Automated tracking of unmarked cells migrating in three-dimensional matrices applied to anti-cancer drug screening

Experimental Cell Research

Volumn 316, Issue 2, 2010, Pages 181-193

  Adanja, Ivan ^a   Debeir, Olivier ^a   Mégalizzi, Véronique ^a   Kiss, Robert ^a   Warzée, Nadine ^a   Decaestecker, Christine ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

3D cell migration; Drug screening; Image analysis; Phase contrast microscopy; Time lapse imaging

Indexed keywords

CYTOCHALASIN D;

ARTICLE; AUTOMATION; CELL CULTURE; CELL MIGRATION; CONTROLLED STUDY; DRUG MECHANISM; DRUG SCREENING; GEL; HUMAN; HUMAN CELL; PHASE CONTRAST MICROSCOPY; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; THREE DIMENSIONAL IMAGING;

EID: 70450260556     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2009.10.004     Document Type: Article

References (25)

1. Decaestecker C., Debeir O., Van Ham P., and Kiss R. Can anti-migratory drugs be screened in vitro? A review of 2D and 3D assays for the quantitative analysis of cell migration. Med. Res. Rev. 27 (2007) 149-176
2. Debeir O., Van Ham P., Kiss R., and Decaestecker C. Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes. IEEE Trans. Med. Imaging 24 (2005) 697-711
3. Debeir O., Mégalizzi V., Warzée N., Kiss R., and Decaestecker C. Videomicroscopic extraction of specific information on cell proliferation and migration in vitro. Exp. Cell. Res. 314 (2008) 2985-2998
4. Friedl P., and Bröcker E.-B. The biology of cell locomotion within three-dimensional extracellular matrix. Cell Mol. Life Sci. 57 (2000) 41-64
5. Debeir O., Adanja I., Kiss R., and Decaestecker C. Models of cancer cell migration and cellular imaging and analysis. In: Lambrechts A., and Ampe C. (Eds). The Motile Actin System in Health and Disease (2008), Transworld research network, Kerala (India) 123-156
6. Niggemann B., Drell IV T.L., Joseph J., Weidt C., Lang K., Zaenker K.S., and Entschladen F. Tumor cell locomotion, differential dynamics of spontaneous and induced migration in a 3D collagen matrix. Exp. Cell Res. 298 (2004) 178-187
7. Grinnell F., Rocha L.B., Iucu C., Rhee S., and Jiang H. Nested collagen matrices, a new model to study migration of human fibroblast populations in three dimensions. Exp. Cell. Res. 312 (2006) 86-94
8. Li S., Lao J., Chen B.P.C., Shuan Li Y., Zhao Y., Chu J., Chen K.-D., Tsou T.-C., Peck K., and Chien S. Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix. FASEB J. 17 (2003) 97-99
9. Dufour A., Shinin V., Tajbakhsh S., Guillén-Aghion N., Olivo-Marin J.-C., and Zimmer C. Segmenting and tracking fluorescent cells in dynamic 3-D microscopy with coupled active surfaces. IEEE Trans. Image Process 14 (2005) 1396-1410
10. Soll D.R. Computer-assisted three-dimensional reconstruction and motion analysis of living, crawling cells. Comput. Med. Imaging Graph. 23 (1999) 3-14
11. Chen Y., Ladi E., Herzmark P., Robey E., and Roysam B. Automated 5-D analysis of cell migration and interaction in the thymic cortex from time-lapse sequences of 3-D multi-channel multi-photon images. J. Immunol. Methods 340 (2008) 65-80
12. Demou Z.N., and McIntire L.V. Fully automated three-dimensional tracking of cancer cells in collagen gels, determination of motility phenotypes at the cellular level. Cancer Res. 62 (2002) 5301-5307
13. Eils R., and Athale C. Computational imaging in cell biology. J. Cell Biol. 161 (2003) 477-481
14. Friedl P., and Bröcker E.-B. Reconstructing leukocyte migration in 3D extracellular matrix by time-lapse videomicroscopy and computer-assisted tracking. Methods Mol. Biol. 239 (2004) 77-90
15. Hazgui S., Bonnet N., Cutrona J., Nawrocki-Raby B., Polette M., Chouchane L., Birembaut P., and Zahm J.M. 3D culture model and computer-assisted videomicroscopy to analyze migratory behavior of noninvasive and invasive bronchial epithelial cells. Am. J. Physiol. Cell Physiol. 289 (2005) C1547-C1552
16. Hayot C., Debeir O., Van Ham P., Damme M.V., Kiss R., and Decaestecker C. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicol. Appl. Pharmacol. 211 (2006) 30-40
17. Gonzalez R.C., and Woods R.E. Digital image processing (1993), Addison-Wesley Publishing Inc
18. Cheng Y. Mean shift, mode seeking, and clustering. IEEE Trans. Pattern Anal. Mach. Intell. 17 (1995) 790-799
19. Krooshoop D.J., Torensma R., van den Bosch G.J., Nelissen J.M., Figdor C.G., Raymakers R.A., and Boezeman J.B. An automated multi well cell track system to study leukocyte migration. J. Immunol. Methods 280 (2003) 89-102
20. Martens L., Monsieur G., Ampe C., Gevaert K., and Vandekerckhove J. Cell_motility, a cross-platform, open source application for the study of cell motion paths. BMC Bioinformatics 7 (2006) 289
21. Li K., Miller E.D., Chen M., Kanade T., Weiss L.E., and Campbell P.G. Cell population tracking and lineage construction with spatiotemporal context. Med. Image Anal. 12 (2008) 546-566
22. Smal I., Meijering E., Draegestein K., Galjart N., Grigoriev I., Akhmanova A., van Royen M.E., Houtsmuller A.B., and Niessen W. Multiple object tracking in molecular bioimaging by Rao-Blackwellized marginal particle filtering. Med. Image Anal. 12 (2008) 764-777
23. Godinez W.J., Lampe M., Wörz S., Müller B., Eils R., and Rohr K. Deterministic and probabilistic approaches for tracking virus particles in time-lapse fluorescence microscopy image sequences. Med. Image Anal. 13 (2009) 325-342
24. Vallotton P., Ponti A., Waterman-Storer C.M., Salmon E.D., and Danuser G. Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. Biophys. J. 85 (2003) 1289-1306
25. Kozubek M., Matula P., Matula P., and Kozubek S. Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy. Microsc. Res. Tech. 64 (2004) 164-175