Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis

PLoS ONE

Volumn 4, Issue 11, 2009, Pages

  Smith, Steven G ^a   Joosten, Simone A ^b   Verscheure, Virginie ^c   Pathan, Ansar A ^d   McShane, Helen ^d   Ottenhoff, Tom H M ^b   Dockrell, Hazel M ^a   Mascart, Françoise ^c  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c ERASME HOSPITAL   (Belgium)

^d NIHR Biomedical Researcg Centre   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; MYCOBACTERIUM ANTIGEN; BACTERIAL ANTIGEN; BCG VACCINE;

ARTICLE; CELL CULTURE; CELL STIMULATION; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; ENZYME LINKED IMMUNOSPOT ASSAY; HUMAN; HUMAN CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; QUANTITATIVE ANALYSIS; AUTOMATION; CHEMISTRY; CLINICAL CHEMISTRY; COMPUTER PROGRAM; CYTOLOGY; DENSITY GRADIENT CENTRIFUGATION; DIAGNOSIS, MEASUREMENT AND ANALYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; INSTRUMENTATION; METABOLISM; METHODOLOGY; MONONUCLEAR CELL; MYCOBACTERIUM TUBERCULOSIS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TIME;

MYCOBACTERIUM TUBERCULOSIS;

ANTIGENS, BACTERIAL; AUTOMATION; CENTRIFUGATION, DENSITY GRADIENT; CHEMISTRY, CLINICAL; CRYOPRESERVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; LABORATORY TECHNIQUES AND PROCEDURES; LEUKOCYTES, MONONUCLEAR; MYCOBACTERIUM TUBERCULOSIS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOFTWARE; TIME FACTORS; TUBERCULOSIS VACCINES;

EID: 71049189284     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0007972     Document Type: Article

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