Bimolecular affinity purification (BAP): Tandem affinity purification using two protein baits

Cold Spring Harbor Protocols

Volumn 4, Issue 11, 2009, Pages

  Maine, Gabriel N ^a   Gluck, Nathan ^b   Zaidi, Iram W ^c   Burstein, Ezra ^c,d  

^a WILLIAM BEAUMONT HOSPITAL   (United States)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^d UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYLTRANSFERASE; GLUTATHIONE TRANSFERASE; PLASMID DNA; TRANSCRIPTION FACTOR RELA;

ARTICLE; BIOMOLECULAR AFFINITY PURIFICATION; GENETIC TRANSFECTION; MOLECULAR CLONING; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; QUALITY CONTROL; TANDEM AFFINITY PURIFICATION; WESTERN BLOTTING;

MAMMALIA;

EID: 70949087273     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5318     Document Type: Article

References (5)

1. Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, Superti-Furga G, Bauch A. 2006. An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells. Nat Methods 3: 1013-1019.
2. Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, Burstein E. 2009. GCN5 is a required cofactor for a ubiquitin ligase that targets NF-κ/RelA. Genes & Dev 23: 849-861.
3. Puig O, Caspary F, Rigaut G, Rutz B, Bouveret E, Bragado-Nilsson E, Wilm M, Seraphin B. 2001. The tandem affinity purification (TAP) method: A general procedure of protein complex purification. Methods 24: 218-229.
4. Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17: 1030-1032.
5. Sambrook J, Russell DW. 2006. Calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNAs. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3871.