High-level expression of soluble subunit b of F1F0 ATP synthase in Escherichia coli cell-free system

Applied Microbiology and Biotechnology

Volumn 85, Issue 2, 2009, Pages 303-311

  Lian, Jiazhang ^a   Ma, Yi ^a,b   Cai, Jin ^a   Wu, Ming ^a   Wang, Jufang ^b   Wang, Xiaoning ^b   Xu, Zhinan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

ATP synthase; Cell free expression system; Escherichia coli; Membrane protein; Subunit b of ATP synthase

Indexed keywords

AS PRECIPITATES; ATP SYNTHASE; CELL-FREE EXPRESSION; CELL-FREE SYSTEM; CONCENTRATION OF; E. COLI; ESCHERICHIA COLI CELLS; EXPRESSION LEVELS; HIGH LEVEL EXPRESSION; MEMBRANE PROTEINS; NANO ELECTROMECHANICAL SYSTEMS; OVER-EXPRESSION; POLYMER VESICLE; RESOLUBILIZATION; SYNTHASES;

ADENOSINETRIPHOSPHATE; BIOCHEMISTRY; CRITICAL MICELLE CONCENTRATION; ENZYMES; ESCHERICHIA COLI; MEMBRANES; MICELLES; SOAPS (DETERGENTS); TOXIC MATERIALS;

PROTEINS;

DETERGENT; LIPOSOME; MEMBRANE PROTEIN; POLYMER; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE;

BIOTECHNOLOGY; COLIFORM BACTERIUM; DETERGENT; ENZYME ACTIVITY; NANOTECHNOLOGY; PLASMID; PROTEIN; SOLUBILIZATION; TOXIC SUBSTANCE; TOXICITY;

ARTICLE; BACTERIAL CELL; BETA CHAIN; BIOSYNTHESIS; CELL FREE SYSTEM; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; ESCHERICHIA COLI; MICELLE; NONHUMAN; PROTEIN EXPRESSION; SOLUBILIZATION;

BACTERIAL PROTON-TRANSLOCATING ATPASES; CELL-FREE SYSTEM; DETERGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 71349088706     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2055-z     Document Type: Article

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