Probing mechanisms for enzymatic activity enhancement of organophosphorus hydrolase in functionalized mesoporous silica

Biochemical and Biophysical Research Communications

Volumn 390, Issue 4, 2009, Pages 1177-1181

  Chen, Baowei ^a   Lei, Chenghong ^a   Shin, Yongsoon ^a   Liu, Jun ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

Author keywords

Conformational change; Enzyme activity; Mesoporous silica; Organophosphorus hydrolase; Spectroscopic methods

Indexed keywords

ACRYLAMIDE; ARYLDIALKYLPHOSPHATASE; FUNCTIONALIZED MESOPOROUS SILICA; SILICON DIOXIDE; SOLVENT; TRYPTOPHAN; UNCLASSIFIED DRUG; UREA;

ALPHA HELIX; ANISOTROPY; ARTICLE; CIRCULAR DICHROISM; DENSITY; ELECTRICITY; ENZYME ACTIVITY; EXPOSURE; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; STEADY STATE; X RAY ANALYSIS;

ARYLDIALKYLPHOSPHATASE; CATALYSIS; CIRCULAR DICHROISM; ENZYMES, IMMOBILIZED; POROSITY; PROTEIN STRUCTURE, SECONDARY; SILICON DIOXIDE; SPECTROMETRY, FLUORESCENCE;

EID: 70450263338     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.10.112     Document Type: Article

References (31)

1. Zhou H.X. Protein folding and binding in confined spaces and in crowded solutions. J. Mol. Recognit. 17 (2004) 368-375
2. Zhou H.-X., and Dill K.A. Stabilization of proteins in confined spaces. Biochemistry 40 (2001) 11289-11293
3. Minton A.P. The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media. J. Biol. Chem. 276 (2001) 10577-10580
4. Bhatia R.B., and Brinker C.J. Aqueous sol-gel process for protein encapsulation. Chem. Mater. 12 (2000) 2434-2441
5. Feng X., Fryxell G.E., Wang L.-Q., Kim A.Y., Liu J., and Kemner K.M. Functionalized monolayers on ordered mesoporous supports. Science 276 (1997) 923-926
6. Zhao D., Huo Q., Feng J., Chmelka B.F., and Stucky G.D. Nonionic triblock and star diblock copolymer and oligomeric surfactant syntheses of highly ordered, hydrothermally stable, mesoporous silica structures. J. Am. Chem. Soc. 120 (1998) 6024-6036
7. Zhao D.Y., Feng J.L., Huo Q.S., Melosh N., Fredrickson G.H., Chmelka B.F., and Stucky G.D. Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores. Science 279 (1998) 548-552
8. Yiu H.H.P., Wright P.A., and Botting N.P. Enzyme immobilisation using siliceous mesoporous molecular sieves. Micropor. Mesopor. Mater. 44-45 (2001) 763-768
9. Yiu H.H.P., Wright P.A., and Botting N.P. Enzyme immobilization using SBA-15 mesoporous molecular sieves with functionalised surfaces. J. Mol. Catal. B: Enzym. 15 (2001) 81-92
10. Han Y.J., Watson J.T., Stucky G.D., and Butler A. Catalytic activity of mesoporous silicate-immobilized chloroperoxidases. J. Mol. Catal. B: Enzym. 17 (2002) 1-8
11. Han Y.J., Stucky G.D., and Butler A. Mesoporous silicate sequestration and release of proteins. J. Am. Chem. Soc. 121 (1999) 9897-9898
12. Takahashi H., Li B., Sasaki T., Miyazaki C., Kajino T., and Inagaki S. Catalytic activity in organic solvents and stability of immobilized enzymes depend on the pore size and surface characteristics of mesoporous silica. Chem. Mater. 12 (2000) 3301-3305
13. Washmon L.L., and Balkus Jr. K.J. Cytochrome c immobilized into mesoporous molecular sieves. J. Mol. Catal. B: Enzym. 10 (2000) 453-469
14. Gimon-Kinsel M.E., Jimenez V.L., Washmon L.L., and Balkus Jr. K.J. Mesoporous molecular sieve immobilized enzymes. Stud. Surf. Sci. Catal. 117 (1998) 373-380
15. Diaz J.F., and Balkus Jr. K.J. Enzymes immobilized in MCM-41 molecular sieves. J. Mol. Catal. B: Enzym. 2 (1996) 115-126
16. Deere J., Magner E., Wall J.G., and Hodnett B.K. Mechanistic and structural features of protein adsorption onto mesoporous silicates. J. Phys. Chem. B 106 (2002) 7340-7347
17. Lei C., Soares T.A., Shin Y., Liu J., and Ackerman E.J. Enzyme specific activity in functionalized nanoporous supports. Nanotechnology 19 (2008) 125102
18. Lei C., Shin Y., Liu J., and Ackerman E.J. Synergetic effects of nanoporous support and urea on enzyme activity. Nano Lett. 7 (2007) 1050-1053
19. Lei C., Shin Y., Magnuson J.K., Fryxell G., Lasure L.L., Elliott D.C., Liu J., and Ackerman E.J. Characterization of functionalized nanoporous supports for protein confinement. Nanotechnology 17 (2006) 5531-5538
20. Lei C., Shin Y., Liu J., and Ackerman E.J. Entrapping enzyme in a functionalized nanoporous support. J. Am. Chem. Soc. 124 (2002) 11242-11243
21. Dunker A.K., and Fernandez A. Engineering productive enzyme confinement. Trends Biotechnol. 25 (2007) 189-190
22. Liu J., Shin Y., Nie Z.M., Chang J.H., Wang L.-Q., Fryxell G.E., Samuels W.D., and Exarhos G.J. Molecular assembly in ordered mesoporosity: a new class of highly functional nanoscale materials. J. Phys. Chem. A 104 (2000) 8328-8339
23. Chen B.W., Lowry D.F., Mayer M.U., and Squier T.C. Helix A stabilization precedes amino-terminal lobe activation upon calcium binding to calmodulin. Biochemistry 47 (2008) 9220-9226
24. Benning M.M., Shim H., Raushel F.M., and Holden H.M. High resolution X-ray structures of different metal-substituted forms of phosphotriesterase from pseudomonas diminuta. Biochemistry 40 (2001) 2712-2722
25. Benning M.M., Kuo J.M., Raushel F.M., and Holden H.M. 3-Dimensional structure of the binuclear metal center of phosphotriesterase. Biochemistry 34 (1995) 7973-7978
26. Benning M.M., Kuo J.M., Raushel F.M., and Holden H.M. 3-Dimensional structure of phosphotriesterase-an enzyme capable of detoxifying organophosphate nerve agents. Biochemistry 33 (1994) 15001-15007
27. Zheng J.Y., Constantine C.A., Rastogi V.K., Cheng T.C., DeFrank J.J., and Leblanc R.M. Secondary structure of organophosphorus hydrolase in solution and in Langmuir-Blodgett film studied by circular dichroism spectroscopy. J. Phys. Chem. B 108 (2004) 17238-17242
28. Grimsley J.K., Scholtz J.M., Pace C.N., and Wild J.R. Organophosphorus hydrolase is a remarkably stable enzyme that unfolds through a homodimeric intermediate. Biochemistry 36 (1997) 14366-14374
29. Vanhooke J.L., Benning M.M., Raushel F.M., and Holden H.M. Three-dimensional structure of the zinc-containing phosphotriesterase with the bound substrate analog diethyl 4-methylbenzylphosphonate. Biochemistry 35 (1996) 6020-6025
30. Zheng J.Y., Desbat B., Rastogi V.K., Shah S.S., DeFrank J.J., and Leblanc R.M. Organophosphorus hydrolase at the air-water interface. Secondary structure and interaction with paraoxon. Biomacromolecules 7 (2006) 2806-2810
31. Lakowicz J. Principles of Fluorescence Spectroscopy. second ed. (1999), Kluwer Academic/Plenum Publishers, New York