Diagnostic utility of polymerase chain reaction on intraocular specimens to establish the etiology of infectious endophthalmitis

European Journal of Ophthalmology

Volumn 19, Issue 5, 2009, Pages 812-817

  Sownya, Parameswaran ^a   Madhavan, Hajib N ^a  

^a VISION RESEARCH FOUNDATION   (India)

Author keywords

Aqueous humor; Infectious endophthalmitis; Polymerase chain reaction; Vitreous fluid

Indexed keywords

ACINETOBACTER CALCOACETICUS; ALCALIGENES FAECALIS; AQUEOUS HUMOR; ARTICLE; ASPERGILLUS NIGER; ASPERGILLUS TERREUS; BACTERIAL GENOME; BACTERIUM CULTURE; BACTERIUM ISOLATE; CONTROLLED STUDY; CORYNEBACTERIUM; DIAGNOSTIC VALUE; ENDOPHTHALMITIS; ENTEROCOCCUS FAECALIS; FALSE POSITIVE RESULT; FUNGAL GENOME; FUNGUS CULTURE; FUSARIUM; HUMAN; MAJOR CLINICAL STUDY; MYCOBACTERIUM ABSCESSUS; MYCOLOGY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS; VITREOUS BODY;

AQUEOUS HUMOR; BACTERIA; CATARACT EXTRACTION; DNA, BACTERIAL; DNA, FUNGAL; ENDOPHTHALMITIS; EYE INFECTIONS, BACTERIAL; EYE INFECTIONS, FUNGAL; FUNGI; HUMANS; POLYMERASE CHAIN REACTION; POSTOPERATIVE COMPLICATIONS; VITREOUS BODY;

EID: 70549085964     PISSN: 11206721     EISSN: None     Source Type: Journal    
DOI: 10.1177/112067210901900520     Document Type: Article

References (18)

1. Therese KL, Anand AR, Madhavan HN. Polymerase chain reaction in the diagnosis of bacterial endophthalmitis. Br J Ophthalmol 1998; 82: 1078-82.
2. Bagyalakshmi R, Madhavan HN, Therese KL. Development and application of multiplex polymerase chain reaction for the etiologicai diagnosis of infectious endophthalmitis. J Postgrad Med 2006; 52: 179-82.
3. Endophthalmitis Vitrectomy Study. Microbiologic factors and visual outcome in the Endophthalmitis Vitrectomy Study. Am J Ophthalmol 1996; 122: 830-46.
4. Okhravi N, Adamson P, Carroll N, et al. PCR-based evidence of bacterial involvement in eyes with suspected intraocular infection. Tnvest Ophthalmol Vis Sci 2000; 41: 3474-9.
5. Therese KL, Bartell JG, Bagyalakshmi R, Dajcs JG, Stroman DW, Madhavan HN. Etiology of endophthalmitis from culture-negative specimens. Invest Ophthalmol Vis Sci 2006; 47: e-abstract 1890.
6. Rothova A, Ooijman F, Kerkhoff F, Van Der Lelij A, Lokhorst Hm. Uveitis masquerade syndromes. Ophthalmology 2001; 108: 386-99.
7. Kresloff MS, Castellarin AA, Zarbin MA. Endophthalmitis. Surv Ophthalmol 1998; 43: 193-224.
8. Busin M, Cusumano A, Spitznas M. Intraocular lens removal from eyes with chronic low-grade endophthalmitis. J Cataract Refract Surg 1995; 21: 679-84.
9. Bagyalakshmi R, Therese KL, Madhavan HN. Application of semi-nested polymerase chain reaction targeting internal transcribed spacer region for rapid detection of panfungal genome directly from ocular specimens. Indian J Ophthalmol 2007; 55: 261-5.
10. Carroll NM, Jaeger EE, Choudhury S, et al. Detection of and discrimination between gram-positive and gram negative bacteria in intraocular samples by using nested PCR. J Clin Microbiol 2000; 38: 1753-7.
11. Chiquet C, Cornut PL, Benito Y, et al. Eubacterial PCR for bacterial detection and identification in 100 acute postcataract surgery endophthalmitis: Invest Ophthalmol Vis Sci 2008; 49: 1971-8.
12. Seal D, Reischl U, Behr A, et al. Laboratory diagnosis of endophthalmitis: comparison of microbiology and molecular methods in the European Society of Cataract & Refractive Surgeons multicenter study and susceptibility testing. J Cataract Refract Surg 2008; 34: 1439-50.
13. Druel B, Vandenesch F, Greenland T, et al. Aseptic meningitis after neurosurgery: a demonstration of bacterial involvement. Clin Microbiol Ipfect 1996; 1: 230-4.
14. Anand AR, Therese KL, Madhavan HN. Spectrum of aetological agents of postoperative endophthalmitis and antibiotic susceptibility of bacterial isolates. Indian J Ophthalmol 2000; 48: 123-8.
15. Kunimoto DY, Das T, Sharma S, et al. Microbiologic spectrum and susceptibility of isolates: part II. Posttraumatic endophthalmitis. Endophthalmitis Research Group. Am J Ophthalmol 1999; 128: 242-4.
16. Bourcier T, Scheer S, Chaumeil C, Morel C, Borderie V, Laroche L. Fungal and bacterial chronic endophthalmitis following cataract surgery Br J Ophthalmol 2003; 87: 372-3.
17. Kunimoto DY, Das T, Sharma S, et al. Microbiologic spectrum and susceptibility of isolates: part I. Postoperative endophthalmitis. Endophthalmitis Research Group. Am J Ophthalmol 1999; 128: 240-2.
18. Okhravi N, Adamson P, Lightman S. Use of PCR in endophthalmitis. Ocul Immunol Inflamm 2000; 8: 189-200.