Automated organelle-based colocalization in whole-cell imaging

Cytometry Part A

Volumn 75, Issue 11, 2009, Pages 941-950

  Woodcroft, Ben J ^a   Hammond, Luke ^a   Stow, Jennifer L ^a   Hamilton, Nicholas A ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Cell biology; Colocalization; Confocal microscopy; Fluorescent imaging; Image analysis; Image segmentation; Organelle

Indexed keywords

CELLUBREVIN; DEXTRAN; EPIDERMAL GROWTH FACTOR; RAB11 PROTEIN; TRANSFERRIN;

ANIMAL CELL; ARTICLE; CELL ASSAY; CHEMICAL LABELING; CONTROLLED STUDY; ENDOSOME; FLUORESCENCE ANALYSIS; IMAGE ANALYSIS; IMAGING SYSTEM; MAMMAL CELL; MICROSCOPY; MOUSE; NONHUMAN; ORGANELLE BASED COLOCALIZATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; STATISTICAL ANALYSIS; THREE DIMENSIONAL IMAGING;

3T3 CELLS; ANIMALS; AUTOMATION; CERCOPITHECUS AETHIOPS; COS CELLS; DEXTRANS; ENDOSOMES; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYES; IMAGING, THREE-DIMENSIONAL; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; ORGANELLES;

EID: 71049145803     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20786     Document Type: Article

References (21)

1. Li Q, Lau A, Morris TJ, Guo L, Fordyce CB, Stanley EF. A Syntaxin 1, G{alpha}o, and N-type calcium channel complex at a presynaptic nerve terminal: Analysis by quantitative immunocolocalization. J Neurosci 2004;24:4070-4081. (Pubitemid 38544233)
2. Demandolx D, Davoust J. Multicolour analysis and local image correlation in confocal microscopy. J Microsc 1997;185:21-36. (Pubitemid 27060523)
3. Manders EMM, Verbeek FJ, Aten JA. Measurement of co-localisation of objects in dual-colour confocal images. J Microsc 1993;169:375-382.
4. Costes SV, Daelemans D, Cho EH, Dobbin Z, Pavlakis G, Lockett S. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J 2004;86:3993-4003. (Pubitemid 38780275)
5. Bolte S, Cordelieres FP. A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 2006;224:213-232. (Pubitemid 46010930)
6. Manderson AP, Kay JG, Hammond LA, Brown DL, Stow JL. Subcompartments of the macrophage recycling endosome direct the differential secretion of IL-6 and TNFalpha. J Cell Biol 2007;178:57-69.
7. Van Ijzendoorn SC. Recycling endosomes. J Cell Sci 2006;119:1679-1681.
8. Thompson A, Nessler R, Wisco D, Anderson E, Winckler B, Sheff D. Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains. Mol Biol Cell 2007;18:2687-2697. (Pubitemid 47025733)
9. Cancino J, Torrealba C, Soza A, Yuseff MI, Gravotta D, Henklein P, Rodriguez-Boulan E, Gonzalez A. Antibody to AP1B adaptor blocks biosynthetic and recycling routes of basolateral proteins at recycling endosomes. Mol Biol Cell 2007;18:4872-4884. (Pubitemid 350246689)
10. Gniadek TJ, Warren G. WatershedCounting3D: A new method for segmenting and counting punctate structures from confocal image data. Traffic 2007;8:339-346. (Pubitemid 46421616)
11. Volocity 3D imaging software. Improvision, Coventry, England.
12. Pinidiyaarachchi A, Zieba A, Allalou A, Pardali K, Wahlby C. A detailed analysis of 3D subcellular signal localization. Cytometry Part A 2009;75A:319-328.
13. Rasband WS. ImageJ. Bethesda, Maryland: US National Institutes of Health. 1997- 2009; http://rsb.info.nih.gov/ij/
14. Misaki R, Nakagawa T, Fukuda M, Taniguchi N, Taguchi T. Spatial segregation of degradation- and recycling-trafficking pathways in COS-1 cells. Biochem Biophys Res Commun 2007;360:580-585. (Pubitemid 47048139)
15. Landmann L. Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques. J Microsc 2002;208:134-147. (Pubitemid 35356985)
16. Vincent L, Soille P. Watersheds in digital spaces: An efficient algorithm based on immersion simulations. IEEE Trans Pattern Anal Mach Intell 1991;13:583-598. (Pubitemid 21675616)
17. Wang G, Xiao L, He A. A sub-pixel multifractal method for the image segmentation. Fourth International Conference on Fuzzy Systems and Knowledge Discovery (FSKD 2007). IEEE Computer Society 2007;4:566-572.
18. Ridler TW, Calvard S. Picture thresholding using an iterative selection method. IEEE Trans Syst Man Cybern 1978;8:630-632. (Pubitemid 8623043)
19. Java Technology. Santa Clara, CA: Sun Microsystems Inc.
20. Henry L, Sheff DR. Rab8 regulates basolateral secretory, but not recycling, traffic at the recycling endosome. Mol Biol Cell 2008;19:2059-2068.
21. Woodman PG, Futter CE. Multivesicular bodies: Co-ordinated progression to maturity. Curr Opin Cell Biol 2008;20:408-414.