Chapter 20 Protein Precipitation Techniques

Methods in Enzymology

Volumn 463, Issue C, 2009, Pages 331-342

  Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONE; ACID PROTEIN; ALCOHOL; AMMONIUM SULFATE; BASIC PROTEIN; MACROGOL; POLYETHYLENEIMINE; PROTEIN; SOLVENT;

CENTRIFUGATION; FRACTIONATION; HEATING; ISOELECTRIC POINT; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PROTEIN PURIFICATION; REVIEW; SOLUBILITY; ANIMAL; BIOLOGICAL MODEL; CHEMISTRY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; HUMAN; ISOLATION AND PURIFICATION;

AMMONIUM SULFATE; ANIMALS; CHEMICAL PRECIPITATION; HUMANS; LABORATORY TECHNIQUES AND PROCEDURES; MODELS, BIOLOGICAL; POLYETHYLENEIMINE; PROTEINS;

EID: 71549130423     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)63020-2     Document Type: Chapter

References (11)

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2. Burgess R.R., and Jendrisak J.J. A procedure for the rapid, large-scale purification of E. coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14 (1975) 4634-4638
3. Burgess R.R., and Knuth M.W. Purification of a recombinant protein overproduced in E. coli. In: Marshak D., Kadonaga J., Burgess R., Knuth M., Brennan Jr. W., and Lin S.-H. (Eds). Strategies for Protein Purification and Characterization: A Laboratory Manual (1996), Cold Spring Harbor Press, Cold Spring Harbor, NY 219-274
4. Dawson R.M.C., Elliot D.C., Elliot W.H., and Jones K.M. Data for Biochemical Research. 2nd edn. (1969), Oxford University Press, Oxford 616
5. Duellman S.J., and Burgess R.R. Large-scale Epstein-Barr virus EBNA1 protein purification. Protein Expr. Purif. 63 (2008) 128-133
6. Englard S., and Seifter S. Precipitation techniques. Meth. Enzymol. 182 (1990) 287-300
7. Ingham K.C. Precipitation of proteins with polyethylene glycol. Meth. Enzymol. 182 (1990) 301-306
8. Jendrisak J.J. The use of PEI in protein purification. In: Burgess R. (Ed). Protein Purification: Micro to Macro (1987), A.R. Liss, New York 75-97
9. Jendrisak J.J., and Burgess R.R. A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II. Biochemistry 14 (1975) 4639-4645
10. Scopes R.K. Protein Purification, Principles and Practice. 3rd edn. (1994), Springer-Verlag, New York 7-101
11. Zillig W., Zechel K., and Halbwachs H.J. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli. Hoppe Seylers Z. Physiol. Chem. 351 (1970) 221-224