Whole-mount in situ hybridization of hawaiian bobtail squid (euprymna scolopes) embryos with DIG-labeled riboprobes: I. DNA template preparation and in vitro transcription of riboprobes

Cold Spring Harbor Protocols

Volumn 4, Issue 11, 2009, Pages

  Lee, Patricia N ^a,b   McFall Ngai, Margaret J ^c   Callaerts, Patrick ^d   de Couet, H Gert ^a  

^a UNIVERSITY OF HAWAII   (United States)

^b UNIVERSITY OF HAWAII   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

^d UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; DIGOXIGENIN; MESSENGER RNA;

ARTICLE; COLORIMETRY; CONCENTRATION (PARAMETERS); EMBRYONAL TISSUE; EUPRYMNA SCOLOPES; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; RNA PROBE; RNA SEQUENCE; SQUID; TEMPERATURE; TISSUE FIXATION; UNITED STATES;

EUPRYMNA SCOLOPES; SEPIOLIDAE;

EID: 70949101897     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5321     Document Type: Article

References (2)

1. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. 2009a. Whole-mount in situ hybridization of Hawaiian bobtail squid (Euprymna scolopes) embryos with DIG-labeled riboprobes: II. Embryo preparation, hybridization, washes, and immunohistochemistry. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5322.
2. Lee PN, McFall-Ngai MJ, Callaerts P, de Couet HG. 2009b. The Hawaiian bobtail squid (Euprymna scolopes): A model to study the molecular basis of eukaryote-prokaryote mutualism and the development and evolution of morphological novelties in cephalopods. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.emo135.