Extraction of RNA from small amounts of amphioxus embryos for cDNA synthesis by RT-PCR

Cold Spring Harbor Protocols

Volumn 4, Issue 9, 2009, Pages

  Yu, J K Sky ^a   Holland, Linda Z ^b  

^a INSTITUTE OF CELLULAR AND ORGANISMIC BIOLOGY   (Taiwan)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; RNA;

AMPHIOXUS; ARTICLE; CHORDATA; EMBRYO CULTURE; METHODOLOGY; MOLECULAR BIOLOGY; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA ISOLATION;

BRANCHIOSTOMA LANCEOLATUM;

EID: 70949106872     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5289     Document Type: Article

References (3)

1. Ransick A. 2004. Detection of mRNA by in situ hybridization and RT-PCR. Methods Cell Biol 74: 601-620.
2. Yu JKS, Holland LZ. 2009a. Cephalochordates (amphioxus or lancelets): A model for understanding the evolution of chordate characters. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.emo130.
3. Yu JKS, Holland LZ. 2009b. Extraction of RNA from amphioxus embryos or adult amphioxus tissue. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5288.