Assessment of metabolic capabilities of PLHC-1 and RTL-W1 fish liver cell lines

Cell Biology and Toxicology

Volumn 25, Issue 6, 2009, Pages 611-622

  Thibaut, Rémi ^a   Schnell, Sabine ^a   Porte, Cinta ^a  

^a DEPARTMENT OF ENVIRONMENTAL CHEMISTRY   (Spain)

Author keywords

Fish cell lines; Metabolic pathways; PLHC 1; RTL W1

Indexed keywords

1 NAPHTHOL; 3BETA ANDROSTANEDIOL; ANDROSTANOLONE; ANDROSTENEDIONE; CYTOCHROME P450 1A; CYTOCHROME P450 3A; GLUTATHIONE TRANSFERASE; HYDROXYSTEROID DEHYDROGENASE; LAURIC ACID; STEROID REDUCTASE; TESTOSTERONE;

ANIMAL CELL; ARTICLE; BIOTRANSFORMATION; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; GLUCURONIDATION; HORMONE METABOLISM; HYDROXYLATION; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; SULFATION; XENOBIOTIC METABOLISM;

ANIMALS; BIOTRANSFORMATION; CELL LINE; CYTOCHROME P-450 ENZYME SYSTEM; FUNDULIDAE; HEPATOCYTES; LAURIC ACIDS; METABOLIC NETWORKS AND PATHWAYS; NAPHTHOLS; ONCORHYNCHUS MYKISS; SUBCELLULAR FRACTIONS; TESTOSTERONE; TOXICITY TESTS;

EID: 71349083783     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10565-008-9116-4     Document Type: Article

References (52)

1. A Arukwe R Thibaut K Ingebrigtsen T Celius A Goksøyr JP Cravedi 2000 In vivo and in vitro metabolism and organ distribution of nonylphenol in Atlantic salmon (Salmo salar) Aquat Toxicol 49 289 304 10.1016/S0166-445X(99) 00084-3 10854672 (Pubitemid 30347387)
2. SM Baksi JM Frazier 1990 Isolated fish hepatocytes-model systems for toxicology research Aquat Toxicol 16 229 56 10.1016/0166-445X(90)90039-R (Pubitemid 20151223)
3. H Babich DW Rosenberg E Borenfreund 1991 In vitro cytotoxicity studies with the fish hepatoma cell line, PLHC-1 (Poeciliopsis lucida) Ecotoxicol Environ Saf 21 327 36 10.1016/0147-6513(91)90072-W 1868789
4. ME Baker 2004 Co-evolution of steroidogenic and steroid-inactivating enzymes and adrenal and sex steroid receptors Mol Cell Endocrinol 215 55 62 10.1016/j.mce.2003.11.007 15026175 (Pubitemid 38333243)
5. NC Bols K Schirmer EM Joyce DG Dixon JJ Greenberg Whyte 1999 Ability of polycyclic aromatic hydrocarbons to induce 7-ethoxyresorufin-o-deethylase activity in a trout liver cell line Ecotoxicol Environ Saf 44 118 28 10.1006/eesa.1999.1808 10499998 (Pubitemid 29446298)
6. Bols NC, Dayeh VR, Lee LEJ, Schirmer K. Use of fish cell lines in toxicology of fish. In: Moon TW, Mommsen TP, editors. Biochemistry and molecular biology of fishes-environmental toxicology. vol 6. Amsterdam: Elsevier Science; 2005. p. 43-84.
7. DR Buhler JL Wang-Buhler 1998 Rainbow trout cytochrome P450s: purification, molecular aspects, metabolic activity, induction and role in environment monitoring Comp Biochem Phys C 121 107 37
8. A Castaño NC Bols T Braunbeck P Dierickx M Halder B Isomaa K Kawahara LEJ Lee C Mothersill P Pärt G Repetto JR Sintes H Rufli R Smith C Wood H Segner 2003 The use of fish cells in ecotoxicology. The report and recommendations of ECVAM workshop 47 ATLA 31 317 51 15612875
9. M Celander M Hahn JJ Stegeman 1996 Cytochromes P450 (CYP) in the Poeciliopsis lucida hepatocellular carcinoma cell line (PLHC-1): dose- and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A Arch Biochem Biophys 329 113 22 10.1006/abbi.1996.0198 8619627
10. DJ Clark SG George B Burchell 1991 Glucuronidation in fish Aquat Toxicol 20 35 56 10.1016/0166-445X(91)90040-G
11. JP Cravedi A Paris E Perdu-Durand P Prunet 1995 Influence of growth hormone on the hepatic mixed function oxidase and transferase systems of rainbow trout Fish Physiol Biochem 14 259 66 10.1007/BF00004064
12. JP Cravedi A Paris G Monod A Devaux G Flouriot Y Valotaire 1996 Maintenance of cytochrome P450 content and phase I and phase II enzyme activities in trout hepatocytes cultured as spheroidal aggregates Comp Biochem Phys C 113 241 6
13. JP Cravedi E Perdu-Durand A Paris 1998 Cytochrome P450-dependent metabolic pathways and glucuronidation in trout liver slices Comp Biochem Phys C 121 267 75
14. JP Cravedi 2002 Role of biotransformation in the fate and toxicity of chemicals: consequences for the assessment of residues in fish Rev Med Vet (Toulouse) 153 419 24
15. K Fent 2001 Fish cell lines as versatile tools in ecotoxicology: assessment of cytotoxicity, cytochrome P4501A induction potential and estrogenic activity of chemicals and environmental samples Toxicol In Vitro 15 477 88 10.1016/S0887-2333(01)00053-4 11566581 (Pubitemid 32889676)
16. N Gamage A Barnett N Hempel RG Duggleby KF Windmill JL Martin ME McManus 2006 Human sulfotransferases and their role in chemical metabolism Toxicol Sci 90 5 22 10.1093/toxsci/kfj061 16322073 (Pubitemid 43264722)
17. MJ Gómez-Lechón MT Donato JV Castell R Jover 2003 Human hepatocytes as a tool for studying toxicity and drug metabolism Curr Drug Metab 4 292 312 10.2174/1389200033489424 12871046 (Pubitemid 36958330)
18. ME Hahn L Bonnie J Wood ward JJ Stegeman SW Kennedy 1996 Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multiwell plates: response to TCDD, TCDF, and two planar PCBs Environ Toxicol Chem 15 582 91 10.1897/1551-5028(1996)015<0582:RAOICP>2.3. CO;2 (Pubitemid 26110333)
19. LE Hightower JL Renfro 1988 Recent applications of fish cell culture to biomedical research J Exp Zool 248 290 302 10.1002/jez.1402480307 3062124 (Pubitemid 19016512)
20. SE Huuskonen A Tuvikene M Trapido K Fent ME Hahn 2000 Cytochrome P4501A induction and porphyrin accumulation in PLHC-1 cells exposed to sediment and oil shale extracts Arch Environ Contam Toxicol 38 59 69 10.1007/s002449910008 10556372
21. MO James 1987 Conjugation of organic pollutants in aquatic species Environ Health Perspect 71 97 103 10.2307/3430417 3297669
22. LEJ Lee JH Clemons DG Bechtel SJ Caldwell KB Han M Pasitschniak-Arts DD Mosser NC Bols 1993 Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity Cell Biol Toxicol 9 279 94 10.1007/BF00755606 8299006 (Pubitemid 23304328)
23. I Leguen C Carlsson E Perdu-Durand P Prunet P Pärt JP Cravedi 2000 Xenobiotic and steroid biotransformation activities in rainbow trout gill epithelial cells in culture Aquat Toxicol 48 165 76 10.1016/S0166-445X(99)00043- 0 10686323 (Pubitemid 30109209)
24. A Lorenzen SA Kennedy 1993 A fluorescence-based protein assay for use with a microplate reader Anal Biochem 214 346 8 10.1006/abio.1993.1504 8250247
25. V Luu-The 2001 Analysis and characteristics of multiple types of human 17b-hydroxysteroid dehydrogenases J Steroid Biochem 76 143 51 10.1016/S0960-0760(00)00155-2 (Pubitemid 32510676)
26. R Martin-Skilton R Lavado R Thibaut C Minier C Porte 2006 Evidence of endocrine alteration in the red mullet, Mullus barbatus from the NW Mediterranean Environ Pollut 141 60 8 10.1016/j.envpol.2005.08.016 16203071
27. R Martin-Skilton MWH Coughtrie C Porte 2006 Sulfotransferase activities towards xenobiotics and estradiol in two marine fish species (Mullus barbatus and Lepidorhombus boscii): characterization and inhibition by endocrine disrupters Aquat Toxicol 79 24 30 10.1016/j.aquatox.2006.04.012 16806523 (Pubitemid 44066584)
28. CP Martínez-Jiménez R Jover MJ Gómez-Lechón JV Castell 2004 Can hepatoma cell lines be redifferentiated to be used in drug metabolism studies ATLA-Altern Lab Anim 32 65 74
29. CP Martínez-Jiménez MJ Gómez-Lechón JV Castell R Jover 2006 Engineering human hepatoma cells with key transcription factors to generate metabolically competent hepatic models ALTEX-Altern Therexp 23 435 40
30. H Morrison P Young S George 1985 Conjugation of organic compounds in isolated hepatocytes from a marine fish, the plaice, Pleuronectes platessa Biochem Pharmacol 34 3933 9 10.1016/0006-2952(85)90447-2 4062967 (Pubitemid 16195466)
31. DL Nabb RT Mingoia CH Yang X Han 2006 Comparison of basal level metabolic enzyme activities of freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat Aquat Toxicol 80 52 9 10.1016/j.aquatox.2006.07. 012 16935359 (Pubitemid 44416467)
32. S Nehls H Segner 2001 Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the comet assay Environ Toxicol 16 321 9 10.1002/tox.1039 11501281 (Pubitemid 32734205)
33. TM Penning 1997 Molecular endocrinology of hydroxysteroid dehydrogenases Endocr Rev 18 281 305 10.1210/er.18.3.281 9183566 (Pubitemid 27246879)
34. C Rodríguez-Antona MT Donato A Boobis RJ Edwards PS Watts JV Castell MJ Gómez-Lechón 2002 Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells Xenobiotica 32 505 20 10.1080/00498250210128675 12160483 (Pubitemid 34743622)
35. M Sadar TB Andersson 2001 Regulation of cytochrome P450 in a primary culture of rainbow trout hepatocytes In Vitro Cell Dev Biol-An 37 180 184 10.1290/1071-2690(2001)037<0180:ROCPIA>2.0.CO;2 (Pubitemid 32427689)
36. EG Schuetz SA Wrighton JL Barwick PS Guzelian 1984 Induction of cytochrome P-450 by glucocorticoids in rat liver. I. Evidence that glucocorticoids and pregnenolone 16 alpha-carbonitrile regulate de novo synthesis of a common form of cytochrome P-450 in cultures of adult rat hepatocytes and in the liver in vivo J Biol Chem 259 1999 2006 6141167
37. EG Schuetz JD Schuetz SC Strom MT Thompson RA Fisher DT Molowa D Li PS Guzelian 1993 Regulation of human liver cytochromes P-450 in family 3A in primary and continuous culture of human hepatocytes Hepathology 18 1254 62 10.1002/hep.1840180535
38. Segner H. Fish cell lines as a tool in aquatic toxicology. In: Braunbeck T, Hinton DE, Streit B, editors. Fish Ecotoxicology. Basel, Switzerland: Birkhäuser; 1998. p. 1-38.
39. H Segner C Chesne JP Cravedi B Fauconneau D Houlihan F LeGac M Loir C Mothersill P Pärt Y Valotaire 2001 Cellular approaches for diagnostic effects assessment in ecotoxicology: introductory remarks to an EU-funded project Aquat Toxicol 53 153 8 10.1016/S0166-445X(01)00162-X 11408076 (Pubitemid 32530078)
40. H Segner 2004 Cytotoxycity assays with fish cells as an alternative to the acute lethality test with fish ATLA-Altern Lab Anim 32 375 82
41. Y Singh JB Cooke DE Hinton MG Miller 1996 Trout liver slices for metabolism and toxicity studies Drug Metab Dispos 24 7 14 8825184
42. JMW Smeets A Voormolen DE Tillitt J Everaarts W Seinen M van den Berg 1999 Cytochrome P4501A induction, benzo[a]pyrene metabolism, and nucleotide adduct formation in fish hepatoma cells: effect of preexposure to 3,3′,4,4′,5-pentachlorobiphenyl Environ Toxicol Chem 18 474 80 10.1897/1551-5028(1999)018<0474:CPIBAP>2.3.CO;2 (Pubitemid 29091737)
43. JMW Smeets J Wamsteker B Roth J Everaarts M van den Berg 2002 Cytochrome P4501A induction and testosterone hydroxylation in cultured hepatocytes of four fish species Chemosphere 46 163 72 10.1016/S0045-6535(01)00054-6 11831221 (Pubitemid 34072358)
44. TA Smolarek SL Morgan CG Moynihan H Lee RG Harvey W Baird 1987 Metabolism and DNA adduct formation of benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene in fish cell lines in culture Carcinogenesis 8 1501 9 10.1093/carcin/8.10.1501 3115618
45. A Sturm JP Cravedi E Perdu M Baradat H Segner 2001 Effects of prochloraz and nonylphenol diethoxylate on hepatic biotransformation enzymes in trout: a comparative in vitro/in vivo-assessment using cultured hepatocytes Aquat Toxicol 53 229 45 10.1016/S0166-445X(01)00168-0 11408082 (Pubitemid 32530083)
46. R Thibaut L Debrauwer D Rao JP Cravedi 1998 Characterization of biliary metabolites of 4-n-nonylphenol in rainbow trout (Oncorhynchus mykiss) Xenobiotica 28 745 57 10.1080/004982598239164 9741953
47. R Thibaut L Debrauwer E Perdu A Goksøyr JP Cravedi A Arukwe 2002 Regio-specific hydroxylation of nonylphenol and the involvement of CYP2K- and CYP2M-like iso-enzymes in Atlantic salmon (Salmo salar) Aquat Toxicol 56 177 90 10.1016/S0166-445X(01)00204-1 11792434 (Pubitemid 34041556)
48. R Thibaut C Porte 2004 Effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish J Steroid Biochem 92 485 94 10.1016/j.jsbmb.2004.10.008 (Pubitemid 40187166)
49. R Thibaut S Schnell C Porte 2006 The interference of pharmaceuticals with endogenous and xenobiotic metabolizing enzymes in carp liver: an in-vitro study Environ Sci Technol 40 5154 60 10.1021/es0607483 16955921 (Pubitemid 44257486)
50. R Thibaut C Porte 2008 Effects of fibrates, anti-inflammatory drugs and antidepressants in the fish hepatoma cell line PLHC-1: cytotoxicity and interactions with cytochrome P450 1A Toxicol In Vitro 22 1128 35 10.1016/j.tiv.2008.02.020 18406567
51. Z Tong MO James 2000 Purification and characterization of hepatic and intestinal phenol sulfotransferase with high affinity for benzo[a]pyrene phenols from channel catfish, Ictalurus punctatus Arch Biochem Biophys 376 409 19 10.1006/abbi.2000.1746 10775429 (Pubitemid 30210890)
52. JD Wilson 1999 The role of androgens in male gender role behavior Endocr Rev 20 726 37 10.1210/er.20.5.726 10529900