Molecular cloning and expression of the Trichoderma harzianum C4 endo-β-1,4-xylanase gene in Saccharomyces cerevisiae

Journal of Microbiology and Biotechnology

Volumn 19, Issue 8, 2009, Pages 823-828

  Lee, Jung Min ^a   Shin, Ji Won ^a   Nam, Jae Kook ^a   Choi, Ji Young ^b   Jeong, Choon Soo ^b   Han, In Seob ^b   Nam, Soo Wan ^c   Choi, Yun Jaie ^d   Chung, Dae Kyun ^a  

^a Kyung Hee University   (South Korea)

^b University of Ulsan   (South Korea)

^c Dong Eui University   (South Korea)

^d Seoul National University   (South Korea)

Author keywords

Endo 1,4 xylanase; Saccharomyces cerevisiae; Trichoderma harzianum

Indexed keywords

ALCOHOL DEHYDROGENASE; ALCOHOL DEHYDROGENASE 1; COMPLEMENTARY DNA; ENDO 1,4 BETA XYLANASE; GENOMIC DNA; PHOSPHOGLYCERATE KINASE; PHOSPHOGLYCERATE KINASE 1; UNCLASSIFIED DRUG;

ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC CODE; HYPOCREA LIXII; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, FUNGAL; ENDO-1,4-BETA XYLANASES; INDUSTRIAL MICROBIOLOGY; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TRICHODERMA;

HYPOCREA LIXII; SACCHAROMYCES CEREVISIAE;

EID: 70849087555     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0810.577     Document Type: Article

References (20)

1. Biely, P., C. R. Mackenzie, J. Plus, and H. Schneider. 1986. Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Bio/Technology 4: 731-733. (Pubitemid 17218634)
2. Calmels, T. P. G., F. Martin, H. Durand, and G. Tiraby. 1991. Proteolytic events in processing of secreted proteins in fungi. J. Biotechnol. 17: 51-66.
3. Collins, T., C. Gerday, and G. Feller. 2005. Xylanases, xylanases families and extremophilic xylanases. FEMS Microbiol. Rev. 29: 3-23.
4. Denis, C. L., J. Ferguson, and E. T. Young. 1983. mRNA levels for the fermentative alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon source. J. Biol. Chem. 258: 1165-1171.
5. Goller, S. P., D. Schoisswohl, M. Baron, M. Parric, and C. P. Kubicek. 1998. Role of endoproteolytic dibasic protein processing in maturation of secretory proteins in Trichoderma reesei. Appl. Environ. Microbiol. 64: 3202-3208.
6. de Vries, R. P., H. C. Kester, C. H. Polusen, J. A. Benen, and J. Visser. 2000. Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides. Carbohydr. Res. 327: 402-410.
7. Hardjito, L., P. F. Greenfield, and P. L. Lee. 1993. Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae. Enzyme Microbiol. Technol. 15: 120-126.
8. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1952. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
9. Mandels, M. and R. E. Anderotti. 1978. The cellulose to cellulase fermentation. Proc. Biochem. 13: 6-113.
10. Nacken, V., T. Achstetter, and E. Degryse. 1996. Probing the limits of expression levels by varying promoter strength and plasmid copy number in Saccharomyces cerevisiae. Gene 175: 253-260. (Pubitemid 26363027)
11. Polizeli, M. L. T. M., A. C. S. Rizzatti, R. Monti, H. F. Terenzi, J. A. Jorge, and D. S. Amorim. 2005. Xylanases from fungi: Properties and industrial applications. Appl. Microbiol. Biotechnol. 67: 577-591.
12. Pronk, J. T. 2002. Auxotrophic strains in fundamental and applied research. Appl. Environ. Microbiol. 68: 2095-2100.
13. Uffen, R. L. 1997. Xylan degradation: A glimpse at microbial diversity. J. Ind. Microbiol. Biotech. 19: 1-6.
14. Sengupta, S., M. L. Jana, D. Sengupta, and A. K. Naskar. 2000. A note on the estimation of microbial glycosidase activities by dinitrosalicylic acid reagent. Appl. Microbiol. Biotechnol. 53: 732-735.
15. Shirahama, T. and A. S. Cohen. 1966. A Congo red staining method for epoxy-embeded amyloid. J. Histochem. Cytochem. 14: 725-729.
16. Son, Y. J., O. J. Sul, D. K. Chung, I. S. Han, Y. J. Choi, and C. S. Jeong. 1997. Isolation and characterization of Trichoderma sp. C-4 producing cellulase. Kor. J. Appl. Microbiol. Biotechnol. 25: 346-353.
17. Thomson, J. A. 1993. Molecular biology of xylan degradation. FEMS Microbiol. Rev. 104: 65-82.
18. Torronen, A., R. L. Mach, R. Messner, R. Gonzalez, N. Kalkkinen, A. Harkki, and C. P. Kubicek. 1992. The two major xylanases from Trichoderma reesei: Characterization of both enzymes and genes. Biotechnology 10: 1461-1466.
19. Torrone, A and J. Rouvine. 1995. Structural comparison of two major endo-1,4-xylanases from Trichoderma reesei. Biochemistry 34: 847-856.
20. Yaguchi, M., C. Roy, D. C. Watson, F. Rollin, L. U. L. Tan, D. J. Senior, and J. N. Saddler. 1992. The amino acid sequence of the 20 kd xylanase from Trichoderma harzianum E58, pp. 435-438. In J. Visser (ed.), Xylans and Xylanases, Elsevier, Amsterdam.