Cryptosporidium oocyst detection in water samples: Floatation technique enhanced with immunofluorescence is as effective as immunomagnetic separation method

Korean Journal of Parasitology

Volumn 47, Issue 4, 2009, Pages 353-357

  Koompapong, Khuanchai ^a   Sutthikornchai, Chantira ^a   Sukthana, Yowalark ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Cryptosporidium; Floatation technique; Immunofluorescence asaay; Immunomagnetic separation; Oocyst; Water

Indexed keywords

RIVER WATER;

ANALYTIC METHOD; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYPTOSPORIDIUM; GIARDIA; IMMUNOFLUORESCENCE; IMMUNOMAGNETIC SEPARATION; INTERMETHOD COMPARISON; NONHUMAN; OOCYST; PH; SEPARATION TECHNIQUE; SUCROSE FLOATATION; TEMPERATURE; TURBIDITY; WATER ANALYSIS; WATER CONTAMINATION; WATER SAMPLING;

ANIMALS; CRYPTOSPORIDIUM; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOMAGNETIC SEPARATION; OOCYSTS; PARASITOLOGY; SENSITIVITY AND SPECIFICITY; WATER;

CRYPTOSPORIDIUM; GIARDIA; PROTOZOA;

EID: 71549121684     PISSN: 00234001     EISSN: 17380006     Source Type: Journal    
DOI: 10.3347/kjp.2009.47.4.353     Document Type: Article

References (18)

1. Feng YY, Ong SL, Hu JY, Song LF, Tan XL, Ng WJ. Effect of particles on the recovery of Cryptosporidium oocysts from source water samples of various turbidities. Appl Environ Microbiol 2003; 69: 1898-1903.
2. Kuczynska E, Boyer DG, Shelton DR. Comparison of immunofluorescence assay and immunomagnetic electrochemiluminescence in detection of Cryptosporidium parvum oocysts in karst water samples. J Microbiol Methods 2003; 53: 17-26.
3. Fujino T, Matsuo T, Okada M, Matsui T. Detection of a small number of Cryptosporidium parvum oocysts by sugar floatation and sugar centrifugation methods. J Vet Med Sci 2006; 68: 1191-1193.
4. LeChevallier MW, Di Giovanni GD, Clancy JL, Bukhari Z, Bukhari S, Rosen JS, Sobrinho J, Frey MM. Comparison of method 1623 and cell culture-PCR for detection of Cryptosporidium spp. in source waters. Appl Environ Microbiol 2003; 69: 971-979.
5. Miller WA, Lewis DJ, Pereira MD, Lennox M, Conrad PA,Tate KW, Atwill ER. Farm factors associated with reducing Cryptosporidium loading in storm runoff from dairies. J Environ Qual 2008; 37: 1875-1882.
6. Downey AS, Graczyk TK. Maximizing recovery and detection of Cryptosporidium parvum oocysts from spiked eastern oyster (Crassostrea virginica) tissue samples. Appl Environ Microbiol 2007; 73: 6910-6915.
7. Sacco C, Bianchi M, Lorini C, Burrini D, Berchielli S, Lanciotti E. [Removal of Cryptosporidium and Giardia in drinking water treatment in a Tuscan area]. Ann Ig 2006; 18: 117-126.
8. Iacovski RB, Barardi CR, Simoes CM. Detection and enumeration of Cryptosporidium sp. oocysts in sewage sludge samples from the city of Floriano@polis (Brazil) by using immunomagnetic separation combined with indirect immunofluorescence assay. Waste Manag Res 2004; 22: 171-176.
9. Chesnot T, Schwartzbrod J. Quantitative and qualitative comparison of density-based purification methods for detection of Cryptosporidium oocysts in turbid environmental matrices. J Microbiol Methods 2004; 58: 375-386.
10. Ware MW, Wymer L, Lindquist HD, Schaefer FW3rd. Evaluation of an alternative IMS dissociation procedure for use with Method 1622: Detection of Cryptosporidium in water. J Microbiol Methods 2003; 55: 575-583.
11. Bonadonna L, Briancesco R, Ottaviani M, Veschetti E. Occurrence of Cryptosporidium oocysts in sewage effluents and correlation with microbial, chemical and physical water variables. Environ Monit Assess 2002; 75: 241-252.
12. Campbell A, Smith H. Immunomagnetic separation of Cryptosporidium oocysts from water samples: Round robin comparison of techniques. Water Sci Technol 1997; 35: 397-401.
13. McNabb SJ, Hansel DM, Welch DF, Heijbel H, McKee GL, Istre GR. Comparison of sedimentation and floatation techniques for identification of Cryptosporidium sp. oocysts in a large outbreak of human diarrhea. J Clin Microbiol 1985; 22: 587-589.
14. Muchiri JM, Ascolillo L, Mugambi M, Mutwiri T, Ward HD, Naumova EN, Egorov AI, Cohen S, Else JG, Griffiths JK. Seasonality of Cryptosporidium oocyst detection in surface waters of Meru, Kenya as determined by two isolation methods followed by PCR. J Water Health 2009; 7: 67-75.
15. Kuhn RC, Rock CM, Oshima KH. Effect of pH and magnetic material on immunomagnetic separation of Cryptosporidium oocysts from concentrated water samples. Appl Environ Microbiol 2002; 68: 2066-2070.
16. Yakub GP, Stadterman-Knauer KL. Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodinalis from high-iron matrices. Appl Environ Microbiol 2000; 66: 3628-3631.
17. Rodgers MR, Flanigan DJ, Jakubowski W. Identification of algae which interfere with the detection of Giardia cysts and Cryptosporidium oocysts and a method for alleviating this interference. Appl Environ Microbiol 1995; 61: 3759-3763.
18. Sutthikornchai C, Jantanavivat C, Thongrungkiat S, Harnroongroj T, Sukthana Y. Protozoal contamination of water used in Thai frozen food industry. Southeast Asian J Trop Med Public Health 2005; 36(suppl): 41-45.