre P.C. Development of two PCR assays for the identification of mycoplasmas causing contagious agalactia. FEMS Microbiol. Lett. 129 (1995) 243-249
9. Dorigo-Zetsma J.W., Zaat S.A., Wertheim-van Dillen P.M., Spanjaard L., Rijntjes J., van Waveren G., Jensen J.S., Angulo A.F., and Dankert J. Comparison of PCR, culture, and serological tests for diagnosis of Mycoplasma pneumoniae respiratory tract infection in children. J. Clin. Microbiol. 37 (1999) 14-17
10. Gil M.C., Hermoso de Mendoza M., Rey J., Alonso J.M., Poveda J.B., and Hermoso de Mendoza J. Isolation of mycoplasmas from the external ear canal of goats affected with contagious agalactia. Vet. J. 158 (1999) 152-154
11. Greco G., Corrente M., Martella V., Pratelli A., and Buonavoglia D. A multiplex-PCR for the diagnosis of contagious agalactia of sheep and goats. Mol. Cell. Probes 15 (2001) 21-25
12. Hotzel H., Sachse K., and Pfützner H. A PCR scheme for differentiation of organisms belonging to the Mycoplasma mycoides cluster. Vet. Microbiol. 49 (1996) 31-43
13. Johnson L.R., Drazenovich N.L., and Foley J.E. A comparison of routine culture with polymerase chain reaction technology for the detection of Mycoplasma species in feline nasal samples. J. Vet. Diagn. Invest. 16 (2004) 347-351
14. Luki N., Lebel P., Boucher M., Doray B., Turgeon J., and Brousseau R. Comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. Eur. J. Clin. Microbiol. Infect. Dis. 17 (1998) 255-263
15. Manso-Silván L., Perrier X., and Thiaucourt F. Phylogeny of the Mycoplasma mycoides cluster based on analysis of five conserved protein-coding sequences and possible implications for the taxonomy of the group. Int. J. Syst. Evol. Microbiol. 57 (2007) 2247-2258
16. Mercier P., Pellet M., Morignat E., Calavas D., and Poumarat F. Prevalence of mycoplasmas in external ear canal of goats: influence of the sanitary status of the herd. Small Rumin. Res. 73 (2007) 296-299
17. Peyraud A., Woubit S., Poveda J.B., De la Fe C., Mercier P., and Thiaucourt F. A specific PCR for the detection of Mycoplasma putrefaciens. Mol. Cell. Probes 17 (2003) 289-294
18. Poveda J.B. Biochemical characteristics in mycoplasma identification. In: Miles R., and Nicholas R.A.J. (Eds). Methods in Molecular Biology. Mycoplasma Protocols vol. 104 (1998), Humana Press, Totowa 69-78
19. Poveda J.B., and Nicholas R. Serological identification of mycoplasmas by growth and metabolic inhibition tests. In: Miles R., and Nicholas R.A.J. (Eds). Methods in Molecular Biology. Mycoplasma Protocols vol. 104 (1998), Humana Press, Totowa 105-111
20. Sanguinetti V., and Chiocco D. Contagious agalactia and Mycoplasma mycoides: the situation in Italy. In: Jones G.E. (Ed). Agriculture: Contagious Agalactia and other Mycoplasmal Diseases of Small Ruminants (1987), European Communities, Luxembourg 11-21
21. Spergser J., Aurich C., Aurich J.E., and Rosengarten R. High prevalence of mycoplasmas in the genital tract of asymptomatic stallions in Austria. Vet. Microbiol. 87 (2002) 119-129
22. Stellrecht K.A., Woron A.M., Mishrik N.G., and Venezia R.A. Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas. J. Clin. Microbiol. 42 (2004) 1528-1533
23. Tardy F., Mercier P., Solsona M., Saras E., and Poumarat F. Mycoplasma mycoides subsp. mycoides biotype large colony isolates from healthy and diseased goats: prevalence and typing. Vet. Microbiol. 121 (2007) 268-277
24. Thrusfield M. Diagnostic testing. In: Thrusfield M. (Ed). Veterinary Epidemiology (1995), Blackwell Science Ltd., Oxford 266-284 (Chapter 17)
25. Thrusfield M., Ortega C., de Blas I., Noordhuizen J.P., and Frankena K. Win Episcope 2.0: improved epidemiological software for veterinary medicine. Vet. Rec. 148 (2001) 567-572
26. Tola S., Idini G., Manunta D., Galleri G., Angioi P.P., Rocchigiani A.M., and Leori G. Rapid and specific detection of Mycoplasma agalactiae by polymerase chain reaction. Vet. Microbiol. 51 (1996) 77-84
27. Tola S., Angioi A., Rocchigiani A.M., Idini G., Manunta D., Galleri G., and Leori G. Detection of Mycoplasma agalactiae in sheep milk samples by polymerase chain reaction. Vet. Microbiol. 54 (1997) 17-22