Improvement of DNA/Metal particle adsorption in tungsten-based biolistic bombardment; alkaline pH is necessary for DNA adsorption and suppression of DNA degradation

Journal of Plant Biology

Volumn 52, Issue 6, 2009, Pages 524-532

  Yoshimitsu, Yuya ^a   Tanaka, Kiwamu ^a   Tagawa, Takashi ^a   Nakamura, Yasushi ^b   Matsuo, Tomoaki ^a   Okamoto, Shigehisa ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Alkaline pH; Biolistic bombardment; DNA adsorption; DNA degradation; Tungsten particle

Indexed keywords

EID: 71249160931     PISSN: 12269239     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12374-009-9068-0     Document Type: Article

References (19)

1. Breitler JC, Labeyrie A, Meynard D, Legavre T, Guiderdoni E (2002) Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes. Theor Appl Genet 104: 709-719.
2. Buchowicz J, Krysiak C (2003) Genotoxic effects of tungsten microprojectiles under conditions of biolistic transformation. In: Jacson JF, Linskens HF (eds) Genetic transformation of plants, Vol 23. Springer, New York, pp 175-188.
3. Carmody WR (1961) An easily prepared wide range buffer series. J Chem Educ 38: 559-561.
4. Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr Biol 6: 325-330.
5. Grenoble DC, Kim CJ, Murrell LL (1983) Hydrocarbon conversion processes using improved transition metal oxide catalysts. Exxon Research and Engineering Co. Florham Park.
6. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387-405.
7. Kosugi S, Ohashi Y, Nakajima K, Arai Y (1990) An improved assay for β-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Sci 70: 133-140.
8. Krysiak C, Mazus B, Buchowicz J (1999a) Generation of DNA double-strand breaks and inhibition of somatic embryogenesis by tungsten microparticles in wheat. Plant Cell Tiss Organ Cult 58: 163-170.
9. Krysiak C, Mazus B, Buchowicz J (1999b) Relaxation, linearization and fragmentation of supercoiled circular DNA by tungsten microprojectiles. Transgenic Res 8: 303-306.
10. Mazuś B, Krysiak C, Buchowicz J (2000) Tungsten particle-induced nicking of supercoiled plasmid DNA. Plasmid 44: 89-93.
11. Nandadeva YL, Lupi CG, Meyer CS, Devi PS, Potrykus I, Bilang R (1999) Microprojectile-mediated transient and integrative transformation of rice embryogenic suspension cells: effects of osmotic cell conditioning and of the physical configuration of plasmid DNA. Plant Cell Rep 18: 500-504.
12. Rasco-Gaunt S, Barcelo P (1999) Immature inflorescence culture of cereals. A highly responsive system for regeneration and transformation. Methods Mol Biol 111: 71-81.
13. Russell JA, Roy MK, Sanford JC (1992) Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol 98: 1050-1056.
14. Sanford JC, Smith FD, Russell JA (1993) Optimizing the biolistic process for different biological applications. Methods Enzymol 217: 483-509.
15. Shapiro HS (1967) The preparation of pyrimidine oligonucleotides by acid hydrolysis of DNA. Methods Enzymol 12A: 205-212.
16. Southgate EM, Davey MR, Power JB, Marchant R (1995) Factors affecting the genetic engineering of plants by microprojectile bombardment. Biotechnol Adv 13: 631-651.
17. Tanaka K, Asami T, Yoshida S, Nakamura Y, Matsuo T, Okamoto S (2005) Brassinosteroid homeostasis in Arabidopsis is ensured by feedback expressions of multiple genes involved in its metabolism. Plant Physiol 138: 1117-1125.
18. Thomas R, Moulijn JA, De Beer VHJ, Medema J (1980) Structure/metathesis-activity relations of silica supported molybdenum and tungsten oxide. J Mol Cat 8: 161-174.
19. Veluthambi K, Gupta AK, Sharma A (2003) The current status of plant transformation technologies. Curr Sci 84: 368-380.