Quantification of viable Candidatus Liberibacter asiaticus in hosts using quantitative PCR with the aid of ethidium monoazide (EMA)

European Journal of Plant Pathology

Volumn 124, Issue 4, 2009, Pages 553-563

  Trivedi, P ^a   Sagaram, U S ^a   Kim, J S ^a   Brlansky, R H ^a   Rogers, M E ^a   Stelinski, L L ^a   Oswalt, C ^b   Wang, N ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

Candidatus Liberibacter asiaticus; Citrus greening; Ethidium monoazide; Quantitative PCR

Indexed keywords

BACTERIAL DISEASE; BACTERIUM; DNA; EPIDEMIOLOGY; INSECT; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; SYMPTOM;

BACTERIA (MICROORGANISMS); CANDIDATUS LIBERIBACTER; CANDIDATUS LIBERIBACTER ASIATICUS; CATHARANTHUS ROSEUS; CITRUS; DIAPHORINA CITRI; NEGIBACTERIA; PSYLLIDAE;

EID: 71349086082     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10658-009-9439-x     Document Type: Article

References (37)

1. Aldaghi, M., Massart, S., Roussel, S., & Jijakli, M. H. (2006). Establishment of a new method for a rapid and precise estimation of apple proliferation phytoplasma concentration in periwinkle. Communications in Agricultural and Applied Biological Sciences, 71, 853-857.
2. Alvarez, A. M. (2004). Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases. Annual Review of Phytopathology, 42, 339-366. doi: 10. 1146/annurev. phyto. 42. 040803. 140329.
3. Bach, H.-J., Tomanova, J., Schloter, M., & Munch, J. C. (2002). Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification. Journal of Microbiological Methods, 49, 235-245. doi: 10. 1016/S0167-7012(01)00370-0.
4. Blomquist, C. L., & Kirkpatrick, B. C. (2002). Identification of phytoplasma strains and insect vectors of peach yellow leaf roll disease in California. Plant Disease, 86, 759-763. doi: 10. 1094/PDIS. 2002. 86. 7. 759.
5. Bové, J. M. (2006). Huanglongbing: A destructive, newly emerging, century-old disease of citrus. Journal of Plant Pathology, 88, 7-37.
6. Christensen, N. M., Nicolaisen, M., Hansen, M., & Schulz, A. (2004). Distribution of phytoplasmas in infected plants as revealed by real-time PCR and bioimaging. Molecular Plant-Microbe Interactions, 17, 1175-1184. doi: 10. 1094/MPMI. 2004. 17. 11. 1175.
7. Dahllöf, I., Baillie, H., & Kjelleberg, S. (2000). rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity. Applied and Environmental Microbiology, 66, 3376-3380. doi: 10. 1128/AEM. 66. 8. 3376-3380. 2000.
8. Garnier, M., & Bové, J. M. (1983). Transmission of the organism associated with citrus greening disease from sweet orange to periwinkle by dodder. Phytopathology, 73, 1358-1363. doi: 10. 1094/Phyto-73-1358.
9. Garnier, M., & Bové, J. M. (1993). Citrus greening disease and the greening bacterium. In P. Moreno, J. V. daGraca, & L. W. Timmer (Eds.), Proceedings of 12th conference of international organization of citrus virologists (pp. 212-219). Riverside, CA: IOCV.
10. Halbert, S. E., & Manjunath, K. L. (2004). Asian citrus psyllids (Sternorryhncha: Psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida. The Florida Entomologist, 87, 330-352. doi: 10. 1653/0015-4040(2004)087[0330: ACPSPA]2. 0. CO; 2.
11. Hill, B. L., & Purcell, A. H. (1997). Populations of Xylella fastidiosa in plants required for transmission by an efficient vector. Phytopathology, 87, 1197-1201. doi: 10. 1094/PHYTO. 1997. 87. 12. 1197.
12. Hocquellet, A., Bové, J. M., & Garnier, M. (1997). Production and evaluation of non-radioactive probes for the detection of the two Candidatus Liberobacter species associated with citrus huanglongbing (Greening). Molecular and Cellular Probes, 11, 433-438. doi: 10. 1006/mcpr. 1997. 0140.
13. Hocquellet, A., Toorawa, P., Bové, J. M., & Garnier, M. (1999). Detection and identification of the two Candidatus Liberobacter species associated with citrus huanglongbing by PCR amplification of ribosomal protein genes of the β operon. Molecular and Cellular Probes, 13, 373-379. doi: 10. 1006/mcpr. 1999. 0263.
14. Jagoueix, S., Bové, J. M., & Garnier, M. (1994). The phloem-limited bacterium of greening disease of citrus is a member of alpha subdivision of the Proteobacteria. International Journal of Systematic Bacteriology, 44, 379-386.
15. Jarausch, W., Lansac, M., Saillard, C., Broquaire, J. M., & Dosba, F. (1998). PCR assay for specific detection of European stone fruit yellows phytoplasmas and its use for epidemiological studies in France. European Journal of Plant Pathology, 104, 17-27. doi: 10. 1023/A: 1008600828144.
16. Josephson, K. L., Gerba, C. P., & Pepper, I. L. (1993). Polymerase chain reaction detection of nonviable bacterial pathogens. Applied and Environmental Microbiology, 59, 3513-3515.
17. Kawabe, K., Truc, N. T. N., Lan, B. T. N., Hong, L. T. T., & Onuki, M. (2006). Quantification of DNA of citrus huanglongbing pathogen in diseased leaves using competitive PCR. Journal of General Plant Pathology, 72, 355-359. doi: 10. 1007/s10327-006-0306-8.
18. Koonjul, P. K., Brandt, W. F., Farrant, J. M., & Lindsey, G. G. (1999). Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA. Nucleic Acids Research, 27, 915-916. doi: 10. 1093/nar/27. 3. 915.
19. Li, W. B., Pria Jr, W. D., Lacava, P. M., Qin, X., & Hartung, J. S. (2003). Presence of Xylella fastidiosa in sweet orange fruit and seeds and its transmission to seedlings. Phytopathology, 93, 953-958. doi: 10. 1094/PHYTO. 2003. 93. 8. 953.
20. Li, W. B., Hartung, J. S., & Levy, L. (2006). Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing. Journal of Microbiological Methods, 66, 104-115. doi: 10. 1016/j. mimet. 2005. 10. 018.
21. Lin, S., Zhang, H., & Dubois, A. (2006). Wide distribution and low abundance of Pfiesteria piscicida as detected by mtDNA-18S rDNA real-time PCR. Journal of Plankton Research, 28, 667-681. doi: 10. 1093/plankt/fbi150.
22. Luo, L. X., Wllters, C., Bolkan, H., Liu, X. L., & Li, J. Q. (2008). Quantification of viable cells of Clavibacter michiganensis subsp. michiganensis using a DNA binding dye and a real time PCR assay. Plant Pathology, 57, 332-337. doi: 10. 1111/j. 1365-3059. 2007. 01736. x.
23. Morrison, T. B., Weiss, J. J., & Wittwer, C. T. (1999). Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. BioTechniques, 24, 954-962.
24. Nocker, A., & Camper, A. K. (2006). Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Applied and Environmental Microbiology, 72, 1997-2004. doi: 10. 1128/AEM. 72. 3. 1997-2004. 2006.
25. Norton, D. M., & Batt, C. A. (1999). Detection of viable Listeria monocytogenes with a 5′ nuclease PCR assay. Applied and Environmental Microbiology, 65, 2122-2127.
26. Rainey, F. A., Ward-Rainey, N. L., Janssen, P. H., & Hippe, H. (1996). Clostridium paradoxum DSM 7308(T) contains multiple 16S rRNA genes with heterogeneous intervening sequences. Microbiology, 142, 2087-2095.
27. Renz, K. G., Islam, A., Cheetham, B. F., & Walkden-Brown, S. W. (2006). Absolute quantification using real-time polymerase chain reaction of Marek's disease virus serotype 2 in field dust samples, feather tips and spleens. Journal of Virological Methods, 135, 186-191. doi: 10. 1016/j. jviromet. 2006. 03. 017.
28. Rudi, K., Moen, B., Drømtrop, S. M., & Holck, A. L. (2005). Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Applied and Environmental Microbiology, 71, 1018-1024. doi: 10. 1128/AEM. 71. 2. 1018-1024. 2005.
29. Salm, H., & Geider, K. (2004). Real-time PCR for detection and quantification of Erwinia amylovora, the causal agent of fireblight. Plant Pathology, 53, 602-610. doi: 10. 1111/j. 1365-3059. 2004. 01066. x.
30. SAS Institute (2004). The SAS system for Windows. Release 9. 1. Cary, NC: SAS Institute.
31. Soejima, T., Lida, K. I., Qin, T., Taniai, H., Seki, M., Takade, A., et al. (2007). Photoactivated ethidium monoazide directly cleaves bacterial DNA and is applied to PCR for discrimination of live and dead bacteria. Microbiology and Immunology, 51, 763-775.
32. Tatineni, S., Sagaram, U. S., Gowda, S., Robertson, C. J., Dawson, W. O., Iwanami, T., et al. (2008). In Planta distribution of 'Candidatus Liberibacter asiaticus' as revealed by polymerase chain reaction (PCR) and real-time PCR. Phytopathology, 98, 592-599. doi: 10. 1094/PHYTO-98-5-0592.
33. Teixeira, D. C., Saillard, C., Couture, C., Martins, E. C., Wulff, N. A., Eveillard-Jagoueix, S., et al. (2008). Distribution and quantification of Candidatus Liberibacter americanus agent of huanglongbing disease of citrus in Sao Paulo State, Brasil, in leaves of an affected sweet orange tree as determined by PCR. Molecular and Cellular Probes, 22, 139-150. doi: 10. 1016/j. mcp. 2007. 12. 006.
34. Wagner, A. O., Malin, C., Knapp, B. A., & Illmer, P. (2008). Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide. Applied and Environmental Microbiology, 74, 2537-2539. doi: 10. 1128/AEM. 02288-07.
35. Wang, S., & Levin, R. E. (2006). Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR. Journal of Microbiological Methods, 64, 1-8. doi: 10. 1016/j. mimet. 2005. 04. 023.
36. Wang, Z., Yin, Y., Hu, H., Yuan, Q., Peng, G., & Xia, Y. (2006). Development and application of molecular-based diagnosis for 'Candidatus Liberibacter asiaticus', the causal pathogen of citrus huanglongbing. Plant Pathology, 55, 630-638. doi: 10. 1111/j. 1365-3059. 2006. 01438. x.
37. Zhang, H., & Lin, S. (2005). Development of a cob-18S rRNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment. Applied and Environmental Microbiology, 71, 7053-7063. doi: 10. 1128/AEM. 71. 11. 7053-7063. 2005.