Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Scanlon, Thomas C ^a   Gray, Elizabeth C ^b   Griswold, Karl E ^a,b  

^a DARTMOUTH COLLEGE   (United States)

^b DARTMOUTH COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AVERAGE NUMBERS; BACTERIAL SYSTEMS; CELL SURFACE DISPLAYS; DNA SEQUENCING; ESCHERICHIA COLI CELLS; GENE LIBRARIES; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; LIBRARY SCREENING; MODEL STUDY; MOLECULAR MACHINERY; MUTANT GENES; PROTEIN ENGINEERING; QUANTITATIVE INFORMATION; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; TRANSFORMANTS; TRANSFORMATION EFFICIENCY; YEAST CELL;

BACTERIOLOGY; BIOCHEMICAL ENGINEERING; BIOINFORMATICS; CELL MEMBRANES; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; MACHINERY; NUCLEIC ACIDS; POPULATION STATISTICS; PROTEINS; TRANSCRIPTION; YEAST;

VECTORS;

PLASMID DNA; FUNGAL DNA;

ARTICLE; BACTERIAL GROWTH; BACTERIUM TRANSFORMATION; CONTROLLED STUDY; DNA SEQUENCE; DNA VECTOR; GENE LIBRARY; HOMOLOGOUS RECOMBINATION; MOLECULAR CLONING; MUTANT; NONHUMAN; QUANTITATIVE ANALYSIS; SACCHAROMYCES CEREVISIAE; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; PLASMID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; SACCHAROMYCES CEREVISIAE;

DNA, FUNGAL; GENE LIBRARY; GENETIC VECTORS; PLASMIDS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

EID: 71049171721     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-95     Document Type: Article

References (21)

1. Dower WJ, Miller JF, Ragsdale CW. High-efficiency Transformation of Escherchia coli by High-voltage Electroporation. Nucl Acids Res 1988, 16(13):6127-6145. 10.1093/nar/16.13.6127, 336852, 3041370.
2. Goldsmith M, Kiss C, Bradbury ARM, Tawfik DS. Avoiding and controlling double transformation artifacts. Protein Engineering, Design and Selection 2007, 20(7):315-318.
3. Austin S, Nordström K. Partition-mediated incompatibility of bacterial plasmids. Cell 1990, 60(3):351-354. 10.1016/0092-8674(90)90584-2, 2406018.
4. Ghosh SK, Hajra S, Paek A, Jayaram M. Mechanisms for Chromosome and Plasmid Segregation. Annual Review of Biochemistry 2006, 75(1):211-241. 10.1146/annurev.biochem.75.101304.124037, 16756491.
5. Altamirano MM, Blackburn JM, Aguayo C, Fersht AR. Retraction: Directed evolution of new catalytic activity using the α/β-barrel scaffold. Nature 2002, 417(6887):468-468. 10.1038/417468a, 12024219.
6. Zeytun A, Jeromin A, Scalettar BA, Waldo GS, Bradbury ARM. Retraction: Fluorobodies combine GFP fluorescence with the binding characteristics of antibodies. Nat Biotech 2004, 22(5):601-601.
7. Dwyer MA, Looger LL, Hellinga HW. Retraction. Science 2008, 319(5863):569. 10.1126/science.319.5863.569b, 18239106.
8. Velappan N, Sblattero D, Chasteen L, Pavlik P, Bradbury ARM. Plasmid incompatibility: more compatible than previously thought?. Protein Engineering, Design and Selection 2007, 20(7):309-313.
9. Hicks JB, Hinnen A, Fink GR. Properties of Yeast Transformation. Cold Spring Harbor Symp Quant Biol 1978, 43:1305-1313.
10. Futcher B, Carbon J. Toxic effects of excess cloned centromeres. Mol Cell Biol 1986, 6(6):2213-2222. 367762, 3537715.
11. Herman A, Tawfik DS. Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries. Protein Engineering, Design and Selection 2007, 20(5):219-226.
12. Chao G, Lau WL, Hackel BJ, Sazinsky SL, Lippow SM, Wittrup KD. Isolating and engineering human antibodies using yeast surface display. Nat Protocols 2006, 1(2):755-768.
13. Burnham KP, Overton WS. Robust Estimation of Population Size When Capture Probabilities Vary Among Animals. Ecology 1979, 60(5):927-936.
14. Clarke L, Carbon J. The Structure and Function of Yeast Centromeres. Annual Review of Genetics 1985, 19(1):29-55. 10.1146/annurev.ge.19.120185.000333, 3909945.
15. Swers JS, Kellogg BA, Wittrup KD. Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display. Nucl Acids Res 2004, 32(3):15.
16. Frazer LN, O'Keefe RT. A new series of yeast shuttle vectors for the recovery and identification of multiple plasmids from Saccharomyces cerevisiae. Yeast 2007, 24(9):777-789. 10.1002/yea.1509, 17597491.
17. Polizzi KM, Parikh M, Spencer CU, Matsumura I, Lee JH, Realff MJ, Bommarius AS. Pooling for Improved Screening of Combinatorial Libraries for Directed Evolution. Biotechnology Progress 2006, 22(4):961-967. 10.1021/bp060099z, 16889370.
18. Mumberg D, Muller R, Funk M. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucl Acids Res 1994, 22(25):5767-5768. 10.1093/nar/22.25.5767, 310147, 7838736.
19. Oldenburg KR, Vo KT, Michaelis S, Paddon C. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucl Acids Res 1997, 25(2):451-452. 10.1093/nar/25.2.451, 146432, 9016579.
20. Agresti A, Coull BA. Approximate is better than "exact" for interval estimation of binomial proportions. Am Stat 1998, 52(2):119-126.
21. Samuels ML, Witmer JA. Statistics for the Life Sciences. 2003, Upper Saddle River: Pearson Education, Inc, 3.