Dynasore, a dynamin inhibitor, suppresses lamellipodia formation and cancer cell invasion by destabilizing actin filaments

Biochemical and Biophysical Research Communications

Volumn 390, Issue 4, 2009, Pages 1142-1148

  Yamada, Hiroshi ^a   Abe, Tadashi ^a   Li, Shun Ai ^a   Masuoka, Yuki ^a   Isoda, Mihoko ^a   Watanabe, Masami ^a   Nasu, Yasutomo ^a   Kumon, Hiromi ^a   Asai, Akira ^b   Takei, Kohji ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b UNIVERSITY OF SHIZUOKA   (Japan)

Author keywords

Actin cytoskeleton; Cortactin; Dynamin; Dynasore; Invasion; Lamellipodia

Indexed keywords

ANTINEOPLASTIC AGENT; CORTACTIN; DYNAMIN; DYNAMIN II; DYNASORE; ENZYME INHIBITOR; F ACTIN; UNCLASSIFIED DRUG;

ACTIN FILAMENT; ARTICLE; CANCER CELL; CANCER INHIBITION; CANCER INVASION; CELL INVASION; CELL MEMBRANE; CONTROLLED STUDY; CYTOSOL; HUMAN; HUMAN CELL; IN VITRO STUDY; LAMELLIPODIUM; LUNG CANCER; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN STABILITY;

ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; CORTACTIN; DYNAMIN II; HUMANS; HYDRAZONES; LUNG NEOPLASMS; MICROFILAMENTS; NEOPLASM INVASIVENESS; PSEUDOPODIA;

EID: 70450285164     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.10.105     Document Type: Article

References (25)

1. Pollard T.D., and Borisy G.G. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112 (2003) 453-465
2. Ammer A.G., and Weed S.A. Cortactin branches out: roles in regulating protrusive actin dynamics. Cell Motil. Cytoskeleton 65 (2008) 687-707
3. Praefcke G.J., and McMahon H.T. The dynamin superfamily: universal membrane tubulation and fission molecules?. Nat. Rev. Mol. Cell Biol. 5 (2004) 133-147
4. Takei K., Slepnev V.I., Haucke V., and De Camilli P. Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis. Nat. Cell Biol. 1 (1999) 33-39
5. Torre E., McNiven M.A., and Urrutia R. Dynamin 1 antisense oligonucleotide treatment prevents neurite formation in cultured hippocampal neurons. J. Biol. Chem. 269 (1994) 32411-32417
6. Ochoa G.C., Slepnev V.I., Neff L., Ringstad L., Takei K., Daniell L., Kim W., Cao H., McNiven M.A., Baron R., and De Camilli P. A functional link between dynamin and the actin cytoskeleton at podosomes. J. Cell Biol. 150 (2000) 377-389
7. Baldassarre M., Pompeo A., Beznoussenko G., Castaldi C., Cortellino S., McNiven M.A., Luini A., and Buccione R. Dynamin participates in focal extracellular matrix degradation by invasive cells. Mol. Biol. Cell 14 (2003) 1074-1084
8. Cao H., Garcia F., and McNiven M.A. Differential distribution of dynamin isoforms in mammalian cells. Mol. Biol. Cell 9 (1998) 2595-2609
9. Cao H., Orth J.D., Chen J., Weller S.G., Heuser J.E., and McNiven M.A. Cortactin is a component of clathrin-coated pits and participates in receptor-mediated endocytosis. Mol. Cell. Biol. 23 (2003) 2162-2170
10. McNiven M.A., Kim L., Krueger E.W., Orth J.D., Cao H., and Wong T.W. Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape. J. Cell Biol. 151 (2000) 187-198
11. Gold E.S., Underhill D.M., Morrissette N.S., Guo J., McNiven M.A., and Aderem A. Dynamin 2 is required for phagocytosis in macrophages. J. Exp. Med. 190 (1999) 1849-1856
12. Otsuka A., Abe T., Watanabe M., Yagisawa H., Takei K., and Yamada H. Dynamin 2 is required for actin assembly in phagocytosis in Sertoli cells. Biochem. Biophys. Res. Commun. 378 (2009) 478-482
13. Damke H., Baba T., Warnock D.E., and Schmid S.L. Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. J. Cell Biol. 127 (1994) 915-934
14. Schafer D.A., Weed S.A., Binns D., Karginov A.V., Parsons J.T., and Cooper J.A. Dynamin2 and cortactin regulate actin assembly and filament organization. Curr. Biol. 12 (2002) 1852-1857
15. Mooren O.L., Kotova T.I., Moore A.J., and Schafer D.A. Dynamin2 GTPase and cortactin remodel actin filaments. J. Biol. Chem. 284 (2009) 23995-24005
16. Macia E., Ehrlich M., Massol R., Boucrot E., Brunner C., and Kirchhausen T. Dynasore, a cell-permeable inhibitor of dynamin. Dev. Cell 10 (2006) 839-850
17. Kirchhausen T., Macia E., and Pelish H.E. Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis. Methods Enzymol. 438 (2008) 77-93
18. Kruchten A.E., Krueger E.W., Wang Y., and McNiven M.A. Distinct phospho-forms of cortactin differentially regulate actin polymerization and focal adhesions. Am. J. Physiol. Cell Physiol. 295 (2008) C1113-C1122
19. H. Yamada, S. Padilla-Parra, S.J. Park, T. Itoh, M. Chaineau, I. Monaldi, O. Cremona, F. Benfenati, P. De Camilli, M. Coppey-Moisan, M. Tramier, T. Galli, K. Takei, Dynamic interaction of amphiphysin with N-wasp regulates actin assembly, J. Biol. Chem., in press.
20. Edamura K., Nasu Y., Takaishi M., Kobayashi T., Abarzua F., Sakaguchi M., Kashiwakura Y., Ebara S., Saika T., Watanabe M., Huh N.H., and Kumon H. Adenovirus-mediated REIC/Dkk-3 gene transfer inhibits tumor growth and metastasis in an orthotopic prostate cancer model. Cancer Gene Ther. 14 (2007) 765-772
21. Yamada H., Ohashi E., Abe T., Kusumi N., Li S.A., Yoshida Y., Watanabe M., Tomizawa K., Kashiwakura Y., Kumon H., Matsui H., and Takei K. Amphiphysin 1 is important for actin polymerization during phagocytosis. Mol. Biol. Cell 18 (2007) 4669-4680
22. Saito S., Watabe S., Ozaki H., Fusetani N., and Karaki H. Mycalolide B, a novel actin depolymerizing agent. J. Biol. Chem. 269 (1994) 29710-29714
23. Hara T., Honda K., Shitashige M., Ono M., Matsuyama H., Naito K., Hirohashi S., and Yamada T. Mass spectrometry analysis of the native protein complex containing actinin-4 in prostate cancer cells. Mol. Cell. Proteomics 6 (2007) 479-491
24. Gareus R., Di Nardo A., Rybin V., and Witke W. Mouse profilin 2 regulates endocytosis and competes with SH3 ligand binding to dynamin 1. J. Biol. Chem. 281 (2006) 2803-2811
25. Kessels M.M., Engqvist-Goldstein A.E., Drubin D.G., and Qualmann B. Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin. J. Cell Biol. 153 (2001) 351-366