Efficient methodologies for sensitive HIV-1 RNA quantitation from plasma and vaginal secretions

Journal of Clinical Virology

Volumn 46, Issue 4, 2009, Pages 309-313

  Henning, Tara Randolph ^a   Lacour, Nedra ^a   Amedee, Angela Martin ^a  

^a LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Genital; Human immunodeficiency virus; Plasma; Real time PCR; RNAlater

Indexed keywords

VIRUS RNA;

ARTICLE; ASSAY; CLINICAL PROTOCOL; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC ACCURACY; FEMALE GENITAL TRACT INFECTION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; INTERMETHOD COMPARISON; METHODOLOGY; NONHUMAN; PLASMA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; VAGINA SECRETION; VALIDATION STUDY; VIRUS DETECTION;

FEMALE; HIV INFECTIONS; HIV-1; HUMANS; POL GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VAGINA;

EID: 71549148703     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2009.08.015     Document Type: Article

References (22)

1. Kovacs A., Wasserman S.S., Burns D., Wright D.J., Cohn J., Landay A., et al. Determinants of HIV-1 shedding in the genital tract of women. Lancet 358 9293 (2001) 1593-1601
2. Philpott S., Burger H., Tsoukas C., Foley B., Anastos K., Kitchen C., et al. Human immunodeficiency virus type 1 genomic RNA sequences in the female genital tract and blood: compartmentalization and intrapatient recombination. J Virol 79 1 (2005) 353-363
3. Pilcher C.D., Shugars D.C., Fiscus S.A., Miller W.C., Menezes P., Giner J., et al. HIV in body fluids during primary HIV infection: implications for pathogenesis, treatment and public health. AIDS 15 7 (2001) 837-845
4. Quinn T.C., Wawer M.J., Sewankambo N., Serwadda D., Li C., Wabwire-Mangen F., et al. Viral load and heterosexual transmission of human immunodeficiency virus type 1. Rakai Project Study Group. N Engl J Med 342 13 (2000) 921-929
5. Semba R.D., Kumwenda N., Hoover D.R., Taha T.E., Quinn T.C., Mtimavalye L., et al. Human immunodeficiency virus load in breast milk, mastitis, and mother-to-child transmission of human immunodeficiency virus type 1. J Infect Dis 180 1 (1999) 93-98
6. Coombs R.W., Speck C.E., Hughes J.P., Lee W., Sampoleo R., Ross S.O., et al. Association between culturable human immunodeficiency virus type 1 (HIV-1) in semen and HIV-1 RNA levels in semen and blood: evidence for compartmentalization of HIV-1 between semen and blood. J Infect Dis 177 2 (1998) 320-330
7. Fiscus S.A., Brambilla D., Coombs R.W., Yen-Lieberman B., Bremer J., Kovacs A., et al. Multicenter evaluation of methods to quantitate human immunodeficiency virus type 1 RNA in seminal plasma. J Clin Microbiol 38 6 (2000) 2348-2353
8. Benki S., McClelland R.S., Emery S., Baeten J.M., Richardson B.A., Lavreys L., et al. Quantification of genital human immunodeficiency virus type 1 (HIV-1) DNA in specimens from women with low plasma HIV-1 RNA levels typical of HIV-1 nontransmitters. J Clin Microbiol 44 12 (2006) 4357-4362
9. Hofmann-Lehmann R., Swenerton R.K., Liska V., Leutenegger C.M., Lutz H., McClure H.M., et al. Sensitive and robust one-tube real-time reverse transcriptase-polymerase chain reaction to quantify SIV RNA load: comparison of one- versus two-enzyme systems. AIDS Res Hum Retroviruses 16 13 (2000) 1247-1257
10. Hirsch M., and Curran J. Human immunodeficiency viruses. In: Fields B.N., Knipe D.M., and Howley P.M. (Eds). Fields virology. 3rd ed. (1996), Lippincott-Williams, Philadelphia 1953-1976
11. Krol M.A., Olson N.H., Tate J., Johnson J.E., Baker T.S., and Ahlquist P. RNA-controlled polymorphism in the in vivo assembly of 180-subunit and 120-subunit virions from a single capsid protein. Proc Natl Acad Sci USA 96 24 (1999) 13650-13655
12. AMPLICOR/COBAS HIV-1 Monitor Test v1.5 Package Insert. In: Roche Molecular Diagnostics.
13. Hornung R.W., and Reed L.D. Estimation of average concentration in the presence of nondetectable values. Appl Occup Environ Hyg 5 1 (1990) 46-51
14. Sun R., Ku J., Jayakar H., Kuo J.C., Brambilla D., Herman S., et al. Ultrasensitive reverse transcription-PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol 36 10 (1998) 2964-2969
15. Kissinger P., Amedee A., Clark R.A., Dumestre J., Theall K.P., Myers L., et al. Trichomonas vaginalis treatment reduces vaginal HIV-1 shedding. Sex Transm Dis 36 1 (2009) 11-16
16. Abe M., Klett C., Wieland E., Gille S., and Landt O. Two-step real-time PCR quantification of all subtypes of human immunodeficiency virus type 1 by an in-house method using locked nucleic acid-based probes. Folia Med (Plovdiv) 50 3 (2008) 5-13
17. Palmer S., Wiegand A.P., Maldarelli F., Bazmi H., Mican J.M., Polis M., et al. New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol 41 10 (2003) 4531-4536
18. Promso S., Srichunrusami C., Utid K., Lulitanond V., Pairoj W., and Chantratita W. Quantitative detection of human immunodeficiency virus type 1 (HIV-1) viral load by real-time RT-PCR assay using self-quenched fluorogenic primers. Southeast Asian J Trop Med Public Health 37 3 (2006) 477-487
19. Yamamoto N., Tanaka C., Wu Y., Chang M.O., Inagaki Y., Saito Y., et al. Analysis of human immunodeficiency virus type 1 integration by using a specific, sensitive and quantitative assay based on real-time polymerase chain reaction. Virus Genes 32 1 (2006) 105-113
20. Zazzi M., Romano L., Catucci M., Venturi G., De Milito A., and Valensin P.E. Clinical evaluation of an in-house reverse transcription-competitive PCR for quantitation of human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol 37 2 (1999) 333-338
21. Ferns R.B., and Garson J.A. Development and evaluation of a real-time RT-PCR assay for quantification of cell-free human immunodeficiency virus type 2 using a Brome Mosaic Virus internal control. J Virol Methods 135 1 (2006) 102-108
22. Lockey C., Otto E., and Long Z. Real-time fluorescence detection of a single DNA molecule. Biotechniques 24 5 (1998) 744-746